Codon Optimization and Cloning of Bovine Chymosin Gene into pTOLT Expression Plasmid of Escherichia coli

Yakup ULUSU; İsa GOKCE

Makale detayları

Codon Optimization and Cloning of Bovine Chymosin Gene into pTOLT Expression Plasmid of Escherichia coli

Yazarlar:

Yakup ULUSU | İsa GOKCE

Yayıncı:

Tokat Gaziosmanpasa University

Dergi:

Journal of New Results in Science 2012 Vol.1 Issue 1, pp.33-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000490178

Sıklık:

Üç Yıllık

pISSN:

1304-7981

Özet:

 

Chymosin is a very important industrial enzyme that commonly used in cheese manufacture. Bovine chymosin is an aspartic protease which is extracted from abomasum of suckling calves. In this study, bovine chymosin gene was first optimized and then cloned into pTOLT E.coli expression system for production of chymosin. Protein engineering of chymosin has also been attempted. A number of companies are now producing recombinant chymosin for commercial use in cheese manufacture.

Makale detayları

Codon Optimization and Cloning of Bovine Chymosin Gene into pTOLT Expression Plasmid of Escherichia coli

Yazarlar: Yakup ULUSU | İsa GOKCE

Yayıncı: Tokat Gaziosmanpasa University

Dergi: Journal of New Results in Science 2012 Vol.1 Issue 1, pp.33-39

Belge Tipi: Makale

Yayın Tarihi: 12/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000490178

Sıklık: Üç Yıllık

pISSN: 1304-7981

Sayı

Yazarlar:

Yakup ULUSU | İsa GOKCE

Yayıncı:

Tokat Gaziosmanpasa University

Dergi:

Journal of New Results in Science 2012 Vol.1 Issue 1, pp.33-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000490178

Sıklık:

Üç Yıllık

pISSN:

1304-7981