Primary Drug Resistance and Molecular Epidemiology of the Mycobacterium tuberculosis Strains Isolated in the Kelkit Valley

Yunus BULUT; Gülgün YENİŞEHİRLİ; Barış OTLU

Makale detayları

Primary Drug Resistance and Molecular Epidemiology of the Mycobacterium tuberculosis Strains Isolated in the Kelkit Valley

Yazarlar:

Yunus BULUT | Gülgün YENİŞEHİRLİ | Barış OTLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2009 Vol.39 Issue 1, pp.101-107

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000755613

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Aim: The aim of this study was to determine the primary drug resistance rates and genotypes of resistant Mycobacterium tuberculosis strains isolated in the Kelkit Valley.   Materials and Methods: Primary resistance to isoniazid (INH), rifampicin (RIF), ethambutol (ETB), and streptomycin (SM) was determined with the BACTEC 460 radiometric system. Forty-eight resistant M. tuberculosis strains isolated from tuberculosis patients living in the Kelkit Valley were genotyped using the spoligotyping method.   Results: Approximately 11.6% of the strains were resistant against at least one major drug, and 8.3% were resistant against more than one drug. Single-drug resistance for INH, ETB, SM, and RIF was found as 5.8%, 2.1%, 3.7%, and 0.0%, respectively. Two or more drug resistance rates for SM + ETB, SM + INH, INH + RIF, RIF + ETB, INH + ETB + RIF, SM + ETB + INH and INH + ETB + RIF + SM were determined as 0.8%, 1.7%, 0.4%, 0.8%, 2.9%, 0.4%, and 1.2%, respectively. Fifteen spoligotype groups were created when the results were compared with the world databank (SpoIDB4). Families of four strains could not be determined. The spoligotype groups most frequently encountered in our study were ST4 (n = 10, 20.8%), ST53 (n = 6, 12.5%), ST7 (n = 4, 8.3%), ST41 (n = 4, 8.3%) and ST31 (n = 4, 8.3%), and the most frequently encountered families were TI (n = 18, 37.5%), S (n = 12, 25%) and LAM-7 TUR (n = 4, 8.3%).   Conclusions: High rates of primary resistance against major anti-tuberculosis drugs, the LAM-7 TUR clone, and high grouping rates were detected in this study, the first such study carried out in the Kelkit Valley.

 

Aim: The aim of this study was to determine the primary drug resistance rates and genotypes of resistant Mycobacterium tuberculosis strains isolated in the Kelkit Valley.   Materials and Methods: Primary resistance to isoniazid (INH), rifampicin (RIF), ethambutol (ETB), and streptomycin (SM) was determined with the BACTEC 460 radiometric system. Forty-eight resistant M. tuberculosis strains isolated from tuberculosis patients living in the Kelkit Valley were genotyped using the spoligotyping method.   Results: Approximately 11.6% of the strains were resistant against at least one major drug, and 8.3% were resistant against more than one drug. Single-drug resistance for INH, ETB, SM, and RIF was found as 5.8%, 2.1%, 3.7%, and 0.0%, respectively. Two or more drug resistance rates for SM + ETB, SM + INH, INH + RIF, RIF + ETB, INH + ETB + RIF, SM + ETB + INH and INH + ETB + RIF + SM were determined as 0.8%, 1.7%, 0.4%, 0.8%, 2.9%, 0.4%, and 1.2%, respectively. Fifteen spoligotype groups were created when the results were compared with the world databank (SpoIDB4). Families of four strains could not be determined. The spoligotype groups most frequently encountered in our study were ST4 (n = 10, 20.8%), ST53 (n = 6, 12.5%), ST7 (n = 4, 8.3%), ST41 (n = 4, 8.3%) and ST31 (n = 4, 8.3%), and the most frequently encountered families were TI (n = 18, 37.5%), S (n = 12, 25%) and LAM-7 TUR (n = 4, 8.3%).   Conclusions: High rates of primary resistance against major anti-tuberculosis drugs, the LAM-7 TUR clone, and high grouping rates were detected in this study, the first such study carried out in the Kelkit Valley.

Makale detayları

Primary Drug Resistance and Molecular Epidemiology of the Mycobacterium tuberculosis Strains Isolated in the Kelkit Valley

Yazarlar:

Yunus BULUT | Gülgün YENİŞEHİRLİ | Barış OTLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2009 Vol.39 Issue 1, pp.101-107

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000755613

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

M. tuberculosis

primary drug resistance

molecular epidemiology

Sayı

Makale detayları

Primary Drug Resistance and Molecular Epidemiology of the Mycobacterium tuberculosis Strains Isolated in the Kelkit Valley

Yazarlar: Yunus BULUT | Gülgün YENİŞEHİRLİ | Barış OTLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2009 Vol.39 Issue 1, pp.101-107

Belge Tipi: Makale

Yayın Tarihi: 12/2008

Belge Dili: Türkçe

Document ID (Org No.): 2000755613

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

M. tuberculosis

primary drug resistance

molecular epidemiology

Sayı

Yazarlar:

Yunus BULUT | Gülgün YENİŞEHİRLİ | Barış OTLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2009 Vol.39 Issue 1, pp.101-107

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

Türkçe

Document ID (Org No.):

2000755613

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144