The protective effect of spironolactone and role of the Na+/K+- ATPase pump on intestinal ischemia/reperfusion injury

Cebrail Akyüz; Orhan Uzun; Oğuzhan Sunamak; Ahmet Özer Şehirli

Makale detayları

The protective effect of spironolactone and role of the Na+/K+- ATPase pump on intestinal ischemia/reperfusion injury

Yazarlar:

Cebrail Akyüz | Orhan Uzun | Oğuzhan Sunamak | Ahmet Özer Şehirli

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 3, pp.429-435

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346289

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The aim of this study was to evaluate the possible protective effect of spironolactone SPL and role of the Na-K ATPase pump on intestinal ischemiareperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone 20 mg kg-1 was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal NaK-ATPase, myeloperoxidase MPO analysis were performed. Structural injury was assessed histopathologically. Ischemiareperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemiareperfusion decreased NaK- ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemiareperfusion injury by different mechanisms, mainly the activity of the NaK-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

 The aim of this study was to evaluate the possible protective effect of spironolactone SPL and role of the Na-K ATPase pump on intestinal ischemiareperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone 20 mg kg-1 was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal NaK-ATPase, myeloperoxidase MPO analysis were performed. Structural injury was assessed histopathologically. Ischemiareperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemiareperfusion decreased NaK- ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemiareperfusion injury by different mechanisms, mainly the activity of the NaK-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

 The aim of this study was to evaluate the possible protective effect of spironolactone SPL and role of the Na-K ATPase pump on intestinal ischemiareperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone 20 mg kg-1 was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal NaK-ATPase, myeloperoxidase MPO analysis were performed. Structural injury was assessed histopathologically. Ischemiareperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemiareperfusion decreased NaK- ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemiareperfusion injury by different mechanisms, mainly the activity of the NaK-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

 The aim of this study was to evaluate the possible protective effect of spironolactone SPL and role of the Na-K ATPase pump on intestinal ischemiareperfusion injury. In our study, the period of ischemia was established by clamping the mesenteric artery for 45 minunder anesthesia in Wistar albino rats and the animals left for reperfusion at the end of this period were decapitated after one hour. Spironolactone 20 mg kg-1 was administered orally for three days before ischemia, 30 minbefore ischemia. The control group rats were subjected to the Sham operation and administered saline solution. TNF-α and IL-1β levels were measured in the serum samples. Ileal NaK-ATPase, myeloperoxidase MPO analysis were performed. Structural injury was assessed histopathologically. Ischemiareperfusion increased serum TNF-α and IL-1β levels together with MPO activity, whereas these values were maintained at the control group levels through SPL activation. However, ischemiareperfusion decreased NaK- ATPase activity in ileal tissues; however, these parameters were found to be significantly increased with SPL activation. The protective effect of SPL against ischemiareperfusion injury by different mechanisms, mainly the activity of the NaK-ATPase pump, suggests that this nontoxic agent may constitute a new clinical therapeutic principle.

Makale detayları

The protective effect of spironolactone and role of the Na+/K+- ATPase pump on intestinal ischemia/reperfusion injury

Yazarlar:

Cebrail Akyüz | Orhan Uzun | Oğuzhan Sunamak | Ahmet Özer Şehirli

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 3, pp.429-435

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346289

pISSN:

2630-6344

Intestinal ischemia/reperfusion

inflammation

Na+/K+-ATPase

spironolactone

Sayı

Makale detayları

The protective effect of spironolactone and role of the Na+/K+- ATPase pump on intestinal ischemia/reperfusion injury

Yazarlar: Cebrail Akyüz | Orhan Uzun | Oğuzhan Sunamak | Ahmet Özer Şehirli

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.22 Issue 3, pp.429-435

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346289

pISSN: 2630-6344

Intestinal ischemia/reperfusion

inflammation

Na+/K+-ATPase

spironolactone

Sayı

Yazarlar:

Cebrail Akyüz | Orhan Uzun | Oğuzhan Sunamak | Ahmet Özer Şehirli

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 3, pp.429-435

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346289

pISSN:

2630-6344