Poly(hexamethylene biguanide) immobilized non-absorbable and antimicrobial PET fiber for surgical suture applications: synthesis, characterization and in vitro cytocompatibility assessment

Ogün Bozkaya; Kübra Günay; Esra Bozkaya; Metin Arslan

Makale detayları

Poly(hexamethylene biguanide) immobilized non-absorbable and antimicrobial PET fiber for surgical suture applications: synthesis, characterization and in vitro cytocompatibility assessment

Yazarlar:

Ogün Bozkaya | Kübra Günay | Esra Bozkaya | Metin Arslan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Biomaterial

Dergi:

International Journal of Engineering Research and Development Vol.16 Issue 2, pp.777-790

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314853

pISSN:

1308-5506

Anahtar kelimeler:

Özet:

 Surgical sutures are widely utilized medical devices for the purpose of closing wounds after surgical interventions. During surgical procedures, sutures can come into contact with microorganisms in the surrounding environment, leading to the development of bacterial biofilms and subsequent surgical site infections SSI. Therefore, the use of sutures that prevent bacterial attachment and colonization is very important in wound healing. Antimicrobial sutures coated with antiseptics such as triclosan and chlorhexidine have been used to minimize SSI formation. However, due to the emergence of bacterial strains resistant to various antiseptics and antibiotics, new approaches to the development of alternative antimicrobial sutures are required. Among antimicrobial agents, polyhexanide polyhexamethylene biguanide, PHMB is a cationic antiseptic with broad spectrum antibacterial and antifungal activity, low toxicity and biocompatible. The aim of this study was to synthesize PHMB immobilized polyethylene terephthalate PET fibers for use as surgical suture threads. The synthesized modified PET fibers were characterized by SEM, FTIR, DSC and TGA. In addition, the wettability and in vitro degradation profile of the modified PET fibers were investigated. S. aureus and E. coli bacterial strains were used for antimicrobial activity tests. In vitro cytocompatibility cytotoxicity of the materials was evaluated by MTT test according to TS EN ISO 10993-5 standard.

 Surgical sutures are widely utilized medical devices for the purpose of closing wounds after surgical interventions. During surgical procedures, sutures can come into contact with microorganisms in the surrounding environment, leading to the development of bacterial biofilms and subsequent surgical site infections SSI. Therefore, the use of sutures that prevent bacterial attachment and colonization is very important in wound healing. Antimicrobial sutures coated with antiseptics such as triclosan and chlorhexidine have been used to minimize SSI formation. However, due to the emergence of bacterial strains resistant to various antiseptics and antibiotics, new approaches to the development of alternative antimicrobial sutures are required. Among antimicrobial agents, polyhexanide polyhexamethylene biguanide, PHMB is a cationic antiseptic with broad spectrum antibacterial and antifungal activity, low toxicity and biocompatible. The aim of this study was to synthesize PHMB immobilized polyethylene terephthalate PET fibers for use as surgical suture threads. The synthesized modified PET fibers were characterized by SEM, FTIR, DSC and TGA. In addition, the wettability and in vitro degradation profile of the modified PET fibers were investigated. S. aureus and E. coli bacterial strains were used for antimicrobial activity tests. In vitro cytocompatibility cytotoxicity of the materials was evaluated by MTT test according to TS EN ISO 10993-5 standard.

 Surgical sutures are widely utilized medical devices for the purpose of closing wounds after surgical interventions. During surgical procedures, sutures can come into contact with microorganisms in the surrounding environment, leading to the development of bacterial biofilms and subsequent surgical site infections SSI. Therefore, the use of sutures that prevent bacterial attachment and colonization is very important in wound healing. Antimicrobial sutures coated with antiseptics such as triclosan and chlorhexidine have been used to minimize SSI formation. However, due to the emergence of bacterial strains resistant to various antiseptics and antibiotics, new approaches to the development of alternative antimicrobial sutures are required. Among antimicrobial agents, polyhexanide polyhexamethylene biguanide, PHMB is a cationic antiseptic with broad spectrum antibacterial and antifungal activity, low toxicity and biocompatible. The aim of this study was to synthesize PHMB immobilized polyethylene terephthalate PET fibers for use as surgical suture threads. The synthesized modified PET fibers were characterized by SEM, FTIR, DSC and TGA. In addition, the wettability and in vitro degradation profile of the modified PET fibers were investigated. S. aureus and E. coli bacterial strains were used for antimicrobial activity tests. In vitro cytocompatibility cytotoxicity of the materials was evaluated by MTT test according to TS EN ISO 10993-5 standard.

 Cerrahi sütürler, cerrahi müdahalelerden sonra yaraları kapatmak amacıyla yaygın olarak kullanılan tıbbi cihazlardır. Cerrahi prosedürler sırasında, sütürler çevredeki mikroorganizmalarla temas ederek bakteriyel biyofilmlerin gelişmesine ve ardından cerrahi alan enfeksiyonlarına CAE yol açabilir. Bu nedenle bakteri tutunmasını ve kolonizasyonunu engelleyen sütürlerin kullanılması yara iyileşmesinde oldukça önemlidir. CAE oluşumunu en aza indirmek için triklosan ve klorheksidin gibi antiseptiklerle kaplı antimikrobiyal sütürler kullanılmıştır. Ancak çeşitli antiseptiklere ve antibiyotiklere dirençli bakteri suşlarının ortaya çıkması nedeniyle alternatif antimikrobiyal sütürlerin geliştirilmesine yönelik yeni yaklaşımlar gereklidir. Antimikrobiyal ajanlar arasında, poliheksanid poliheksametilen biguanid, PHMB geniş spektrumlu antibakteriyel ve antifungal aktiviteye sahip, düşük toksisiteli ve biyouyumlu katyonik bir antiseptiktir. Bu çalışmada cerrahi dikiş ipliği olarak kullanılmak üzere PHMB immobilize edilmiş polietilen tereftalat PET liflerin sentezlenmesi amaçlanmıştır. Sentezlenen modifiye PET lifler SEM, FTIR, DSC ve TGA ile karakterize edilmiştir. Bunlara ek olarak modifier PET liflerin ıslanabirliği ve in vitro degradasyon profili araştırılmıştır. Antimikrobiyal etkinlik testleri için S. aureus ve E. coli bakteri suşları kullanılmıştır. Materyallerin in vitro hücre-uyumluluğu sitotoksisitesi TS EN ISO 10993-5 standardına göre MTT testi ile değerlendirilmiştir.

Makale detayları

Poly(hexamethylene biguanide) immobilized non-absorbable and antimicrobial PET fiber for surgical suture applications: synthesis, characterization and in vitro cytocompatibility assessment

Yazarlar:

Ogün Bozkaya | Kübra Günay | Esra Bozkaya | Metin Arslan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Biomaterial

Dergi:

International Journal of Engineering Research and Development Vol.16 Issue 2, pp.777-790

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314853

pISSN:

1308-5506

Surgical suture

Antimicrobial suture

Non-adsorbable suture

Polyhexamethylene biguanide

Polyhexanide

Polyethylene terephthalateCerrahi sütür

Emilemez sütür”

Antimikrobiyal sütür

Polihegzametilen biguanid

Poliheksanid

Polietilen tereftalat

Sayı

Makale detayları

Yazarlar: Ogün Bozkaya | Kübra Günay | Esra Bozkaya | Metin Arslan

Yayıncı: Kirikkale University

Ana Başlıklar: Biomaterial

Dergi: International Journal of Engineering Research and Development Vol.16 Issue 2, pp.777-790

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314853

pISSN: 1308-5506

Sayı

Yazarlar:

Ogün Bozkaya | Kübra Günay | Esra Bozkaya | Metin Arslan

Yayıncı:

Kirikkale University

Ana Başlıklar:

Biomaterial

Dergi:

International Journal of Engineering Research and Development Vol.16 Issue 2, pp.777-790

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314853

pISSN:

1308-5506