DNA-Binding Properties of Nickle  II  Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

Ali ARSLANTAS; Mehmet Salih AGİRTAS

doi:https://doi.org/10.17350/HJSE19030000054

Makale detayları

DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

Yazarlar:

Ali ARSLANTAS | Mehmet Salih AGİRTAS

Yayıncı:

Hitit University

Dergi:

Hittite Journal of Science and Engineering 2017 Vol.4 Issue 2, pp.91-97

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000451783

Sıklık:

Üç Aylık

eISSN:

2148-4171

DOI:

https://doi.org/10.17350/HJSE19030000054

Özet:

 

I n this study, previously synthesized Ni  II  phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni  II  phthalocyanine compound. The DNA binding activities of Ni  II  phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni  II  phthalocyanine compound binds strongly to calf thymus-DNA via intercalation binding.

Makale detayları

DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

Yazarlar: Ali ARSLANTAS | Mehmet Salih AGİRTAS

Yayıncı: Hitit University

Dergi: Hittite Journal of Science and Engineering 2017 Vol.4 Issue 2, pp.91-97

Belge Tipi: Makale

Yayın Tarihi: 12/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000451783

Sıklık: Üç Aylık

eISSN: 2148-4171

DOI: https://doi.org/10.17350/HJSE19030000054

Sayı

Yazarlar:

Ali ARSLANTAS | Mehmet Salih AGİRTAS

Yayıncı:

Hitit University

Dergi:

Hittite Journal of Science and Engineering 2017 Vol.4 Issue 2, pp.91-97

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000451783

Sıklık:

Üç Aylık

eISSN:

2148-4171

DOI:

https://doi.org/10.17350/HJSE19030000054