Accumulation of Poly-b-Hydroxybutyrate in Streptomyces Species During Growth with Different Nitrogen Sources

Aysel UĞUR; Nurettin ŞAHİN

Makale detayları

Accumulation of Poly-b-Hydroxybutyrate in Streptomyces Species During Growth with Different Nitrogen Sources

Yazarlar:

Aysel UĞUR | Nurettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 3, pp.171-174

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813710

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The accumulation of poly-b-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between 0.3 and 7.6% of dry mycelial weight. PHB was produced by a selectant of Streptomyces isolates in media containing different nitrogen sources (KNO3, glycine, peptone, proteose peptone, L-asparagine, tryptone and malt extract), each combined with 2 g/l glucose as the carbon source. The most effective PHB production was observed on glycine/glucose medium with a 7.6% dry mycelial weight. Nitrogen limiting conditions were inhibitory to Streptomyces growth, but stimulated PHB accumulation. Based on the morphological and biochemical test results, selected isolates were identified as Streptomyces halstedii (MU112), S. anulatus (MU117) and S. rochei (MU119).

 

The accumulation of poly-b-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between 0.3 and 7.6% of dry mycelial weight. PHB was produced by a selectant of Streptomyces isolates in media containing different nitrogen sources (KNO3, glycine, peptone, proteose peptone, L-asparagine, tryptone and malt extract), each combined with 2 g/l glucose as the carbon source. The most effective PHB production was observed on glycine/glucose medium with a 7.6% dry mycelial weight. Nitrogen limiting conditions were inhibitory to Streptomyces growth, but stimulated PHB accumulation. Based on the morphological and biochemical test results, selected isolates were identified as Streptomyces halstedii (MU112), S. anulatus (MU117) and S. rochei (MU119).

Makale detayları

Accumulation of Poly-b-Hydroxybutyrate in Streptomyces Species During Growth with Different Nitrogen Sources

Yazarlar:

Aysel UĞUR | Nurettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 3, pp.171-174

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813710

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Poly-b-hydroxybutyric acid (PHB)

Streptomyces

nitrogen sources

Sayı

Makale detayları

Accumulation of Poly-b-Hydroxybutyrate in Streptomyces Species During Growth with Different Nitrogen Sources

Yazarlar: Aysel UĞUR | Nurettin ŞAHİN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2002 Vol.26 Issue 3, pp.171-174

Belge Tipi: Makale

Yayın Tarihi: 2/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000813710

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Poly-b-hydroxybutyric acid (PHB)

Streptomyces

nitrogen sources

Sayı

Yazarlar:

Aysel UĞUR | Nurettin ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 3, pp.171-174

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000813710

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152