Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates

Fatma AYHAN; Oguz AKPOLAT

doi:https://doi.org/10.32710/tekstilvekonfeksiyon.498774

Makale detayları

Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates

Yazarlar:

Fatma AYHAN | Oguz AKPOLAT

Yayıncı:

Ege University

Dergi:

Textile and Apparel 2021 Vol.31 Issue 1, pp.34-42

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000650573

Sıklık:

Üç Aylık

eISSN:

2602-3075

pISSN:

1300-3356

DOI:

https://doi.org/10.32710/tekstilvekonfeksiyon.498774

Özet:

 


The immobilization of
horseradish peroxidase in the form of cross-linked enzyme aggregates
(HRP–CLEAs) was optimized by three parameters full factorial experimental
design at two levels and the degradation of an azo dye was tested. The optimal
immobilization conditions were estimated as 0.06 mg enzyme/mL (0.96 U), 3 % (v/v,
Glutaraldehyde)) cross-linker ratio, and 6 mg /mL Bovine Serume Albumine amount,
respectively. The effects of variables were analysed by responce surface plots
and the enzyme concentration effect was insignificant for the tested levels. The
maximum CLEAs activity was 0.2188 U and the kinetic parameters (Km
and Vmax) were 0.0314 mM and 0.1044 mM/min while the calculated
values for soluble enzyme were 0.06 mM and 0.468 mM/min, respectively. Hydrogen
peroxide has inhibitory effect on enzyme aggregate activities at higher amounts
than 0.012 mM. Under optimal conditions, a textile azo dye, Reactive Blue 160
was completely oxidized within 90 h and higher temperatures also showed promising
results in dye degradation.  


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Makale detayları

Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates

Yazarlar: Fatma AYHAN | Oguz AKPOLAT

Yayıncı: Ege University

Dergi: Textile and Apparel 2021 Vol.31 Issue 1, pp.34-42

Belge Tipi: Makale

Yayın Tarihi: 3/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000650573

Sıklık: Üç Aylık

eISSN: 2602-3075

pISSN: 1300-3356

DOI: https://doi.org/10.32710/tekstilvekonfeksiyon.498774

Sayı

Yazarlar:

Fatma AYHAN | Oguz AKPOLAT

Yayıncı:

Ege University

Dergi:

Textile and Apparel 2021 Vol.31 Issue 1, pp.34-42

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000650573

Sıklık:

Üç Aylık

eISSN:

2602-3075

pISSN:

1300-3356

DOI:

https://doi.org/10.32710/tekstilvekonfeksiyon.498774