Neuroprotective role of n-acetylcysteine (NAC): countering doxorubicin neurotoxicity via TH, Nurr1, and iNOS expression

Tuğçe Anteplioğlu; Miyase Çınar; Gözde Yaldız; Sevgi Betül Kayabaşı; Özkan Duru; Ruhi Kabakçı

Makale detayları

Neuroprotective role of n-acetylcysteine (NAC): countering doxorubicin neurotoxicity via TH, Nurr1, and iNOS expression

Yazarlar:

Tuğçe Anteplioğlu | Miyase Çınar | Gözde Yaldız | Sevgi Betül Kayabaşı | Özkan Duru | Ruhi Kabakçı

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Pathology

Dergi:

Mediterranean Veterinary Journal Vol.9 Issue 2, pp.299-305

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326095

pISSN:

3023-8447

Anahtar kelimeler:

Özet:

 Chemotherapy is an effective treatment for cancer, but it can cause cognitive disorders broadly referred to as chemobrain. One of the most commonly used chemotherapeutics, doxorubicin DOX, has been associated with the potential for brain damage and cognitive dysfunction. N-acetylcysteine NAC has been identified as a potential brain protector with antiapoptotic, antioxidant, and anti-inflammatory effects. The objective of this study was to investigate the potential protective effect of NAC against DOX-induced brain damage. Female Wistar albino rats were randomly assigned to one of three groups: control, DOX, or NAC prophylaxis. Brain samples were collected for histopathological and immunohistochemical analyses, with a particular focus on regions that are crucial for cognition and memory. The DOX group exhibited significant histopathological changes, including neuronal shrinkage, degeneration, and necrosis in the striatum, hippocampal region, and cerebral cortex. Immunohistochemical analysis revealed the presence of neuroinflammation and neurodegeneration, with an increase in inducible nitric oxide synthetase iNOS immunopositivity. Administration of NAC effectively reduced iNOS immunopositivity, neuronal damage, degeneration, and necrosis in the prophylaxis group. Among the brain regions examined, the prophylaxis group demonstrated the most effective protection in the hippocampal region. Therefore, NAC has the potential to protect against or alleviate DOX-induced cognitive impairments.

 Chemotherapy is an effective treatment for cancer, but it can cause cognitive disorders broadly referred to as chemobrain. One of the most commonly used chemotherapeutics, doxorubicin DOX, has been associated with the potential for brain damage and cognitive dysfunction. N-acetylcysteine NAC has been identified as a potential brain protector with antiapoptotic, antioxidant, and anti-inflammatory effects. The objective of this study was to investigate the potential protective effect of NAC against DOX-induced brain damage. Female Wistar albino rats were randomly assigned to one of three groups: control, DOX, or NAC prophylaxis. Brain samples were collected for histopathological and immunohistochemical analyses, with a particular focus on regions that are crucial for cognition and memory. The DOX group exhibited significant histopathological changes, including neuronal shrinkage, degeneration, and necrosis in the striatum, hippocampal region, and cerebral cortex. Immunohistochemical analysis revealed the presence of neuroinflammation and neurodegeneration, with an increase in inducible nitric oxide synthetase iNOS immunopositivity. Administration of NAC effectively reduced iNOS immunopositivity, neuronal damage, degeneration, and necrosis in the prophylaxis group. Among the brain regions examined, the prophylaxis group demonstrated the most effective protection in the hippocampal region. Therefore, NAC has the potential to protect against or alleviate DOX-induced cognitive impairments.

 Chemotherapy is an effective treatment for cancer, but it can cause cognitive disorders broadly referred to as chemobrain. One of the most commonly used chemotherapeutics, doxorubicin DOX, has been associated with the potential for brain damage and cognitive dysfunction. N-acetylcysteine NAC has been identified as a potential brain protector with antiapoptotic, antioxidant, and anti-inflammatory effects. The objective of this study was to investigate the potential protective effect of NAC against DOX-induced brain damage. Female Wistar albino rats were randomly assigned to one of three groups: control, DOX, or NAC prophylaxis. Brain samples were collected for histopathological and immunohistochemical analyses, with a particular focus on regions that are crucial for cognition and memory. The DOX group exhibited significant histopathological changes, including neuronal shrinkage, degeneration, and necrosis in the striatum, hippocampal region, and cerebral cortex. Immunohistochemical analysis revealed the presence of neuroinflammation and neurodegeneration, with an increase in inducible nitric oxide synthetase iNOS immunopositivity. Administration of NAC effectively reduced iNOS immunopositivity, neuronal damage, degeneration, and necrosis in the prophylaxis group. Among the brain regions examined, the prophylaxis group demonstrated the most effective protection in the hippocampal region. Therefore, NAC has the potential to protect against or alleviate DOX-induced cognitive impairments.

 Chemotherapy is an effective treatment for cancer, but it can cause cognitive disorders broadly referred to as chemobrain. One of the most commonly used chemotherapeutics, doxorubicin DOX, has been associated with the potential for brain damage and cognitive dysfunction. N-acetylcysteine NAC has been identified as a potential brain protector with antiapoptotic, antioxidant, and anti-inflammatory effects. The objective of this study was to investigate the potential protective effect of NAC against DOX-induced brain damage. Female Wistar albino rats were randomly assigned to one of three groups: control, DOX, or NAC prophylaxis. Brain samples were collected for histopathological and immunohistochemical analyses, with a particular focus on regions that are crucial for cognition and memory. The DOX group exhibited significant histopathological changes, including neuronal shrinkage, degeneration, and necrosis in the striatum, hippocampal region, and cerebral cortex. Immunohistochemical analysis revealed the presence of neuroinflammation and neurodegeneration, with an increase in inducible nitric oxide synthetase iNOS immunopositivity. Administration of NAC effectively reduced iNOS immunopositivity, neuronal damage, degeneration, and necrosis in the prophylaxis group. Among the brain regions examined, the prophylaxis group demonstrated the most effective protection in the hippocampal region. Therefore, NAC has the potential to protect against or alleviate DOX-induced cognitive impairments.

Makale detayları

Neuroprotective role of n-acetylcysteine (NAC): countering doxorubicin neurotoxicity via TH, Nurr1, and iNOS expression

Yazarlar:

Tuğçe Anteplioğlu | Miyase Çınar | Gözde Yaldız | Sevgi Betül Kayabaşı | Özkan Duru | Ruhi Kabakçı

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Pathology

Dergi:

Mediterranean Veterinary Journal Vol.9 Issue 2, pp.299-305

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326095

pISSN:

3023-8447

doxorubicin

iNOS

N-acetylcysteine

Nurr1

tyrosine hydroxylase

Sayı

Makale detayları

Neuroprotective role of n-acetylcysteine (NAC): countering doxorubicin neurotoxicity via TH, Nurr1, and iNOS expression

Yazarlar: Tuğçe Anteplioğlu | Miyase Çınar | Gözde Yaldız | Sevgi Betül Kayabaşı | Özkan Duru | Ruhi Kabakçı

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Veterinary Pathology

Dergi: Mediterranean Veterinary Journal Vol.9 Issue 2, pp.299-305

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326095

pISSN: 3023-8447

doxorubicin

iNOS

N-acetylcysteine

Nurr1

tyrosine hydroxylase

Sayı

Yazarlar:

Tuğçe Anteplioğlu | Miyase Çınar | Gözde Yaldız | Sevgi Betül Kayabaşı | Özkan Duru | Ruhi Kabakçı

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Veterinary Pathology

Dergi:

Mediterranean Veterinary Journal Vol.9 Issue 2, pp.299-305

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326095

pISSN:

3023-8447