Determination of Effective Mutation Dose (ED50) To Be Used In Variety Treatment In Tea Plant

Burcu Göksu Karaoğlu; Yıldız Aka Kaçar; Kadriye Yaprak Kantoglu; Burak Kunter; Keziban Yazıcı

Makale detayları

Determination of Effective Mutation Dose (ED50) To Be Used In Variety Treatment In Tea Plant

Yazarlar:

Burcu Göksu Karaoğlu | Yıldız Aka Kaçar | Kadriye Yaprak Kantoglu | Burak Kunter | Keziban Yazıcı

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 2, pp.569-579

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318959

pISSN:

2687-2315

Anahtar kelimeler:

Özet:

 Although tea is cultivated only in the Eastern Black Sea region of Türkiye, all tea plantations in the area have been established from seeds since the beginning of tea farming. This has negatively affected both yield and quality. The high level of heterozygosity in tea plants and the presence of self-incompatibility mechanisms prolong breeding efforts. Mutation breeding is used as a fast and practical method to induce genetic variation. In leading tea-producing countries such as China, India, Kenya, and Sri Lanka, commercially important tea cultivars have been developed through mutation breeding. However, no such studies have been conducted in Türkiye. This study aimed to determine the effective mutation dose that can be used to induce variation in tea plants. The research was conducted between 2020 and 2021 at the Tea Research Greenhouse of the Faculty of Agriculture, Recep Tayyip Erdoğan University. The plant material used was 34-node shoots of the Zihni Derin tea cultivar. These shoots were irradiated with 0, 10, 20, 30, 40, 50, 60, and 70 Gray Gy doses using the Ob-Servo Sanguis Co-60 Research Irradiator gamma irradiation device at the Turkish Energy, Nuclear and Mineral Research Agency. Single-node cuttings obtained from the irradiated shoots were planted in a peat-vermiculite mixture. Survival and rooting rates of the tea cuttings exposed to different gamma doses were recorded, and shoot length mm was measured in the growing tea seedlings. Survival and rooting rates decreased with increasing radiation dose. Based on a probit regression analysis of shoot length, the Effective Mutation Dose for tea was determined to be 11.45 Gy. This study represents the first mutation breeding research on tea in Türkiye and is expected to provide a foundation for future work in this field.

 Although tea is cultivated only in the Eastern Black Sea region of Türkiye, all tea plantations in the area have been established from seeds since the beginning of tea farming. This has negatively affected both yield and quality. The high level of heterozygosity in tea plants and the presence of self-incompatibility mechanisms prolong breeding efforts. Mutation breeding is used as a fast and practical method to induce genetic variation. In leading tea-producing countries such as China, India, Kenya, and Sri Lanka, commercially important tea cultivars have been developed through mutation breeding. However, no such studies have been conducted in Türkiye. This study aimed to determine the effective mutation dose that can be used to induce variation in tea plants. The research was conducted between 2020 and 2021 at the Tea Research Greenhouse of the Faculty of Agriculture, Recep Tayyip Erdoğan University. The plant material used was 34-node shoots of the Zihni Derin tea cultivar. These shoots were irradiated with 0, 10, 20, 30, 40, 50, 60, and 70 Gray Gy doses using the Ob-Servo Sanguis Co-60 Research Irradiator gamma irradiation device at the Turkish Energy, Nuclear and Mineral Research Agency. Single-node cuttings obtained from the irradiated shoots were planted in a peat-vermiculite mixture. Survival and rooting rates of the tea cuttings exposed to different gamma doses were recorded, and shoot length mm was measured in the growing tea seedlings. Survival and rooting rates decreased with increasing radiation dose. Based on a probit regression analysis of shoot length, the Effective Mutation Dose for tea was determined to be 11.45 Gy. This study represents the first mutation breeding research on tea in Türkiye and is expected to provide a foundation for future work in this field.

 Although tea is cultivated only in the Eastern Black Sea region of Türkiye, all tea plantations in the area have been established from seeds since the beginning of tea farming. This has negatively affected both yield and quality. The high level of heterozygosity in tea plants and the presence of self-incompatibility mechanisms prolong breeding efforts. Mutation breeding is used as a fast and practical method to induce genetic variation. In leading tea-producing countries such as China, India, Kenya, and Sri Lanka, commercially important tea cultivars have been developed through mutation breeding. However, no such studies have been conducted in Türkiye. This study aimed to determine the effective mutation dose that can be used to induce variation in tea plants. The research was conducted between 2020 and 2021 at the Tea Research Greenhouse of the Faculty of Agriculture, Recep Tayyip Erdoğan University. The plant material used was 34-node shoots of the Zihni Derin tea cultivar. These shoots were irradiated with 0, 10, 20, 30, 40, 50, 60, and 70 Gray Gy doses using the Ob-Servo Sanguis Co-60 Research Irradiator gamma irradiation device at the Turkish Energy, Nuclear and Mineral Research Agency. Single-node cuttings obtained from the irradiated shoots were planted in a peat-vermiculite mixture. Survival and rooting rates of the tea cuttings exposed to different gamma doses were recorded, and shoot length mm was measured in the growing tea seedlings. Survival and rooting rates decreased with increasing radiation dose. Based on a probit regression analysis of shoot length, the Effective Mutation Dose for tea was determined to be 11.45 Gy. This study represents the first mutation breeding research on tea in Türkiye and is expected to provide a foundation for future work in this field.

 Çay bitkisi Türkiyede sadece Doğu Karadeniz bölgesinde yetiştirilmekle birlikte yetiştiriciliğinin bu bölgede yaygınlaşmaya başladığı tarihten itibaren bahçeler tohumla tesis edilmiştir. Bu durum verim ve kaliteyi olumsuz yönde etkilemektedir. Çay bitkisinde heterezigoti oranının yüksek olması ve kendine uyuşmazlık mekanizmasının varlığı ıslah çalışmalarının süresini uzatmaktadır. Hızlı ve pratik bir şekilde genetik çeşitlilik ortaya çıkarmak amacı ile mutasyon ıslahı yöntemi kullanılmaktadır. Çay üretim sıralamasında ilk sıralarda yer alan Çin, Hindistan, Kenya ve Sri Lanka gibi ülkelerde mutasyon ıslahı ile ticarete konu olan önemli çay çeşitleri geliştirilmiştir. Ülkemizde ise bu konuda bir çalışma yapılmamıştır. Bu çalışmada, çay bitkisinde varyasyon oluşturmada kullanılabilecek etkili mutasyon dozununun belirlenmesi amaçlanmıştır. Çalışmamız Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Çay Araştırma Serasında 2020-2021 yıllarında yürütülmüştür. Materyal olarak kullanılan Zihni Derin çay çeşidine ait 3-4 gözlü sürgünlere Türkiye Enerji Nükleer ve Maden Araştırma Kurumunda İzotop marka Ob-Servo Sanguis Co-60 Research Irradiator model gama ışınlama cihazı ile 0, 10, 20, 30, 40, 50, 60 ve 70 Gray Gy dozlarında ışınlama yapılarak tek gözlü olarak alınan çay çelikleri torf-vermikulit karışımına dikilmiştir. Farklı dozlarda gama ışını uygulanan çay çeliklerinin canlılık ve köklenme oranları belirlenmiş büyüyen çay fidanlarında ise sürgün uzunluğu mm ölçümleri yapılmıştır. Çay çeliklerinin canlılık ve köklenme oranları doz artışına bağlı olarak azalma göstermiştir. Sürgün uzunluğuna göre yapılan probit regresyon analizi sonucunda ise çayda Etkili Mutasyon Dozunun 11, 45 Gy olduğu tespit edilmiştir. Bu çalışma Türkiyede çayda mutasyon ıslahı konusunda yapılan ilk çalışma olup bundan sonraki çalışmalara ışık tutacak niteliğe sahiptir.

Makale detayları

Determination of Effective Mutation Dose (ED50) To Be Used In Variety Treatment In Tea Plant

Yazarlar:

Burcu Göksu Karaoğlu | Yıldız Aka Kaçar | Kadriye Yaprak Kantoglu | Burak Kunter | Keziban Yazıcı

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 2, pp.569-579

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318959

pISSN:

2687-2315

Mutation Breeding

Tea

Effective Dose

Gamma ray

Variety DevelopmentMutasyon Islahı

Çay

Etkili Doz

Gama Işını

Sayı

Makale detayları

Yazarlar: Burcu Göksu Karaoğlu | Yıldız Aka Kaçar | Kadriye Yaprak Kantoglu | Burak Kunter | Keziban Yazıcı

Yayıncı: Recep Tayyip Erdoğan University

Ana Başlıklar: Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi: Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 2, pp.569-579

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001318959

pISSN: 2687-2315

Sayı

Yazarlar:

Burcu Göksu Karaoğlu | Yıldız Aka Kaçar | Kadriye Yaprak Kantoglu | Burak Kunter | Keziban Yazıcı

Yayıncı:

Recep Tayyip Erdoğan University

Ana Başlıklar:

Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Dergi:

Recep Tayyip Erdoğan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi Vol.6 Issue 2, pp.569-579

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001318959

pISSN:

2687-2315