Developing Resistant/Tolerant Charleston Pepper Varieties to Tomato Spotted Wilt Virus (TSWV) Disease Using Existing Pepper Lines

Hüseyin Basım; Osman Kandil; Cansu Ökem

Makale detayları

Developing Resistant/Tolerant Charleston Pepper Varieties to Tomato Spotted Wilt Virus (TSWV) Disease Using Existing Pepper Lines

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.4 Issue 2, pp.12-20

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328525

pISSN:

2791-8807

Anahtar kelimeler:

Özet:

 Pepper is a vegetable that belongs to the Capsicum genus, which includes 25 species and is part of the Solanaceae family. Several abiotic and biotic stress factors can limit pepper yield in cultivation. One of the top ten viruses that limit pepper production worldwide and cause economic losses is tomato spotted wilt virus TSWV. The use of chemical control along with cultural practices against viral diseases may sometimes not be effective in controlling the disease. However, the use of resistant varieties is considered the most effective and eco-friendly method of control in the cultivation of peppers. In this study, PCR reactions were conducted using SCAC568 primers to evaluate the resistancetolerance or susceptibility of 27 different hybrid varieties of Charleston peppers grown under cover, as determined by our breeding program. The PCR products obtained were genotypically analyzed after being digested with XbaI. Based on the results, it was determined that out of the 27 Charleston pepper varieties studied, 66.6 were tolerant and heterozygous Rr, while 33.4 were susceptible. This study aims to develop TSWV-resistant Charleston pepper varieties.

 Pepper is a vegetable that belongs to the Capsicum genus, which includes 25 species and is part of the Solanaceae family. Several abiotic and biotic stress factors can limit pepper yield in cultivation. One of the top ten viruses that limit pepper production worldwide and cause economic losses is tomato spotted wilt virus TSWV. The use of chemical control along with cultural practices against viral diseases may sometimes not be effective in controlling the disease. However, the use of resistant varieties is considered the most effective and eco-friendly method of control in the cultivation of peppers. In this study, PCR reactions were conducted using SCAC568 primers to evaluate the resistancetolerance or susceptibility of 27 different hybrid varieties of Charleston peppers grown under cover, as determined by our breeding program. The PCR products obtained were genotypically analyzed after being digested with XbaI. Based on the results, it was determined that out of the 27 Charleston pepper varieties studied, 66.6 were tolerant and heterozygous Rr, while 33.4 were susceptible. This study aims to develop TSWV-resistant Charleston pepper varieties.

 Pepper is a vegetable that belongs to the Capsicum genus, which includes 25 species and is part of the Solanaceae family. Several abiotic and biotic stress factors can limit pepper yield in cultivation. One of the top ten viruses that limit pepper production worldwide and cause economic losses is tomato spotted wilt virus TSWV. The use of chemical control along with cultural practices against viral diseases may sometimes not be effective in controlling the disease. However, the use of resistant varieties is considered the most effective and eco-friendly method of control in the cultivation of peppers. In this study, PCR reactions were conducted using SCAC568 primers to evaluate the resistancetolerance or susceptibility of 27 different hybrid varieties of Charleston peppers grown under cover, as determined by our breeding program. The PCR products obtained were genotypically analyzed after being digested with XbaI. Based on the results, it was determined that out of the 27 Charleston pepper varieties studied, 66.6 were tolerant and heterozygous Rr, while 33.4 were susceptible. This study aims to develop TSWV-resistant Charleston pepper varieties.

 Biber, Solanaceae familyasına ait, 25 tür içeren Capsicum cinsine ait bir sebzedir. Biber yetiştiriciliğinde biber verimini sınırlayabilen hem abiyotik hem de biyotik çeşitli stres faktörleri bulunmaktadır. Dünya çapında biber üretimini sınırlayan ve ekonomik kayıplara neden olan ilk on virüsten biri domates benekli dolgunluk virüsüdür TSWV. Viral hastalıklara karşı kültürel uygulamalarla birlikte kimyasal mücadelenin kullanılması bazen hastalığın kontrolünde etkili olamayabilir. Ancak biber yetiştiriciliğinde dayanıklı çeşitlerin kullanılması en etkili ve çevre dostu mücadele yöntemi olarak kabul edilmektedir. Bu çalışmada, örtü altında yetiştirilen 27 farklı hibrit Charleston biber çeşidinin ıslah programımız tarafından belirlenen dirençtolerans veya duyarlılığını değerlendirmek için SCAC568 primerleri kullanılarak PCR reaksiyonları gerçekleştirildi. Elde edilen PCR ürünleri XbaI ile sindirildikten sonra genotipik olarak analiz edildi. Sonuçlara göre incelenen 27 Charleston biber çeşidinin 66,6sının toleranslı ve heterozigot Rr, 33,4ünün duyarlı olduğu belirlendi. Bu çalışmanın amacı, TSWVye dayanıklıtoleranslı Charleston biber çeşitlerinin geliştirilmesidir.

Makale detayları

Developing Resistant/Tolerant Charleston Pepper Varieties to Tomato Spotted Wilt Virus (TSWV) Disease Using Existing Pepper Lines

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.4 Issue 2, pp.12-20

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328525

pISSN:

2791-8807

Tomato spotted wilt virus (TSWV)

Charleston pepper

MAS

Tsw geneDomates Benekli Solgunluk Virüsü (TSWV)

Çarliston biber

MAS

Sayı

Makale detayları

Developing Resistant/Tolerant Charleston Pepper Varieties to Tomato Spotted Wilt Virus (TSWV) Disease Using Existing Pepper Lines

Yazarlar: Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı: Bingol University

Ana Başlıklar: Fitopatoloji

Dergi: Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.4 Issue 2, pp.12-20

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328525

pISSN: 2791-8807

Tomato spotted wilt virus (TSWV)

Charleston pepper

MAS

Tsw geneDomates Benekli Solgunluk Virüsü (TSWV)

Çarliston biber

MAS

Sayı

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.4 Issue 2, pp.12-20

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328525

pISSN:

2791-8807