CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin

Günseli Birge; Derya Koyuncu Zeybek

Makale detayları

CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin

Yazarlar:

Günseli Birge | Derya Koyuncu Zeybek

Yayıncı:

Gazi University

Ana Başlıklar:

Sensor Technology

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1674-1689

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331661

pISSN:

2147-1762

Anahtar kelimeler:

Özet:

 Herein, a label-free electrochemical leptin immunosensor was demonstrated. The sensing platform consists of the immobilizing of the anti-leptin antibody on a glassy carbon electrode GCE modified with cobalt iron oxide CoFe2O4 nanoparticles, chitosan CHI, and gold nanoparticles AuNPs. A simple and rapid leptin determination was achieved by measuring the change of current response in a redox probe solution before and after the immunocomplex formation. SEM examined the surface morphologies of the prepared electrodes. The electrochemical performance of the leptin immunosensor was commented on via EIS, CV, and DPV. Under optimized circumstances, a linear response was found between the current peaks acquired from DPV and the logarithm concentration of leptin in the range of 14000 ng mL-1 with a detection limit LOD of 0.1 ng mL-1. The subjected immunosensor demonstrated satisfactory reproducibility.

 Herein, a label-free electrochemical leptin immunosensor was demonstrated. The sensing platform consists of the immobilizing of the anti-leptin antibody on a glassy carbon electrode GCE modified with cobalt iron oxide CoFe2O4 nanoparticles, chitosan CHI, and gold nanoparticles AuNPs. A simple and rapid leptin determination was achieved by measuring the change of current response in a redox probe solution before and after the immunocomplex formation. SEM examined the surface morphologies of the prepared electrodes. The electrochemical performance of the leptin immunosensor was commented on via EIS, CV, and DPV. Under optimized circumstances, a linear response was found between the current peaks acquired from DPV and the logarithm concentration of leptin in the range of 14000 ng mL-1 with a detection limit LOD of 0.1 ng mL-1. The subjected immunosensor demonstrated satisfactory reproducibility.

 Herein, a label-free electrochemical leptin immunosensor was demonstrated. The sensing platform consists of the immobilizing of the anti-leptin antibody on a glassy carbon electrode GCE modified with cobalt iron oxide CoFe2O4 nanoparticles, chitosan CHI, and gold nanoparticles AuNPs. A simple and rapid leptin determination was achieved by measuring the change of current response in a redox probe solution before and after the immunocomplex formation. SEM examined the surface morphologies of the prepared electrodes. The electrochemical performance of the leptin immunosensor was commented on via EIS, CV, and DPV. Under optimized circumstances, a linear response was found between the current peaks acquired from DPV and the logarithm concentration of leptin in the range of 14000 ng mL-1 with a detection limit LOD of 0.1 ng mL-1. The subjected immunosensor demonstrated satisfactory reproducibility.

 Herein, a label-free electrochemical leptin immunosensor was demonstrated. The sensing platform consists of the immobilizing of the anti-leptin antibody on a glassy carbon electrode GCE modified with cobalt iron oxide CoFe2O4 nanoparticles, chitosan CHI, and gold nanoparticles AuNPs. A simple and rapid leptin determination was achieved by measuring the change of current response in a redox probe solution before and after the immunocomplex formation. SEM examined the surface morphologies of the prepared electrodes. The electrochemical performance of the leptin immunosensor was commented on via EIS, CV, and DPV. Under optimized circumstances, a linear response was found between the current peaks acquired from DPV and the logarithm concentration of leptin in the range of 14000 ng mL-1 with a detection limit LOD of 0.1 ng mL-1. The subjected immunosensor demonstrated satisfactory reproducibility.

Makale detayları

CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin

Yazarlar:

Günseli Birge | Derya Koyuncu Zeybek

Yayıncı:

Gazi University

Ana Başlıklar:

Sensor Technology

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1674-1689

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331661

pISSN:

2147-1762

Chitosan

Cobalt iron oxide

Gold nanoparticles

Immunosensor

Leptin

Sayı

Makale detayları

CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin

Yazarlar: Günseli Birge | Derya Koyuncu Zeybek

Yayıncı: Gazi University

Ana Başlıklar: Sensor Technology

Dergi: Gazi University Journal of Science Vol.37 Issue 4, pp.1674-1689

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001331661

pISSN: 2147-1762

Chitosan

Cobalt iron oxide

Gold nanoparticles

Immunosensor

Leptin

Sayı

Yazarlar:

Günseli Birge | Derya Koyuncu Zeybek

Yayıncı:

Gazi University

Ana Başlıklar:

Sensor Technology

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1674-1689

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331661

pISSN:

2147-1762