Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect   via enhanced glucose uptake and suppressed glucose absorption

Alaattin ŞEN; Buket AYAR; Anil YILMAZ; Özden Özgün ACAR; Gurbet Çelik TURGUT; Gülaçti TOPÇU

Makale detayları

Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption

Yazarlar:

Alaattin ŞEN | Buket AYAR | Anil YILMAZ | Özden Özgün ACAR | Gurbet Çelik TURGUT | Gülaçti TOPÇU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 5, pp.1350-1364

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725581

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Teucrium species have been used in folk medicine as antidiabetic,   antiinflammatory, antiulcer, and antibacterial agents. We have explored in   vitro antidiabetic impacts of 2 natural diterpenoids, alysine A and alysine   B, isolated from Teucrium alyssifolium. The lactate dehydrogenase (LDH) cytotoxicity assay,   glucose uptake test, glucose utilization (glycogen content) test, glucose   transport test, glucose absorption (αα\alpha -glucosidase activity) test,   insulin secretion test, RNA isolation and cDNA synthesis assay, qPCR   quantification assays, and statistical analyses were carried out in the   present study. Alysine A exerted the following effects at non-cytotoxic   doses:  . Enhanced the glucose uptake, as much as the insulin in the C2C12, HepG2, and 3T3-L1 cells  . Increased the glycogen content in the C2C12 and HepG2 liver cells, significantly higher than the insulin and metformin  . Suppressed the alpha-glucosidase and the GLUT2 expression levels in the Caco-2 cells  . Suppressed the SGLT1 and GLUT1-5 expression levels in the Caco-2 cells  . Induced the insulin receptor substrate (IRS)1 and GLUT2 expression levels of the BTC6 pancreatic cells   . Induced the insulin receptor (INSR), IRS2, phosphoinositide 3-kinase (PI3K), GLUT4, and protein kinase (PK) expression levels of the 3T3-L1 and C2C12 cells  .Increased glucose transport through the Caco-2 cell layer  . Did not influence insulin secretion in the pancreatic BTC6 cells  Consequently, these data strongly emphasized the antidiabetic action of   alysine A on the particularly critical model mechanisms that assume a part   in glucose homeostasis, such as glucose uptake, utilization, and storage.   Moreover, the expression level of the essential genes in glucose metabolism   and insulin signaling was altered in a way that the results would be   antihyperglycemic. A blend of in vitro and in situ tests affirmed the   antihyperglycemic action of alysine A and its mechanism. Alysine A has   exercised significant and positive results on the glucose homeostasis; thus,   it is a natural and pleiotropic antidiabetic agent. Advanced in vivo studies   are required to clarify the impact of this compound on glucose homeostasis   completely.

Makale detayları

Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption

Yazarlar: Alaattin ŞEN | Buket AYAR | Anil YILMAZ | Özden Özgün ACAR | Gurbet Çelik TURGUT | Gülaçti TOPÇU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 5, pp.1350-1364

Belge Tipi: Makale

Yayın Tarihi: 9/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000725581

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Alaattin ŞEN | Buket AYAR | Anil YILMAZ | Özden Özgün ACAR | Gurbet Çelik TURGUT | Gülaçti TOPÇU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 5, pp.1350-1364

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725581

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527