Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Cem EKMEKCİOGLU; Bettina ZODL; Susanne HUMPELER; Michaela ZEİNER; Claudia GUNDACKER; İlse STEFFAN

Makale detayları

Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Yazarlar:

Cem EKMEKCİOGLU | Bettina ZODL | Susanne HUMPELER | Michaela ZEİNER | Claudia GUNDACKER | İlse STEFFAN

Yayıncı:

Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi:

Cell Membranes and Free Radical Research 2010 Vol.2 Issue 2, pp.92-97

Belge Tipi:

Makale

Yayın Tarihi:

8/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000709023

Sıklık:

Üç Yıllık

eISSN:

2149-7222

Özet:

 

Aim of the presented study was to investigate whether scorbic acid enhances iron uptake and/or toxicity in Caco-2 cells. Cells were incubated with 1.5 mM of different iron species combined with increasing doses of ascorbic acid (1-3 mM). Thereafter viability, membrane damage, lipid peroxidation, and intracellular iron concentrations were measured. No effects on iron-induced damage to cells were found regarding viability. 
Lipid peroxidation significantly increased when ascorbic acid was added to cells incubated with FeSO. In 4 contrast lactate dehydrogenase (LDH)-release decreased by addition of ascorbic acid in Caco-2 cells treated with FeCl : nitrilotriacetic acid (NTA) and FeCl : citric acid. Iron uptake was enhanced by ascorbic acid, when 3 3
FeCl : citric acid (1:2) or FeSO  were used. 

We suggested that, especially dependent on the iron species used, 3 4 a combination of ascorbic acid and iron favours lipid peroxidation in Caco-2 cells. But on the other hand we also found beneficial effects of this type of co-treatment, like an enhanced iron uptake and decreased plasma membrane damage.

Makale detayları

Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Yazarlar: Cem EKMEKCİOGLU | Bettina ZODL | Susanne HUMPELER | Michaela ZEİNER | Claudia GUNDACKER | İlse STEFFAN

Yayıncı: Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi: Cell Membranes and Free Radical Research 2010 Vol.2 Issue 2, pp.92-97

Belge Tipi: Makale

Yayın Tarihi: 8/2013

Belge Dili: İngilizce

Document ID (Org No.): 2000709023

Sıklık: Üç Yıllık

eISSN: 2149-7222

Sayı

Yazarlar:

Cem EKMEKCİOGLU | Bettina ZODL | Susanne HUMPELER | Michaela ZEİNER | Claudia GUNDACKER | İlse STEFFAN

Yayıncı:

Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi:

Cell Membranes and Free Radical Research 2010 Vol.2 Issue 2, pp.92-97

Belge Tipi:

Makale

Yayın Tarihi:

8/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000709023

Sıklık:

Üç Yıllık

eISSN:

2149-7222