A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC

Tuğba Doğan; Tamay Şeker

Makale detayları

A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC

Yazarlar:

Tuğba Doğan | Tamay Şeker

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293128

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index RI detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision generally accepted as repeatability and reproducibility for the standard test method. The recovery values of the sugars added into juice sample were 92, 99 and 96 for sucrose, glucose and fructose, respectively.

 This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index RI detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision generally accepted as repeatability and reproducibility for the standard test method. The recovery values of the sugars added into juice sample were 92, 99 and 96 for sucrose, glucose and fructose, respectively.

 This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index RI detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision generally accepted as repeatability and reproducibility for the standard test method. The recovery values of the sugars added into juice sample were 92, 99 and 96 for sucrose, glucose and fructose, respectively.

 Bu çalışmada, TS EN 12630 standart metodunun doğrulanması ve piyasada bulunan meyve sularının şeker içeriklerinin yüksek basınçlı sıvı kromatografisi ile belirlenmesi amaçlanmıştır. Laboratuvar söz konusu standart test metodundaki performansını göstermek için, standart metodun parametrelerini teyit etmelidir. Bu amaçla piyasadan temin edilen portakal sularında Sükroz, Glikoz ve Fruktoz analizi yapılmıştır. Metodun prensibi, şekerlerin hareketli faz yardımı ile izokratik elüsyon ile bir katyon-değiştirici reçine üzerinde ayrılması, diferansiyel kırılma indisi RI dedektörü ve dış standart metodu kullanılarak saptanması esasına dayanmaktadır. Gün içi ve günler arası analizler ile yöntemin doğruluk ve kesinliği belirlenerek metot verifikasyonu gerçekleştirilmiştir. Meyve suyu örneğine eklenen şekerlerin geri kazanım değerleri sukroz, glikoz ve fruktoz için sırasıyla 92, 99 ve 96 bulunmuştur.

Makale detayları

A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC

Yazarlar:

Tuğba Doğan | Tamay Şeker

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293128

pISSN:

2687-475X

chromatography

sugar analysis

HPLC

Packed fruit juiceKromatografi

Şeker Analizi

HPLC

paketli meyve suyu

Sayı

Makale detayları

Yazarlar: Tuğba Doğan | Tamay Şeker

Yayıncı: Hacettepe University

Ana Başlıklar: Engineering

Dergi: Hacettepe Journal of Biology and Chemistry Vol.51 Issue 3, pp.289-295

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293128

pISSN: 2687-475X

Sayı

Yazarlar:

Tuğba Doğan | Tamay Şeker

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 3, pp.289-295

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293128

pISSN:

2687-475X