Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia

Teshome Biru; Aman Dekebo

Makale detayları

Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia

Yazarlar:

Teshome Biru | Aman Dekebo

Yayıncı:

İzzet KARA

Ana Başlıklar:

Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 3, pp.494-506

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322786

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis 500 g was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mgmL to 200 mgmL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.331.52 mm, Escherichia coli 14.331.53 mm and Salmonella typhimurium 16.001.00 mm, respectively. MeOH extract 1000 µgmL showed superior radical scavenging property 0.02 than ascorbic acid 0.05. The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4  of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance.

 Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis 500 g was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mgmL to 200 mgmL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.331.52 mm, Escherichia coli 14.331.53 mm and Salmonella typhimurium 16.001.00 mm, respectively. MeOH extract 1000 µgmL showed superior radical scavenging property 0.02 than ascorbic acid 0.05. The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4  of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance.

 Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis 500 g was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mgmL to 200 mgmL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.331.52 mm, Escherichia coli 14.331.53 mm and Salmonella typhimurium 16.001.00 mm, respectively. MeOH extract 1000 µgmL showed superior radical scavenging property 0.02 than ascorbic acid 0.05. The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4  of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance.

 Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis 500 g was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mgmL to 200 mgmL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.331.52 mm, Escherichia coli 14.331.53 mm and Salmonella typhimurium 16.001.00 mm, respectively. MeOH extract 1000 µgmL showed superior radical scavenging property 0.02 than ascorbic acid 0.05. The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4  of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance.

Makale detayları

Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia

Yazarlar:

Teshome Biru | Aman Dekebo

Yayıncı:

İzzet KARA

Ana Başlıklar:

Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 3, pp.494-506

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322786

pISSN:

2148-6905

Antimicrobial Antioxidant

Bay leaf

Essential oilAntimicrobial Antioxidant

Bay leaf

Essential oil

Sayı

Makale detayları

Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia

Yazarlar: Teshome Biru | Aman Dekebo

Yayıncı: İzzet KARA

Ana Başlıklar: Natural Products and Bioactive Compounds

Dergi: International Journal of Secondary Metabolite Vol.11 Issue 3, pp.494-506

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001322786

pISSN: 2148-6905

Antimicrobial Antioxidant

Bay leaf

Essential oilAntimicrobial Antioxidant

Bay leaf

Essential oil

Sayı

Yazarlar:

Teshome Biru | Aman Dekebo

Yayıncı:

İzzet KARA

Ana Başlıklar:

Natural Products and Bioactive Compounds

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 3, pp.494-506

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001322786

pISSN:

2148-6905