Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Derya KIZILOLUK; Gültekin GÖKÇE; Şenay AKKUŞ ÇETİNUS

Makale detayları

Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Yazarlar:

Derya KIZILOLUK | Gültekin GÖKÇE | Şenay AKKUŞ ÇETİNUS

Ana Başlıklar:

Kimya ve ilgili bilimler | Farmakoloji ve terapötik

Dergi:

Cumhuriyet Science Journal 2021 Vol.42 Issue 4, pp.814-821

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000920245

Özet:

 

In recent years, studies with biosensors have increased in order to better understand the mechanisms of anticancer drug action. Thus, studies to examine interactions with DNA using biosensors have gained momentum. In our study, it was investigated that Capecitabine (CPT), an anticancer drug, and glassy carbon electrode (GCE) interaction by using electrochemical methods. The interaction of CPT with calf thymus DNAs (dsDNA, ssDNA) immobilized on the electrode surface was analyzed by exploiting changes in the oxidation signals of the guanine base. The immobilization of DNA on the electrode surface has been optimized. Optimal DNA concentration and optimal interaction times were found. Electrochemical impedance spectroscopy technique was used for impedimetric measurements.The results obtained confirmed that the ct-DNAs were immobilized on the electrode surface. Detection limit (DL) was found as 17.54 μg / mL for interaction capecitabine with ds-DNA and was found 17.12 μg / mL for interaction capecitabine with ss-DNA.

Makale detayları

Electrochemical investigation of DNA and Capecitabine interaction using glassy carbon electrode (GCE)

Yazarlar: Derya KIZILOLUK | Gültekin GÖKÇE | Şenay AKKUŞ ÇETİNUS

Ana Başlıklar: Kimya ve ilgili bilimler | Farmakoloji ve terapötik

Dergi: Cumhuriyet Science Journal 2021 Vol.42 Issue 4, pp.814-821

Belge Tipi: Makale

Yayın Tarihi: 12/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000920245

Sayı

Yazarlar:

Derya KIZILOLUK | Gültekin GÖKÇE | Şenay AKKUŞ ÇETİNUS

Ana Başlıklar:

Kimya ve ilgili bilimler | Farmakoloji ve terapötik

Dergi:

Cumhuriyet Science Journal 2021 Vol.42 Issue 4, pp.814-821

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000920245