Chemical Composition and Antifungal Activity of Origanum acutidens Essential Oil Against Sclerotinia sclerotiorum (Lib.) de Bary and Phytophthora infestans (Mont.) de Bary

Yusuf Bayar; Zeynep Akşit; Samed Şimşek; Ali Kandemir

Makale detayları

Chemical Composition and Antifungal Activity of Origanum acutidens Essential Oil Against Sclerotinia sclerotiorum (Lib.) de Bary and Phytophthora infestans (Mont.) de Bary

Yazarlar:

Yusuf Bayar | Zeynep Akşit | Samed Şimşek | Ali Kandemir

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 5, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294132

pISSN:

2618-6578

Anahtar kelimeler:

Özet:

 The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry GC-MS analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol 4.76, p-cymene 7.6, linalool 14.82, and carvacrol 49.4. The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µLPetri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values lethal concentration at which 50 of the pathogens growth is inhibited for P. infestans and S. sclerotiorum were calculated as 0.982 µLPetri and 1.61 µLPetri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.

 The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry GC-MS analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol 4.76, p-cymene 7.6, linalool 14.82, and carvacrol 49.4. The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µLPetri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values lethal concentration at which 50 of the pathogens growth is inhibited for P. infestans and S. sclerotiorum were calculated as 0.982 µLPetri and 1.61 µLPetri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.

 The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry GC-MS analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol 4.76, p-cymene 7.6, linalool 14.82, and carvacrol 49.4. The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µLPetri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values lethal concentration at which 50 of the pathogens growth is inhibited for P. infestans and S. sclerotiorum were calculated as 0.982 µLPetri and 1.61 µLPetri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.

 The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry GC-MS analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol 4.76, p-cymene 7.6, linalool 14.82, and carvacrol 49.4. The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µLPetri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values lethal concentration at which 50 of the pathogens growth is inhibited for P. infestans and S. sclerotiorum were calculated as 0.982 µLPetri and 1.61 µLPetri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.

Makale detayları

Chemical Composition and Antifungal Activity of Origanum acutidens Essential Oil Against Sclerotinia sclerotiorum (Lib.) de Bary and Phytophthora infestans (Mont.) de Bary

Yazarlar:

Yusuf Bayar | Zeynep Akşit | Samed Şimşek | Ali Kandemir

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 5, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294132

pISSN:

2618-6578

Origanum acutidens

Essential oil

Antifungal activity

Plant pathogens

Sayı

Makale detayları

Chemical Composition and Antifungal Activity of Origanum acutidens Essential Oil Against Sclerotinia sclerotiorum (Lib.) de Bary and Phytophthora infestans (Mont.) de Bary

Yazarlar: Yusuf Bayar | Zeynep Akşit | Samed Şimşek | Ali Kandemir

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Ziraat Mühendisliği

Dergi: Black Sea Journal of Agriculture Vol.6 Issue 5, pp.439-443

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001294132

pISSN: 2618-6578

Origanum acutidens

Essential oil

Antifungal activity

Plant pathogens

Sayı

Yazarlar:

Yusuf Bayar | Zeynep Akşit | Samed Şimşek | Ali Kandemir

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 5, pp.439-443

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001294132

pISSN:

2618-6578