IRBESARTAN REDUCES LIVER DAMAGE INDUCED BY LIPOPOLYSACCHARIDE VIA INHIBITION OF TOTAL OXIDANT STATUS, INTERLEUKIN-1B AND CASPASE-3 LEVELS

Esra Nurlu Temel; İlter İlhan

Makale detayları

IRBESARTAN REDUCES LIVER DAMAGE INDUCED BY LIPOPOLYSACCHARIDE VIA INHIBITION OF TOTAL OXIDANT STATUS, INTERLEUKIN-1B AND CASPASE-3 LEVELS

Yazarlar:

Esra Nurlu Temel | İlter İlhan

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Infectious Diseases

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.474-483

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299118

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective In septic conditions, hyperinflammatory response and hepatotoxicity are caused by oxidative stress, inflammation, and apoptosis. Irbesartan IB, an adrenergic receptor blocker, has anti-inflammatory and antioxidant properties. This study aimed to investigate the protective effect of IB on lipopolysaccharide LPS- induced acute hepatotoxicity. Material and Method A total of eight rats were used in three groups; a control group; LPS group 5 mgkg, intraperitoneally IP; and LPS  IB group 5 mgkg LPS IP  50 mgkg IB orally. After sacrification, tissues from the liver and blood were obtained for immunohistochemical and biochemical evaluations, such as interleukin-1 beta IL-1β, caspase-3 Cas-3 alanine aminotransferase ALT, aspartate aminotransferase AST, oxidative stress index OSI, total oxidant status TOS, and total antioxidant status TAS. Results Compared with the control group, increased AST and ALT levels in the blood, biochemically increased TOS and OSI and decreased TAS levels in the tissue, immunohistochemically increased IL-1β, Cas- 3, detected. Also, in liver tissue, histopathologically hyperemia, hemorrhage, vacuolization, and significant neutrophilia infiltration were found in the LPS group. IB administration significantly reversed all these parameters. TAS levels were increased by IB administration, whereas TOS and OSI levels were decreased p  0.001. IB also decreased AST and ALT values p  0.001. In the IB group, Cas-3 and IL-1β levels were significantly decreased by IB administration p  0.001. In addition, the IB ameliorated histopathological findings showed enhanced hyperaemia, haemorrhages, vacuolisation and significant neutrophilic leukocyte infiltration p  0.001. IB treatment attenuated LPS-induced hepatotoxicity by its antioxidant, anti-inflammatory and antiapoptotic properties. Conclusion Attenuating liver injury and restoring liver function lowers morbidity and mortality rates in patients with sepsis. IB protects liver tissue from hepatotoxicity caused by LPS thanks to its antioxidant, anti-inflammatory, and anti-apoptotic properties. Further investigation of the livers role in sepsis may lead to the development of new therapeutic targets and strategies. IB may be an alternative therapeutic agent for the prevention of acute hepatotoxicity during sepsis.

 Objective In septic conditions, hyperinflammatory response and hepatotoxicity are caused by oxidative stress, inflammation, and apoptosis. Irbesartan IB, an adrenergic receptor blocker, has anti-inflammatory and antioxidant properties. This study aimed to investigate the protective effect of IB on lipopolysaccharide LPS- induced acute hepatotoxicity. Material and Method A total of eight rats were used in three groups; a control group; LPS group 5 mgkg, intraperitoneally IP; and LPS  IB group 5 mgkg LPS IP  50 mgkg IB orally. After sacrification, tissues from the liver and blood were obtained for immunohistochemical and biochemical evaluations, such as interleukin-1 beta IL-1β, caspase-3 Cas-3 alanine aminotransferase ALT, aspartate aminotransferase AST, oxidative stress index OSI, total oxidant status TOS, and total antioxidant status TAS. Results Compared with the control group, increased AST and ALT levels in the blood, biochemically increased TOS and OSI and decreased TAS levels in the tissue, immunohistochemically increased IL-1β, Cas- 3, detected. Also, in liver tissue, histopathologically hyperemia, hemorrhage, vacuolization, and significant neutrophilia infiltration were found in the LPS group. IB administration significantly reversed all these parameters. TAS levels were increased by IB administration, whereas TOS and OSI levels were decreased p  0.001. IB also decreased AST and ALT values p  0.001. In the IB group, Cas-3 and IL-1β levels were significantly decreased by IB administration p  0.001. In addition, the IB ameliorated histopathological findings showed enhanced hyperaemia, haemorrhages, vacuolisation and significant neutrophilic leukocyte infiltration p  0.001. IB treatment attenuated LPS-induced hepatotoxicity by its antioxidant, anti-inflammatory and antiapoptotic properties. Conclusion Attenuating liver injury and restoring liver function lowers morbidity and mortality rates in patients with sepsis. IB protects liver tissue from hepatotoxicity caused by LPS thanks to its antioxidant, anti-inflammatory, and anti-apoptotic properties. Further investigation of the livers role in sepsis may lead to the development of new therapeutic targets and strategies. IB may be an alternative therapeutic agent for the prevention of acute hepatotoxicity during sepsis.

 Objective In septic conditions, hyperinflammatory response and hepatotoxicity are caused by oxidative stress, inflammation, and apoptosis. Irbesartan IB, an adrenergic receptor blocker, has anti-inflammatory and antioxidant properties. This study aimed to investigate the protective effect of IB on lipopolysaccharide LPS- induced acute hepatotoxicity. Material and Method A total of eight rats were used in three groups; a control group; LPS group 5 mgkg, intraperitoneally IP; and LPS  IB group 5 mgkg LPS IP  50 mgkg IB orally. After sacrification, tissues from the liver and blood were obtained for immunohistochemical and biochemical evaluations, such as interleukin-1 beta IL-1β, caspase-3 Cas-3 alanine aminotransferase ALT, aspartate aminotransferase AST, oxidative stress index OSI, total oxidant status TOS, and total antioxidant status TAS. Results Compared with the control group, increased AST and ALT levels in the blood, biochemically increased TOS and OSI and decreased TAS levels in the tissue, immunohistochemically increased IL-1β, Cas- 3, detected. Also, in liver tissue, histopathologically hyperemia, hemorrhage, vacuolization, and significant neutrophilia infiltration were found in the LPS group. IB administration significantly reversed all these parameters. TAS levels were increased by IB administration, whereas TOS and OSI levels were decreased p  0.001. IB also decreased AST and ALT values p  0.001. In the IB group, Cas-3 and IL-1β levels were significantly decreased by IB administration p  0.001. In addition, the IB ameliorated histopathological findings showed enhanced hyperaemia, haemorrhages, vacuolisation and significant neutrophilic leukocyte infiltration p  0.001. IB treatment attenuated LPS-induced hepatotoxicity by its antioxidant, anti-inflammatory and antiapoptotic properties. Conclusion Attenuating liver injury and restoring liver function lowers morbidity and mortality rates in patients with sepsis. IB protects liver tissue from hepatotoxicity caused by LPS thanks to its antioxidant, anti-inflammatory, and anti-apoptotic properties. Further investigation of the livers role in sepsis may lead to the development of new therapeutic targets and strategies. IB may be an alternative therapeutic agent for the prevention of acute hepatotoxicity during sepsis.

 Amaç Septik koşullarda hiperinflamatuar yanıta ve hepatotoksisiteye; oksidatif stres, inflamasyon ve apoptoz neden olur. Bir adrenerjik reseptör blokeri olan irbesartan IB, antiinflamatuar ve antioksidan özelliklere sahiptir. Bu çalışmada, IBnin lipopolisakkarit LPS kaynaklı akut hepatotoksisite üzerindeki koruyucu etkisinin araştırılması amaçlandı. Gereç ve Yöntem Üç grupta toplam sekiz sıçan kullanıldı; kontrol grubu, LPS grubu 5 mgkg, intraperitoneal IP; ve LPS  IB grubu 5 mgkg LPS IP  50 mgkg IB oral. Sakrifikasyondan sonra interlökin-1 beta IL-1β, kaspaz- 3 Cas-3 alanin aminotransferaz ALT, aspartat aminotransferaz AST, oksidatif stres indeksi OSI, toplam oksidan durumu TOS ve toplam antioksidan durumu TAS gibi immünohistokimyasal ve biyokimyasal değerlendirmeler için karaciğer ve kandan dokular alındı. Bulgular Kontrol grubuyla karşılaştırıldığında kanda AST ve ALT düzeylerinde artış, biyokimyasal olarak dokuda TOS ve OSI düzeyinde artış ve TAS düzeyinde azalma, immünohistokimyasal olarak IL-1β, Cas-3 düzeyinde artış tespit edildi. Ayrıca LPS grubunda karaciğer dokusunda histopatolojik olarak hiperemi, kanama, vakuolizasyon ve belirgin nötrofil infiltrasyonu saptandı. IB tüm bu bulguları tersine çevirdi. IB uygulaması ile TAS seviyeleri yükselirken, TOS ve OSI seviyeleri azaldı p  0.001. IB ayrıca AST ve ALT değerlerini de düşürdü p  0.001. IB grubunda, Cas-3 ve IL-1β seviyeleri, IB uygulamasıyla önemli ölçüde azaldı p  0.001. Ek olarak, IB, artmış hiperemi, kanama, vakuolizasyon ve önemli nötrofilik lökosit infiltrasyon gibi histopatolojik bulguları iyileştirdi p  0.001. IB tedavisi, antioksidan, antienflamatuar ve antiapoptotik özellikleriyle LPSnin neden olduğu hepatotoksisiteyi zayıflattı. Sonuç Karaciğer hasarını hafifletmek ve karaciğer fonksiyonunu eski haline getirmek sepsisli hastalarda morbi- dite ve mortalite oranlarını düşürür. IB, antioksidan, antiinflamatuar ve antiapoptotik özellikleri sayesinde karaciğer dokusunu LPSnin neden olduğu hepatotoksisiteden korur. Karaciğerin sepsisteki rolünün daha fazla araştırılması, yeni terapötik hedeflerin ve stratejilerin geliştirilmesine yol açabilir. IB, sepsis sırasında akut hepatotoksisitenin önlenmesi için alternatif bir terapötik ajan olabilir.

Makale detayları

IRBESARTAN REDUCES LIVER DAMAGE INDUCED BY LIPOPOLYSACCHARIDE VIA INHIBITION OF TOTAL OXIDANT STATUS, INTERLEUKIN-1B AND CASPASE-3 LEVELS

Yazarlar:

Esra Nurlu Temel | İlter İlhan

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Infectious Diseases

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.474-483

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299118

pISSN:

1300-7416

Apoptosis

Hepatotoxicity

Irbesartan

Inflammation

Oxidative stress

SepsisSepsis

irbesartan

inflamasyon

hepatotoksisite

oksidatif stres

apoptoz

Sayı

Makale detayları

Yazarlar: Esra Nurlu Temel | İlter İlhan

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Infectious Diseases

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.474-483

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299118

pISSN: 1300-7416

Sayı

Yazarlar:

Esra Nurlu Temel | İlter İlhan

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Infectious Diseases

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.474-483

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299118

pISSN:

1300-7416