Evaluation of lung toxicity in rats kept in coal mine ambience by in vivo respiration records: An Experimental Study

Saliha Ayşenur ÇAM; Seyfullah Oktay ARSLAN

doi:https://doi.org/10.30569/adiyamansaglik.596944

Makale detayları

Evaluation of lung toxicity in rats kept in coal mine ambience by in vivo respiration records: An Experimental Study

Yazarlar:

Saliha Ayşenur ÇAM | Seyfullah Oktay ARSLAN

Yayıncı:

Adıyaman University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Health Sciences Journal of Adıyaman University 2019 Vol.5 Issue 2, pp.1500-1510

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000441109

Sıklık:

Üç Yıllık

eISSN:

2458-9179

DOI:

https://doi.org/10.30569/adiyamansaglik.596944

Özet:

 


OBJECTIVE: Mine
workers exposed to dusts or toxic gasses in occupational atmospheric
conditions. Pneumoconiosis and other lung diseases are charecterized by the
pathogenesis of coal dust-caused pulmonary toxicity associated with reactive
oxygen species (ROS).


 MATERIALS AND METHODS:
This study was conducted to
investigate the respiration failures and fibrosis in rats after being exposed
to coal dust and gases in mine atmosphere. Another aim was to study the therapeutic
effect of erdosteine as antioxidant therapy. Rats were exposed to mine ambience
for four week, and then they were breathed in the clean air for four week. The
respiratory functions of rats were recorded once a week for eight week, as in
vivo. The fibrosis of lung tissue levels, the oxidant/antioxidant status, and
cytokines of inflammation in bronchoalveolar lavage fluids (BALFs) were
evaluated, at the end of the processes.


RESULTS: We observed to be the beginning of respiratory
abnormalities in animals exposed to coal dust in second week. The end of fourth
week, there were the increase of respiratory frequency and along with the
decrease of respiratory depth. The respiratory failures were not improved in
clean ambience, moreover apnea were appearance in the end of six week (the
second week of clean air). Deaths were 28% in animals. Erdosteine administer to
rats could not fully abolished to the pulmonary toxicity, however could able to
hold to toxicity, and also there were not dies in rats administered to
erdosteine. Coal dust exposure was resulted in fibrosis with higher hydroxyproline
(HP) levels, cytokine inflammation with higher interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)
levels, and lipid peroxidation with an increased malondialdehyde (MDA) levels, according
to the healthy. A dramatically run out of endogen antidote sulfide pools (GSH),
and an increased MPO activity were dedected in the mine dusts and gasses
exposure group, according to the healthy animals. High biochemical index of
toxicity were partly balanced by erdosteine.









CONCLUSIONS: Our experimental findings support the hypothesis
that ROS is induced coal workers' pneumoconiosis. Re-oxygenation cannot be getting
it together to reverse the pulmonary toxicity. On the top of it, it can make
its pathogenesis further exaggerating and even worse. On this account we heartily
speculate that re-oxygenation should be by steps in mine workers. In addition
the antioxidant therapy may be partly a choice be able to tolerate the coal dust-induced
lung toxicity of mine workers.


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Makale detayları

Evaluation of lung toxicity in rats kept in coal mine ambience by in vivo respiration records: An Experimental Study

Yazarlar: Saliha Ayşenur ÇAM | Seyfullah Oktay ARSLAN

Yayıncı: Adıyaman University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Health Sciences Journal of Adıyaman University 2019 Vol.5 Issue 2, pp.1500-1510

Belge Tipi: Makale

Yayın Tarihi: 8/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000441109

Sıklık: Üç Yıllık

eISSN: 2458-9179

DOI: https://doi.org/10.30569/adiyamansaglik.596944

Sayı

Yazarlar:

Saliha Ayşenur ÇAM | Seyfullah Oktay ARSLAN

Yayıncı:

Adıyaman University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Health Sciences Journal of Adıyaman University 2019 Vol.5 Issue 2, pp.1500-1510

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000441109

Sıklık:

Üç Yıllık

eISSN:

2458-9179

DOI:

https://doi.org/10.30569/adiyamansaglik.596944