Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

Rahul NARASANNA

doi:https://doi.org/10.5799/jmid.328786

Makale detayları

Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

Yazarlar:

Rahul NARASANNA

Yayıncı:

Sağlık Araştırmaları Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 02, pp.67-74

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000487483

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.328786

Özet:

 

Objectives: In the present study, we have analyzed ESBL-producing S. typhi’s capability in forming a significant
amount of biofilm on plastic and glass surface, and the influence of cefetoxime on biofilm development at
subinhibitory (Sub-MIC) concentration.
Methods: Nine strains of cefetoxime-mediated ESBL-producing S. typhi were used in the study. S. typhi formed
biofilm on plastic and glass materials; it was demonstrated using micro titre plate (MTP) and standard test tube
methods. Comparative study of the influence of cefetoxime on biofilm formation in its MIC (128 µg/ml) and at sub-MIC
(64 µg/ml) was demonstrated by microtitre plate method. The biofilm production was observed in SEM images,
statistical analysis (ANOVA) showed significant increase in cell surface and volume due to the influence of
Cefetoxime.
Results: Of the nine selected isolates, two S. typhi strains, namely BST 51 and BST 130, produced relatively strong
biofilm in the presence of cefetoxime at sub-MIC level (64 µg/ml), comparatively weak biofilm formation at MIC level
(128 µg/ml). Typical morphological changes were observed in cefetoxime-resistant strains, S. typhi BST 51 and BST
130, in comparison to cefetoxime-sensitive strain S. typhi BST 63 used as a control. We found an increase in surface
and volume of a cell in response to cefetoxime and statistical data (ANOVA) proved that resistant strains were
significantly different from control strains.
Conclusion: The above study clearly shows that cefetoxime at sub-MIC level efficiently induces biofilm formation
and promotes changes in morphology of the cell. J Microbiol Infect Dis 2017; 7(2): 67-75 .

Makale detayları

Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

Yazarlar: Rahul NARASANNA

Yayıncı: Sağlık Araştırmaları Derneği

Ana Başlıklar: Sağlık teşvikleri

Dergi: Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 02, pp.67-74

Belge Tipi: Makale

Yayın Tarihi: 5/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000487483

Sıklık: Üç Aylık

eISSN: 2146-9369

pISSN: 2146-3158

DOI: https://doi.org/10.5799/jmid.328786

Sayı

Yazarlar:

Rahul NARASANNA

Yayıncı:

Sağlık Araştırmaları Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 02, pp.67-74

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000487483

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.328786