Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells

Natalia GRIGOROVA; Elen GÓCZA; Ekaterina VACHKOVA

doi:https://doi.org/10.33988/auvfd.682682

Makale detayları

Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells

Yazarlar:

Natalia GRIGOROVA | Elen GÓCZA | Ekaterina VACHKOVA

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 4, pp.407-412

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849448

DOI:

https://doi.org/10.33988/auvfd.682682

Özet:

 

Cardiovascular diseases are still one of the most common reasons for mortality in humans. Mesenchymal stem cells (MSCs) are preferable in cardiac regeneration cell-based therapies because of their allogeneic and high proliferative potential. The electrophysiological properties of the rabbit heard is closer to human than the mouse. The current study aimed to trace mRNA expression changes of two stemness/cardiogenic differentiation ability-related transcriptionala factors OCT4 and GATA4 in rabbit MSCs during early stages of induced cardiomyocyte differentiation in vitro. The mesenchymal stem cell originated from different anatomical areas-subcutaneous, visceral, bone marrow and pericardial tissue. The cardiac differentiation protocol for mouse embryonic stem cells in hanging drop was adopted in rabbit MSCs. The best formed embryonal bodies (EBs) like structures were collected and cultivated on gelatin-coated plates. The total mRNA was obtained before cardiac differentiation and on the 6th day after it. SYBER based real-time PCR was performed to evaluate the mRNA expression fold-changes of OCT4 and GATA4. The cultivation of MSCs in hanging drops during cardiac differentiation induced EBs formation, without any contractile activity up to the 6th day of the differentiation in all cell types. The applied differentiation protocol significantly downregulated GATA4 expression in ADSCs - EBs, while in BMSCs, both target genes were significantly upregulated. In conclusion, the adopted cardiac differentiation protocol from mouse embryonic stem cells could be a useful approach for rabbit bone marrow mesenchymal stem cells. Since the rest of the cells revealed weak cardiogenic capability at this early stage, some modifications of induction protocols should be considered.

Makale detayları

Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells

Yazarlar: Natalia GRIGOROVA | Elen GÓCZA | Ekaterina VACHKOVA

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 4, pp.407-412

Belge Tipi: Makale

Yayın Tarihi: 8/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000849448

DOI: https://doi.org/10.33988/auvfd.682682

Sayı

Yazarlar:

Natalia GRIGOROVA | Elen GÓCZA | Ekaterina VACHKOVA

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 4, pp.407-412

Belge Tipi:

Makale

Yayın Tarihi:

8/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849448

DOI:

https://doi.org/10.33988/auvfd.682682