THE PREFERENCE PRIORITY OF Bacillus subtilis IN UPTAKING FREE DNA DURING THE NATURAL TRANSFORMATION

Musa Tartık

Makale detayları

THE PREFERENCE PRIORITY OF Bacillus subtilis IN UPTAKING FREE DNA DURING THE NATURAL TRANSFORMATION

Yazarlar:

Musa Tartık

Yayıncı:

Trakya University

Ana Başlıklar:

Genetics

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287850

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 Although genetic material is vertically transferred between generations via sexual or asexual reproduction, similarities in some chromosome and gene parts of unrelated organisms provide important clues for another way of transfer. The mobility of genetic information among different organisms, known as horizontal gene transfer HGT has immediate or delayed effects on the recipient host. One of the most notable mechanisms of HGT is natural transformation NT, a process in which cells take free DNA from the extracellular environment and incorporate it into their chromosomes by homologous recombination. NT is widely conserved in many bacterial species as it can promote to spread of resistance genes. Although it is known that many organisms rely on HGT, there is limited information about how they decide which particular genetic material to horizontally transfer. Here, I have investigated the preference priority among different gene sources presented under certain stress conditions for Bacillus subtilis possessing NT ability. To test this, two DNA specimens E and C with different sequence contents of the same length were presented to B. subtilis under different stress environments BK, BC, BE and BCE. The hypothesis was evaluated according to the analysis of the results of colonial formations on selective plates pE, pC and pCE. The obtained data presented a strong positive correlation that the bacteria have preference priority during NT depending on a stimulator. The tendency of the bacteria to uptake useful DNA fragments in a specific environment can be suggested. For instance, the majority of colonies grow on pE plates rather than the pC and pCE when the transformation media includes erythromycin Eryt as an inducer. Although the data significantly overlaps with the idea claiming that the bacteria have a preference priority to uptake free DNAs during NT, further investigations are needed to support the present data and for better understanding of the phenomenon.

 Although genetic material is vertically transferred between generations via sexual or asexual reproduction, similarities in some chromosome and gene parts of unrelated organisms provide important clues for another way of transfer. The mobility of genetic information among different organisms, known as horizontal gene transfer HGT has immediate or delayed effects on the recipient host. One of the most notable mechanisms of HGT is natural transformation NT, a process in which cells take free DNA from the extracellular environment and incorporate it into their chromosomes by homologous recombination. NT is widely conserved in many bacterial species as it can promote to spread of resistance genes. Although it is known that many organisms rely on HGT, there is limited information about how they decide which particular genetic material to horizontally transfer. Here, I have investigated the preference priority among different gene sources presented under certain stress conditions for Bacillus subtilis possessing NT ability. To test this, two DNA specimens E and C with different sequence contents of the same length were presented to B. subtilis under different stress environments BK, BC, BE and BCE. The hypothesis was evaluated according to the analysis of the results of colonial formations on selective plates pE, pC and pCE. The obtained data presented a strong positive correlation that the bacteria have preference priority during NT depending on a stimulator. The tendency of the bacteria to uptake useful DNA fragments in a specific environment can be suggested. For instance, the majority of colonies grow on pE plates rather than the pC and pCE when the transformation media includes erythromycin Eryt as an inducer. Although the data significantly overlaps with the idea claiming that the bacteria have a preference priority to uptake free DNAs during NT, further investigations are needed to support the present data and for better understanding of the phenomenon.

 Although genetic material is vertically transferred between generations via sexual or asexual reproduction, similarities in some chromosome and gene parts of unrelated organisms provide important clues for another way of transfer. The mobility of genetic information among different organisms, known as horizontal gene transfer HGT has immediate or delayed effects on the recipient host. One of the most notable mechanisms of HGT is natural transformation NT, a process in which cells take free DNA from the extracellular environment and incorporate it into their chromosomes by homologous recombination. NT is widely conserved in many bacterial species as it can promote to spread of resistance genes. Although it is known that many organisms rely on HGT, there is limited information about how they decide which particular genetic material to horizontally transfer. Here, I have investigated the preference priority among different gene sources presented under certain stress conditions for Bacillus subtilis possessing NT ability. To test this, two DNA specimens E and C with different sequence contents of the same length were presented to B. subtilis under different stress environments BK, BC, BE and BCE. The hypothesis was evaluated according to the analysis of the results of colonial formations on selective plates pE, pC and pCE. The obtained data presented a strong positive correlation that the bacteria have preference priority during NT depending on a stimulator. The tendency of the bacteria to uptake useful DNA fragments in a specific environment can be suggested. For instance, the majority of colonies grow on pE plates rather than the pC and pCE when the transformation media includes erythromycin Eryt as an inducer. Although the data significantly overlaps with the idea claiming that the bacteria have a preference priority to uptake free DNAs during NT, further investigations are needed to support the present data and for better understanding of the phenomenon.

 Genetik materyal nesiller arasında genellikle eşeyli veya eşeysiz üreme yoluyla dikey olarak aktarılsa da, akraba olmayan organizmaların bazı kromozom ve gen kısımlarındaki büyük benzerlikler, başka bir aktarım yolu olabileceğini gösterir. Farklı organizmalar arasında genetik bilginin hareketliliği olarak bilinen yatay gen transferi YGT, alıcı konak üzerinde ani veya gecikmeli etkilere sahiptir. YGTnin en dikkate değer mekanizmalarından biri, hücrelerin hücre dışı ortamdan serbest DNA aldığı ve homolog rekombinasyon yoluyla kromozomlarına dâhil ettiği bir süreç olan doğal transformasyondur DT. DT, direnç genlerinin yayılmasını teşvik edebildiği için birçok bakteri türünde yaygın olarak korunur. Birçok organizmanın YGT gerçekleştirdiği bilinmesine rağmen, organizmaların yatay olarak aktarılan genetik materyale nasıl karar verdiği hakkındaki bilgi sınırlıdır. Burada, DT yeteneğine sahip Bacillus subtilisin belirli stres koşulları altında sunulan farklı gen kaynakları arasından seçim önceliğini araştırdım. Bunu test etmek için, aynı uzunlukta fakat farklı dizi içeriğine sahip iki DNA örneği E ve C, farklı stres ortamları BK, BC, BE ve BCE altında B. subtilise sunuldu. Hipotez, DT sonrasında seçici plakalar pE, pC ve pCE üzerinde oluşan kolonilerin analiz sonuçlarına göre değerlendirildi. Elde edilen veriler, DT sırasında bakterilerin bir uyarıcıya bağlı olarak tercih önceliğine sahip olduğuna dair güçlü bir pozitif korelasyon sunmuştur. Bakterilerin belirli bir ortamda yararlı DNA parçalarını alma eğilimi gösterdiği söylenebilir, örneğin, dönüştürme ortamı bir indükleyici olarak erythromycin Eryt içerdiğinde kolonilerin çoğunluğu pC ve pCE yerine pE plakaları üzerinde büyümüştür. Veriler, bakterilerin DT sırasında serbest DNAları almak için bir tercih önceliğine sahip olduğu iddiasıyla önemli ölçüde örtüşse de, verileri güçlü bir şekilde desteklemek ve fenomeni doğru bir şekilde anlamak için daha fazla araştırmaya ihtiyaç vardır.

Makale detayları

THE PREFERENCE PRIORITY OF Bacillus subtilis IN UPTAKING FREE DNA DURING THE NATURAL TRANSFORMATION

Yazarlar:

Musa Tartık

Yayıncı:

Trakya University

Ana Başlıklar:

Genetics

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287850

pISSN:

2528-9691

Horizontal gene transfer

Natural competence

Bacteria

DNA uptake mechanism

Sayı

Makale detayları

THE PREFERENCE PRIORITY OF Bacillus subtilis IN UPTAKING FREE DNA DURING THE NATURAL TRANSFORMATION

Yazarlar: Musa Tartık

Yayıncı: Trakya University

Ana Başlıklar: Genetics

Dergi: Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.31-39

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287850

pISSN: 2528-9691

Horizontal gene transfer

Natural competence

Bacteria

DNA uptake mechanism

Sayı

Yazarlar:

Musa Tartık

Yayıncı:

Trakya University

Ana Başlıklar:

Genetics

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.31-39

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287850

pISSN:

2528-9691