THE EFFECT OF α-MELANOCYTE STIMULATING HORMONE ON BURN-INDUCED OXIDANT PRODUCTION BY RAT PERITONEAL NEUTROPHILS

Berna OKTAR; Meral YÜKSEL; İnci ALİCAN

Makale detayları

THE EFFECT OF α-MELANOCYTE STIMULATING HORMONE ON BURN-INDUCED OXIDANT PRODUCTION BY RAT PERITONEAL NEUTROPHILS

Yazarlar:

Berna OKTAR | Meral YÜKSEL | İnci ALİCAN

Yayıncı:

Marmara University

Ana Başlıklar:

Hastalıklar

Dergi:

Marmara Medical Journal 2003 Vol.16 Issue 1, pp.7-11

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000546252

Sıklık:

Aylık

eISSN:

1309-9469

pISSN:

1019-1941

Özet:

 

Objective: Previous findings related to the anti-inflammatory effects of α-melanocyte stimulating hormone (α-MSH) indicate that the peptide might inhibit inflammation by antagonizing the effects of local cytokines or by inhibiting neutrophil functions. In addition, it is a potent inhibitor of the induction of the inducible nitric oxide synthase (iNOS) in cultured macrophages and of nitric oxide (NO) production in a sepsis model. This study was designed to investigate whether α-MSH affects reactive oxygen metabolite production by rat peritoneal neutrophils following burn injury and whether NO is involved in this effect.

Methods: The neutrophils were removed from the peritoneum of the rats 6 h after burn or sham trauma. Cells (2 X 106 /ml) were treated with the increasing concentrations of α-MSH (10–12 M, 10–10 M, 10–8 M or 10–6 M). Nitric oxide donor, sodium nitroprusside ranging from (10–10 - 10–4 M) was added to the cells in the presence of α-MSH. Reactive oxygen metabolite production was measured by chemiluminescence (CL) technique using luminol or lucigenin probes.

Results: Lucigenin CL value of cells obtained from burn animals was significantly higher than those obtained from the sham group. α-MSH caused significant reductions in high lucigenin CL values and this effect was partly inhibited by sodium nitroprusside.

Conclusion: Our results suggest that α-MSH is effective in preventing oxidant production by neutrophils activated by burn trauma, at least in part, by a mechanism involving nitric oxide. 

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Makale detayları

THE EFFECT OF α-MELANOCYTE STIMULATING HORMONE ON BURN-INDUCED OXIDANT PRODUCTION BY RAT PERITONEAL NEUTROPHILS

Yazarlar: Berna OKTAR | Meral YÜKSEL | İnci ALİCAN

Yayıncı: Marmara University

Ana Başlıklar: Hastalıklar

Dergi: Marmara Medical Journal 2003 Vol.16 Issue 1, pp.7-11

Belge Tipi: Makale

Yayın Tarihi: 12/2016

Belge Dili: İngilizce

Document ID (Org No.): 2000546252

Sıklık: Aylık

eISSN: 1309-9469

pISSN: 1019-1941

Sayı

Yazarlar:

Berna OKTAR | Meral YÜKSEL | İnci ALİCAN

Yayıncı:

Marmara University

Ana Başlıklar:

Hastalıklar

Dergi:

Marmara Medical Journal 2003 Vol.16 Issue 1, pp.7-11

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000546252

Sıklık:

Aylık

eISSN:

1309-9469

pISSN:

1019-1941