mTOR signaling pathway genes effect in COVID-19 infection

Mukaddes Pala; Şenay Görücü Yılmaz; Elif Seren Tanriverdi; Leman Acun Delen; Dilara Altay Ozturk; Mesut Öterkuş

Makale detayları

mTOR signaling pathway genes effect in COVID-19 infection

Yazarlar:

Mukaddes Pala | Şenay Görücü Yılmaz | Elif Seren Tanriverdi | Leman Acun Delen | Dilara Altay Ozturk | Mesut Öterkuş

Yayıncı:

Inonu University

Ana Başlıklar:

Gene Expression

Dergi:

Anatolian Journal of Pharmaceutical Sciences Vol.3 Issue 2, pp.191-206

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328705

pISSN:

2980-0463

Anahtar kelimeler:

Özet:

 Coronavirus disease 2019 Covid-19 is an infectious disease that causes severe acute respiratory illness caused by coronavirus 2 SARS-CoV-2. SARS-CoV-2 uses host-specific metabolic pathways, including mTOR. The mTOR pathway is hyperactive in viral respiratory tract infections and contributes positively to viral replication. 100 samples were evaluated, 50 patients Female23, Male27, and 50 controls Female29, Male21. The patients were individuals who were COVID-19 positive. We detected expression changes of 5 genes in mTOR complex 1 mTORC1 and mTOR complex 2 mTORC2 MLST8, mTOR, RPTOR, MAPKAP1 and RICTOR. Serum samples were obtained from all patients. The expression changes of mTORC1 and mTORC2 Complex genes were evaluated with Real-time PCR method. Receiver operating curve ROC analysis was performed to define the diagnostic power of these genes. Expression changes of five genes in the mTORC1 and mTORC2 complex were statistically significant p 0.001 and upregulated in serum. The area under the ROC Curve values indicating the diagnostic power of genes were 0.948, 0.771, 0.851, 0.798, and 0.805, respectively. These genes may be candidate for treatment targets. The high discriminative power of these genes in patients from controls indicates their diagnostic potential in serum samples.

 Coronavirus disease 2019 Covid-19 is an infectious disease that causes severe acute respiratory illness caused by coronavirus 2 SARS-CoV-2. SARS-CoV-2 uses host-specific metabolic pathways, including mTOR. The mTOR pathway is hyperactive in viral respiratory tract infections and contributes positively to viral replication. 100 samples were evaluated, 50 patients Female23, Male27, and 50 controls Female29, Male21. The patients were individuals who were COVID-19 positive. We detected expression changes of 5 genes in mTOR complex 1 mTORC1 and mTOR complex 2 mTORC2 MLST8, mTOR, RPTOR, MAPKAP1 and RICTOR. Serum samples were obtained from all patients. The expression changes of mTORC1 and mTORC2 Complex genes were evaluated with Real-time PCR method. Receiver operating curve ROC analysis was performed to define the diagnostic power of these genes. Expression changes of five genes in the mTORC1 and mTORC2 complex were statistically significant p 0.001 and upregulated in serum. The area under the ROC Curve values indicating the diagnostic power of genes were 0.948, 0.771, 0.851, 0.798, and 0.805, respectively. These genes may be candidate for treatment targets. The high discriminative power of these genes in patients from controls indicates their diagnostic potential in serum samples.

 Coronavirus disease 2019 Covid-19 is an infectious disease that causes severe acute respiratory illness caused by coronavirus 2 SARS-CoV-2. SARS-CoV-2 uses host-specific metabolic pathways, including mTOR. The mTOR pathway is hyperactive in viral respiratory tract infections and contributes positively to viral replication. 100 samples were evaluated, 50 patients Female23, Male27, and 50 controls Female29, Male21. The patients were individuals who were COVID-19 positive. We detected expression changes of 5 genes in mTOR complex 1 mTORC1 and mTOR complex 2 mTORC2 MLST8, mTOR, RPTOR, MAPKAP1 and RICTOR. Serum samples were obtained from all patients. The expression changes of mTORC1 and mTORC2 Complex genes were evaluated with Real-time PCR method. Receiver operating curve ROC analysis was performed to define the diagnostic power of these genes. Expression changes of five genes in the mTORC1 and mTORC2 complex were statistically significant p 0.001 and upregulated in serum. The area under the ROC Curve values indicating the diagnostic power of genes were 0.948, 0.771, 0.851, 0.798, and 0.805, respectively. These genes may be candidate for treatment targets. The high discriminative power of these genes in patients from controls indicates their diagnostic potential in serum samples.

 Coronavirus disease 2019 Covid-19 is an infectious disease that causes severe acute respiratory illness caused by coronavirus 2 SARS-CoV-2. SARS-CoV-2 uses host-specific metabolic pathways, including mTOR. The mTOR pathway is hyperactive in viral respiratory tract infections and contributes positively to viral replication. 100 samples were evaluated, 50 patients Female23, Male27, and 50 controls Female29, Male21. The patients were individuals who were COVID-19 positive. We detected expression changes of 5 genes in mTOR complex 1 mTORC1 and mTOR complex 2 mTORC2 MLST8, mTOR, RPTOR, MAPKAP1 and RICTOR. Serum samples were obtained from all patients. The expression changes of mTORC1 and mTORC2 Complex genes were evaluated with Real-time PCR method. Receiver operating curve ROC analysis was performed to define the diagnostic power of these genes. Expression changes of five genes in the mTORC1 and mTORC2 complex were statistically significant p 0.001 and upregulated in serum. The area under the ROC Curve values indicating the diagnostic power of genes were 0.948, 0.771, 0.851, 0.798, and 0.805, respectively. These genes may be candidate for treatment targets. The high discriminative power of these genes in patients from controls indicates their diagnostic potential in serum samples.

Makale detayları

mTOR signaling pathway genes effect in COVID-19 infection

Yazarlar:

Mukaddes Pala | Şenay Görücü Yılmaz | Elif Seren Tanriverdi | Leman Acun Delen | Dilara Altay Ozturk | Mesut Öterkuş

Yayıncı:

Inonu University

Ana Başlıklar:

Gene Expression

Dergi:

Anatolian Journal of Pharmaceutical Sciences Vol.3 Issue 2, pp.191-206

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328705

pISSN:

2980-0463

COVID-19

SARS-CoV-2

mTOR complex 1 (mTORC1)

mTOR complex 2 (mTORC2)

Sayı

Makale detayları

mTOR signaling pathway genes effect in COVID-19 infection

Yazarlar: Mukaddes Pala | Şenay Görücü Yılmaz | Elif Seren Tanriverdi | Leman Acun Delen | Dilara Altay Ozturk | Mesut Öterkuş

Yayıncı: Inonu University

Ana Başlıklar: Gene Expression

Dergi: Anatolian Journal of Pharmaceutical Sciences Vol.3 Issue 2, pp.191-206

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328705

pISSN: 2980-0463

COVID-19

SARS-CoV-2

mTOR complex 1 (mTORC1)

mTOR complex 2 (mTORC2)

Sayı

Yazarlar:

Mukaddes Pala | Şenay Görücü Yılmaz | Elif Seren Tanriverdi | Leman Acun Delen | Dilara Altay Ozturk | Mesut Öterkuş

Yayıncı:

Inonu University

Ana Başlıklar:

Gene Expression

Dergi:

Anatolian Journal of Pharmaceutical Sciences Vol.3 Issue 2, pp.191-206

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328705

pISSN:

2980-0463