OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

Aysan LEKTEMÜR ALPAN; Hülya TOKER; Hatice ÖZER

Makale detayları

OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

Yazarlar:

Aysan LEKTEMÜR ALPAN | Hülya TOKER | Hatice ÖZER

Yayıncı:

Selcuk University

Ana Başlıklar:

Cerrahi ve ilgili tıbbi uzmanlıklar

Dergi:

Selcuk Dental Journal 2020 Vol.7 Issue 1, pp.44-53

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000575442

Sıklık:

Aylık

eISSN:

2148-7529

Özet:

 


Backround: Diabetes
mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone
have several actions such as
antimicrobial ,immunostimulating, antihypoxic effects, and activates
antioxidant enzymes and angiogenesis


Aim:
The aim of this study is to investigate the
effect of gaseous ozone on bone healing in diabetic rat calvarial defects
treated with autogenous bone graft, morphometrically and immunohistochemically.


Material and Methods:
Diabetes was induced and critical size defects were created on rats. Study
groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group,
3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone
application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation
day and the following 2 weeks daily (140ppm @
2L/d, 2.24 mg). Total bone area was measured. Osteocalcin and Bone morphogenic
protein-2  protein expressions were evaluated.















KEYWORDS: autograft, bone regeneration, calvarial defect, experimental
diabetes mellitus, ozone




.

 


Amaç: Diabetes
mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone
have several actions such as
antimicrobial ,immunostimulating, antihypoxic effects, and activates
antioxidant enzymes and angiogenesis. The aim of this study is to investigate the effect of gaseous ozone on
bone healing in diabetic rat calvarial defects treated with autogenous bone
graft, morphometrically and immunohistochemically



Gereç ve Yöntemler:Diabetes
was induced and critical size defects were created on rats. Study groups:
1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty
defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application
(AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the
following 2 weeks daily (140ppm @ 2L/d, 2.24 mg).
Total bone area was measured.
Osteocalcin and
Bone morphogenic
protein-2  protein expressions were evaluated.



Bulgular:Control and Control+Ozone groups had no
osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone
group were higher than AG group at 4th week (p>0.05). AG+Ozone group had
more total bone area than AG group at 4th week. AG+Ozone group revealed more
BMP-2 immune positivity compared to the other groups. Osteocalcin immune
positivity in AG groups was higher than those of the Control groups.

Sonuçlar:

Anahtar Kelimeler:

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Makale detayları

OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

Yazarlar: Aysan LEKTEMÜR ALPAN | Hülya TOKER | Hatice ÖZER

Yayıncı: Selcuk University

Ana Başlıklar: Cerrahi ve ilgili tıbbi uzmanlıklar

Dergi: Selcuk Dental Journal 2020 Vol.7 Issue 1, pp.44-53

Belge Tipi: Makale

Yayın Tarihi: 3/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000575442

Sıklık: Aylık

eISSN: 2148-7529

Sayı

Yazarlar:

Aysan LEKTEMÜR ALPAN | Hülya TOKER | Hatice ÖZER

Yayıncı:

Selcuk University

Ana Başlıklar:

Cerrahi ve ilgili tıbbi uzmanlıklar

Dergi:

Selcuk Dental Journal 2020 Vol.7 Issue 1, pp.44-53

Belge Tipi:

Makale

Yayın Tarihi:

3/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000575442

Sıklık:

Aylık

eISSN:

2148-7529