The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

Ebru Şirin; Bahadır Altun; Ahmet Kazankaya

Makale detayları

The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

Yazarlar:

Ebru Şirin | Bahadır Altun | Ahmet Kazankaya

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.631-635

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303242

pISSN:

2618-6578

Anahtar kelimeler:

Özet:

 In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg L IBA  1.0 mg L BAP and 1.0 mgL BAP  1.0 mgL NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract 20K obtained from oven-dried walnut shell can be used instead of chemical sterilant.

 In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg L IBA  1.0 mg L BAP and 1.0 mgL BAP  1.0 mgL NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract 20K obtained from oven-dried walnut shell can be used instead of chemical sterilant.

 In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg L IBA  1.0 mg L BAP and 1.0 mgL BAP  1.0 mgL NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract 20K obtained from oven-dried walnut shell can be used instead of chemical sterilant.

 In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg L IBA  1.0 mg L BAP and 1.0 mgL BAP  1.0 mgL NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract 20K obtained from oven-dried walnut shell can be used instead of chemical sterilant.

Makale detayları

The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

Yazarlar:

Ebru Şirin | Bahadır Altun | Ahmet Kazankaya

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.631-635

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303242

pISSN:

2618-6578

Rosa canina L.

Walnut shell extract

Tissue culture

Antimicrobial activity

Antifungal activity

Sayı

Makale detayları

The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

Yazarlar: Ebru Şirin | Bahadır Altun | Ahmet Kazankaya

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Black Sea Journal of Agriculture Vol.6 Issue 6, pp.631-635

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001303242

pISSN: 2618-6578

Rosa canina L.

Walnut shell extract

Tissue culture

Antimicrobial activity

Antifungal activity

Sayı

Yazarlar:

Ebru Şirin | Bahadır Altun | Ahmet Kazankaya

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Black Sea Journal of Agriculture Vol.6 Issue 6, pp.631-635

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001303242

pISSN:

2618-6578