Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province,  Turkey

Abdullah Güller; Mustafa Usta; Zeynelabidin Kurt

Makale detayları

Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey

Yazarlar:

Abdullah Güller | Mustafa Usta | Zeynelabidin Kurt

Yayıncı:

Igdir University

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.44-53

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284038

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Viruses infecting honey bees are diseases that cause infection and economic damage in every developmental stage of honey bees all over the world. In this study, a survey was conducted to determine the presence and prevalence of two worldwide common honeybee viruses deformed wing virus DWV and chronic bee paralysis virus CBPV. 128 apiaries from 9 different localities in the province of Bingöl were visited to determine viral RNA. Collected 384 honey bee samples were tested molecularly reverse-transcriptase polymerase chain reaction, RTPCR using genome-specific primers specific to each virus. In molecular tests, 28 of 128 apiaries 21.87 gave a positive reaction for DWV, but no CBPV pathogen was detected in any apiary. The 711 bp nucleotide sequence revealed by cloning one of the randomly selected positive samples was registered in the gene bank with the accession number MZ357973. According to the BLASTn analysis in the NCBI database, the nucleotide sequence of the DWV agent determined in Bingöl Province showed nucleotide similarity between 77.82-98.45 when compared to other isolates of the same virus. In addition, our isolate was found to be in a close phylogenetic relationship with the Erzincan-DWV isolate from Turkey, based on the phylogenetic tree using isolates from different origins. According to our literature screening, this study is the first record that reveals the prevalence and the presence of DWV and CBPV in Bingöl Province with DNA-based approaches.

 Viruses infecting honey bees are diseases that cause infection and economic damage in every developmental stage of honey bees all over the world. In this study, a survey was conducted to determine the presence and prevalence of two worldwide common honeybee viruses deformed wing virus DWV and chronic bee paralysis virus CBPV. 128 apiaries from 9 different localities in the province of Bingöl were visited to determine viral RNA. Collected 384 honey bee samples were tested molecularly reverse-transcriptase polymerase chain reaction, RTPCR using genome-specific primers specific to each virus. In molecular tests, 28 of 128 apiaries 21.87 gave a positive reaction for DWV, but no CBPV pathogen was detected in any apiary. The 711 bp nucleotide sequence revealed by cloning one of the randomly selected positive samples was registered in the gene bank with the accession number MZ357973. According to the BLASTn analysis in the NCBI database, the nucleotide sequence of the DWV agent determined in Bingöl Province showed nucleotide similarity between 77.82-98.45 when compared to other isolates of the same virus. In addition, our isolate was found to be in a close phylogenetic relationship with the Erzincan-DWV isolate from Turkey, based on the phylogenetic tree using isolates from different origins. According to our literature screening, this study is the first record that reveals the prevalence and the presence of DWV and CBPV in Bingöl Province with DNA-based approaches.

 Viruses infecting honey bees are diseases that cause infection and economic damage in every developmental stage of honey bees all over the world. In this study, a survey was conducted to determine the presence and prevalence of two worldwide common honeybee viruses deformed wing virus DWV and chronic bee paralysis virus CBPV. 128 apiaries from 9 different localities in the province of Bingöl were visited to determine viral RNA. Collected 384 honey bee samples were tested molecularly reverse-transcriptase polymerase chain reaction, RTPCR using genome-specific primers specific to each virus. In molecular tests, 28 of 128 apiaries 21.87 gave a positive reaction for DWV, but no CBPV pathogen was detected in any apiary. The 711 bp nucleotide sequence revealed by cloning one of the randomly selected positive samples was registered in the gene bank with the accession number MZ357973. According to the BLASTn analysis in the NCBI database, the nucleotide sequence of the DWV agent determined in Bingöl Province showed nucleotide similarity between 77.82-98.45 when compared to other isolates of the same virus. In addition, our isolate was found to be in a close phylogenetic relationship with the Erzincan-DWV isolate from Turkey, based on the phylogenetic tree using isolates from different origins. According to our literature screening, this study is the first record that reveals the prevalence and the presence of DWV and CBPV in Bingöl Province with DNA-based approaches.

 Bal arılarını infekteleyen virüsler dünyanın her yerinde bal arılarının her gelişim evresinde infeksiyona neden olan ve ekonomik zarara yol açan hastalıklardır. Bu araştırmada dünya çapında yaygın olan iki bal arısı virüsünün deforme kanat virüsü DWV ve kronik arı paraliz virüsü CBPV varlığı ve yaygınlığını belirlemek için bir survey çalışması gerçekleştirilmiştir. Viral RNAyı belirlemek amacıyla Bingöl ili sınırlarındaki 9 farklı lokaliteden toplam 128 arılık ziyaret edilmiştir. Toplanan 384 bal arısı örneği her virüse özgü genom spesifik primerler kullanılarak moleküler olarak revers-transkriptaz polimeraz zincir reaksiyonu, RT-PCR testlenmiştir. 128 arılığın 28i 21.87 DWV için pozitif reaksiyon vermiştir fakat hiçbir arılıkta CBPV patojeni tespit edilmemiştir. Pozitif örneklerden rastgele seçilen birinin klonlanarak ortaya çıkarılan 702 bazlık nükleotit dizisi gen bankasına MZ357973 ulaşım numarasıyla kaydedilmiştir. NCBI veri tabanındaki BLASTn analizine göre, Bingöl ilinde belirlenen DWV etmeninin nükleotit dizisi aynı virüsün diğer izolatlarıyla kıyaslandığında 77.82-98.45 arasında değişen nükleotit benzerliği göstermiştir. Ayrıca, farklı orijinlerden izolatların kullanıldığı filogenetik ağaca dayanarak bizim izolatımız Türkiyeden ErzincanDWV izolatıyla yakın filogenetik ilişki içerisinde olduğu tespit edilmiştir. Literatür taramalarına göre, bu çalışma Bingöl ilinde DWV ve CBPV yaygınlığını ve varlığını DNA temelli yöntemlerle ortaya koyan ilk kayıt niteliğindedir.

Makale detayları

Prevalence of Deformed wing virus and Chronic bee paralysis virus in Honey Bee Colonies of Bingöl province, Turkey

Yazarlar:

Abdullah Güller | Mustafa Usta | Zeynelabidin Kurt

Yayıncı:

Igdir University

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.44-53

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284038

pISSN:

2536-4618

DWV

CBPV

Turkey

Apis mellifera

Prevelence

DWV

CBPV

Yayılım

Türkiye

Apis mellifera

Sayı

Makale detayları

Yazarlar: Abdullah Güller | Mustafa Usta | Zeynelabidin Kurt

Yayıncı: Igdir University

Ana Başlıklar: Hayvansal Üretim (Diğer)

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.44-53

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001284038

pISSN: 2536-4618

Sayı

Yazarlar:

Abdullah Güller | Mustafa Usta | Zeynelabidin Kurt

Yayıncı:

Igdir University

Ana Başlıklar:

Hayvansal Üretim (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.44-53

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284038

pISSN:

2536-4618