Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activitiess

Nilgün Güler; Halime Güzin Aslan; Ercan Karaköse; Mithat Güllü

Makale detayları

Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activitiess

Yazarlar:

Nilgün Güler | Halime Güzin Aslan | Ercan Karaköse | Mithat Güllü

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Enzymes | Molecular Ecology | Molecular Medicine

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.166-180

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357696

pISSN:

1304-7981

Anahtar kelimeler:

Özet:

 This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide ZnO nanoparticles NPs synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica apple and Tilia cordata linden plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction XRD, Scanning Electron Microscopy SEM, and Fourier Transform Infrared FTIR analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs biomass were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mgmL concentration of CT-DNA.

 This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide ZnO nanoparticles NPs synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica apple and Tilia cordata linden plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction XRD, Scanning Electron Microscopy SEM, and Fourier Transform Infrared FTIR analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs biomass were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mgmL concentration of CT-DNA.

 This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide ZnO nanoparticles NPs synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica apple and Tilia cordata linden plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction XRD, Scanning Electron Microscopy SEM, and Fourier Transform Infrared FTIR analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs biomass were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mgmL concentration of CT-DNA.

 This study aimed to evaluate the anticancer and antibacterial drug status of biomass, including zinc oxide ZnO nanoparticles NPs synthesized from two plant extracts according to the results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial activity tests. The Malus domestica apple and Tilia cordata linden plants were dried and powdered, and their extracts were prepared in pure water. X-Ray Diffraction XRD, Scanning Electron Microscopy SEM, and Fourier Transform Infrared FTIR analyses were applied for the characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis method. Powder ZnO NPs biomass were analyzed by the agar well diffusion method by using two gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In ZnO NPs from linden extract, there was an interaction at a 1mgmL concentration of CT-DNA.

Makale detayları

Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activitiess

Yazarlar:

Nilgün Güler | Halime Güzin Aslan | Ercan Karaköse | Mithat Güllü

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Enzymes | Molecular Ecology | Molecular Medicine

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.166-180

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357696

pISSN:

1304-7981

Enzyme inhibition

green synthesis

inorganic drug

anti-bacterial activity

ZnO NPs

Sayı

Makale detayları

Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activitiess

Yazarlar: Nilgün Güler | Halime Güzin Aslan | Ercan Karaköse | Mithat Güllü

Yayıncı: Tokat Gaziosmanpasa University

Ana Başlıklar: Enzymes | Molecular Ecology | Molecular Medicine

Dergi: Journal of New Results in Science Vol.14 Issue 2, pp.166-180

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357696

pISSN: 1304-7981

Enzyme inhibition

green synthesis

inorganic drug

anti-bacterial activity

ZnO NPs

Sayı

Yazarlar:

Nilgün Güler | Halime Güzin Aslan | Ercan Karaköse | Mithat Güllü

Yayıncı:

Tokat Gaziosmanpasa University

Ana Başlıklar:

Enzymes | Molecular Ecology | Molecular Medicine

Dergi:

Journal of New Results in Science Vol.14 Issue 2, pp.166-180

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357696

pISSN:

1304-7981