INVESTIGATION OF THE PROTECTIVE EFFECT OF OMEGA-3 FATTY ACIDS ON MONOSODIUM GLUTAMATE-INDUCED HIPPOCAMPAL INJURY

Hayrunnisa Yesil Sarsmaz; Seren Gülşen Gürgen

Makale detayları

INVESTIGATION OF THE PROTECTIVE EFFECT OF OMEGA-3 FATTY ACIDS ON MONOSODIUM GLUTAMATE-INDUCED HIPPOCAMPAL INJURY

Yazarlar:

Hayrunnisa Yesil Sarsmaz | Seren Gülşen Gürgen

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.21-29

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313403

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective Monosodium glutamate MSG is a flavor enhancer that is added to many ready-to-eat foods and is known to be excitotoxic to neurons in the brain. The aim of the study is to investigate immunohistochemically the neural damage that occurs in the neurons in the hippocampus regions of childhood rats, despite the protective effects of eicosapentaenoic acid EPA against the possible toxic effects of MSG and docosahexaenoic acid DHA, which is effective in brain development. Material and Method A total of 24 Wistar albino rats will be used as 6 females in childhood in each group. Group 1: Control group 0.9  saline solution 1.3.5.7.9. days subcutaneous sc, Group 2: MSG administered 4 mgg 1.3.5.7.9. days sc, Group 3: MSG  EPA administered 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, Group 4: MSG  DHA applied 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, at the end of the 9th day, the brain tissues will be placed in 10 neutral formalin for immunohistochemistry and TUNEL technique application in the hippocampus CA1 region. Results When the hippocampus CA1 region of the brains of childhood rats was examined, S100β immunoreactivity showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant p0.05. GFAP immunoreactivity also showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant both in comparison with each other and with the control group p0.05. In the MSG group, TUNEL showed a strong positive reaction in neuroglial cells. Expression was weak in the control group. TUNEL staining reaction was weak in the control group. A decrease in TUNEL staining reaction was detected in hippocampal neurons in all areas in the MSG-EPA and MSG-DHA groups. No significant difference was observed in the TUNEL staining reaction between MSGEPA and both the control group and the MSGDHA groups p0.05. Conclusion While MSG caused an increase in the positive expression of S100β, GFAP and TUNEL in the hippocampus CA1 region of the brain, MSG-EPA and MSG-DHA caused a decrease in neuronal expression and apoptosis in hippocampal neurons. Therefore, it was concluded that the use of MSG together with Omega-3 fatty acids in childhood may have a protective effect against neuroglial damage and apoptosis.

 Objective Monosodium glutamate MSG is a flavor enhancer that is added to many ready-to-eat foods and is known to be excitotoxic to neurons in the brain. The aim of the study is to investigate immunohistochemically the neural damage that occurs in the neurons in the hippocampus regions of childhood rats, despite the protective effects of eicosapentaenoic acid EPA against the possible toxic effects of MSG and docosahexaenoic acid DHA, which is effective in brain development. Material and Method A total of 24 Wistar albino rats will be used as 6 females in childhood in each group. Group 1: Control group 0.9  saline solution 1.3.5.7.9. days subcutaneous sc, Group 2: MSG administered 4 mgg 1.3.5.7.9. days sc, Group 3: MSG  EPA administered 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, Group 4: MSG  DHA applied 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, at the end of the 9th day, the brain tissues will be placed in 10 neutral formalin for immunohistochemistry and TUNEL technique application in the hippocampus CA1 region. Results When the hippocampus CA1 region of the brains of childhood rats was examined, S100β immunoreactivity showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant p0.05. GFAP immunoreactivity also showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant both in comparison with each other and with the control group p0.05. In the MSG group, TUNEL showed a strong positive reaction in neuroglial cells. Expression was weak in the control group. TUNEL staining reaction was weak in the control group. A decrease in TUNEL staining reaction was detected in hippocampal neurons in all areas in the MSG-EPA and MSG-DHA groups. No significant difference was observed in the TUNEL staining reaction between MSGEPA and both the control group and the MSGDHA groups p0.05. Conclusion While MSG caused an increase in the positive expression of S100β, GFAP and TUNEL in the hippocampus CA1 region of the brain, MSG-EPA and MSG-DHA caused a decrease in neuronal expression and apoptosis in hippocampal neurons. Therefore, it was concluded that the use of MSG together with Omega-3 fatty acids in childhood may have a protective effect against neuroglial damage and apoptosis.

 Objective Monosodium glutamate MSG is a flavor enhancer that is added to many ready-to-eat foods and is known to be excitotoxic to neurons in the brain. The aim of the study is to investigate immunohistochemically the neural damage that occurs in the neurons in the hippocampus regions of childhood rats, despite the protective effects of eicosapentaenoic acid EPA against the possible toxic effects of MSG and docosahexaenoic acid DHA, which is effective in brain development. Material and Method A total of 24 Wistar albino rats will be used as 6 females in childhood in each group. Group 1: Control group 0.9  saline solution 1.3.5.7.9. days subcutaneous sc, Group 2: MSG administered 4 mgg 1.3.5.7.9. days sc, Group 3: MSG  EPA administered 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, Group 4: MSG  DHA applied 4 mgg 1.3.5.7.9. days sc  300 mgkg 9 days orally, at the end of the 9th day, the brain tissues will be placed in 10 neutral formalin for immunohistochemistry and TUNEL technique application in the hippocampus CA1 region. Results When the hippocampus CA1 region of the brains of childhood rats was examined, S100β immunoreactivity showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant p0.05. GFAP immunoreactivity also showed a strong and significant reaction in the MSG group compared to the other groups p0.000. It was observed that the difference between MSGEPA and MSGDHA groups was insignificant both in comparison with each other and with the control group p0.05. In the MSG group, TUNEL showed a strong positive reaction in neuroglial cells. Expression was weak in the control group. TUNEL staining reaction was weak in the control group. A decrease in TUNEL staining reaction was detected in hippocampal neurons in all areas in the MSG-EPA and MSG-DHA groups. No significant difference was observed in the TUNEL staining reaction between MSGEPA and both the control group and the MSGDHA groups p0.05. Conclusion While MSG caused an increase in the positive expression of S100β, GFAP and TUNEL in the hippocampus CA1 region of the brain, MSG-EPA and MSG-DHA caused a decrease in neuronal expression and apoptosis in hippocampal neurons. Therefore, it was concluded that the use of MSG together with Omega-3 fatty acids in childhood may have a protective effect against neuroglial damage and apoptosis.

 Amaç Monosodyum glutamat MSG birçok hazır besinin içine katılan ve beyinde nöronlar üzerine eksitotoksik olduğu bilinen lezzet arttırıcıdır. Çalışmanın amacı çocukluk dönemindeki ratların hippokampus bölgelerindeki nöronlarda, MSGnın olası toksik etkisine karşı eikosapentaenoik asit EPA ve beyin gelişiminde etkili olan dokosaheksaenoik asit DHAin koruyucu etkilerine rağmen beyinde oluşan nöral hasarın immünohistokimyasal incelenmesidir. Gereç ve Yöntem Her bir grupta çocukluk döneminde olan 4 haftalık, 170-205 gr ağırlığında 6 adet, toplam 24 Wistar albino dişi ratlar kullanılacaktır. 1. Grup: Kontrol grubu 0,9  saline solution 1.3.5.7.9. günler subcutan sc verildi, 2. Grup: MSG uygulanan 4 mgg 1.3.5.7.9. günler sc, 3. Grup: MSG  EPA uygulanan 4 mgg 1.3.5.7.9. günler sc  300 mgkg 9 gün oral, 4. Grup: MSG  DHA uygulanan 4 mgg 1.3.5.7.9. günler sc  300 mgkg 9 günler oral, 9. Günün sonunda beyin dokularının hipokampus CA1 bölgesinde immünohistokimya ve TUNEL tekniği uygulaması için 10 luk nötral formalin içine alınacaktır. Bulgular Çocukluk dönemindeki dişi ratların beyinlerinin hippokampüs CA1 bölgesi incelendiğinde, S100β immunoreaktivitesi MSG grubunda diğer gruplara göre kuvvetli ve anlamlı reaksiyon gösterdi p0,000. MSGEPA ve MSGDHA grupları arasında fark anlamsız olduğu izlendi p0,05. GFAP immunoreaktivitesi yine MSG grubunda diğer gruplara göre kuvvetli ve anlamlı reaksiyon gösterdi p0,000. MSGEPA ve MSGDHA grupları hem kendi aralarında hemde kontrol grubu ile karşılaştırmalarında fark anlamsız olduğu gözlendi p0.05. MSG grubunda nöroglial hücrelerde kuvvetli TUNEL pozitif reaksiyon gösterdi. Kontrol grubunda TUNEL boyama reakasiyonu zayıftı. MSG-EPA ve MSG-DHA gruplarında tüm alanlarda hipokampal nöronlarda TUNEL boyama reaksiyonunda azalma saptandı. MSGEPA ve hem kontrol grubu arasında hem de MSGDHA grupları arasında TUNEL boyama reaksiyonunda anlamlı bir fark gözlenmedi p0,05. Sonuç MSG, beyinde hipokampus CA1 bölgesinde, S100β, GFAP ve TUNEL pozitif ekspresyonunda artışa neden olurken, MSG-EPA ve MSG-DHA hippokampal nöronlarda nöronal ekpresyonlarda ve apoptozisde azalmaya neden olmuştur. Bu nedenle çocukluk döneminde MSGnin Omega-3 yağ asitleri ile birlikte kullanımının nöroglial hasarlanma ve apoptozisten koruyucu etkisi olabileceği sonucuna varıldı.

Makale detayları

INVESTIGATION OF THE PROTECTIVE EFFECT OF OMEGA-3 FATTY ACIDS ON MONOSODIUM GLUTAMATE-INDUCED HIPPOCAMPAL INJURY

Yazarlar:

Hayrunnisa Yesil Sarsmaz | Seren Gülşen Gürgen

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.21-29

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313403

pISSN:

1300-7416

MSG

Omega-3

TUNEL

S100β

GFAPMSG

Omega-3

TUNEL

S100β

GFAP

Sayı

Makale detayları

Yazarlar: Hayrunnisa Yesil Sarsmaz | Seren Gülşen Gürgen

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.21-29

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001313403

pISSN: 1300-7416

Sayı

Yazarlar:

Hayrunnisa Yesil Sarsmaz | Seren Gülşen Gürgen

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.31 Issue 1, pp.21-29

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001313403

pISSN:

1300-7416