HPLC-DAD ANALYSIS, AND DETERMINATION OF ANTIOXIDANT, ANTICHOLINESTERASE AND ANTIDIABETIC ACTIVITIES OF Polyporus squamosus (Huds.) Fr.

Gülsen Tel-çayan

Makale detayları

HPLC-DAD ANALYSIS, AND DETERMINATION OF ANTIOXIDANT, ANTICHOLINESTERASE AND ANTIDIABETIC ACTIVITIES OF Polyporus squamosus (Huds.) Fr.

Yazarlar:

Gülsen Tel-çayan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.73-80

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305088

pISSN:

2149-3596

Anahtar kelimeler:

Özet:

 Polyporus species is a valuable species of the Polyporaceae family with defined bioactive properties among medicinal mushrooms. This study was undertaken to investigate the antioxidant, anticholinesterase and antidiabetic activities of Polyporus squamosus Huds. Fr. with characterization of phenolic profile by HPLC-DAD. Fumaric acid 190.070.08 µgg, catechin hydrate 46.960.02 µgg, ferulic acid 21.980.03 µgg, trans-cinnamic acid 16.660.02 µgg, and protocatechuic acid 13.290.06 µgg were detected as the most abundant compounds in P. squamosus by HPLC-DAD. P. squamosus methanol extract exhibited higher antioxidant activity than the hexane extract in β-carotene linoleic acid IC50: 73.750.28 µgmL, DPPH scavenging 7.560.00 inhibition at 400 µgmL, ABTS scavenging IC50: 154.300.55 µgmL, and metal chelating 35.611.20 inhibition at 400 µgmL assays. P. squamosus hexane extract was determined as the stronger inhibitor than the methanol extract against all enzymes with inhibition values of 39.480.45 on AChE at 200 µgmL, 28.021.34 on BChE at 200 µgmL, 96.700.80 on α-amylase at 1000 µgmL, and 39.930.52 on α-glucosidase at 500 µgmL. The present study highlighted the importance of P. squamosus as a promising source of valuable therapeutic compounds that could be considered as an alternatives to synthetic drugs.

 Polyporus species is a valuable species of the Polyporaceae family with defined bioactive properties among medicinal mushrooms. This study was undertaken to investigate the antioxidant, anticholinesterase and antidiabetic activities of Polyporus squamosus Huds. Fr. with characterization of phenolic profile by HPLC-DAD. Fumaric acid 190.070.08 µgg, catechin hydrate 46.960.02 µgg, ferulic acid 21.980.03 µgg, trans-cinnamic acid 16.660.02 µgg, and protocatechuic acid 13.290.06 µgg were detected as the most abundant compounds in P. squamosus by HPLC-DAD. P. squamosus methanol extract exhibited higher antioxidant activity than the hexane extract in β-carotene linoleic acid IC50: 73.750.28 µgmL, DPPH scavenging 7.560.00 inhibition at 400 µgmL, ABTS scavenging IC50: 154.300.55 µgmL, and metal chelating 35.611.20 inhibition at 400 µgmL assays. P. squamosus hexane extract was determined as the stronger inhibitor than the methanol extract against all enzymes with inhibition values of 39.480.45 on AChE at 200 µgmL, 28.021.34 on BChE at 200 µgmL, 96.700.80 on α-amylase at 1000 µgmL, and 39.930.52 on α-glucosidase at 500 µgmL. The present study highlighted the importance of P. squamosus as a promising source of valuable therapeutic compounds that could be considered as an alternatives to synthetic drugs.

 Polyporus species is a valuable species of the Polyporaceae family with defined bioactive properties among medicinal mushrooms. This study was undertaken to investigate the antioxidant, anticholinesterase and antidiabetic activities of Polyporus squamosus Huds. Fr. with characterization of phenolic profile by HPLC-DAD. Fumaric acid 190.070.08 µgg, catechin hydrate 46.960.02 µgg, ferulic acid 21.980.03 µgg, trans-cinnamic acid 16.660.02 µgg, and protocatechuic acid 13.290.06 µgg were detected as the most abundant compounds in P. squamosus by HPLC-DAD. P. squamosus methanol extract exhibited higher antioxidant activity than the hexane extract in β-carotene linoleic acid IC50: 73.750.28 µgmL, DPPH scavenging 7.560.00 inhibition at 400 µgmL, ABTS scavenging IC50: 154.300.55 µgmL, and metal chelating 35.611.20 inhibition at 400 µgmL assays. P. squamosus hexane extract was determined as the stronger inhibitor than the methanol extract against all enzymes with inhibition values of 39.480.45 on AChE at 200 µgmL, 28.021.34 on BChE at 200 µgmL, 96.700.80 on α-amylase at 1000 µgmL, and 39.930.52 on α-glucosidase at 500 µgmL. The present study highlighted the importance of P. squamosus as a promising source of valuable therapeutic compounds that could be considered as an alternatives to synthetic drugs.

 Polyporus türleri, tıbbi mantarlar arasında tanımlanan biyoaktif özellikleri ile Polyporaceae familyasının değerli bir türüdür. Bu çalışma, Polyporus squamosus Huds. Fr. mantarının antioksidan, antikolinesteraz ve antidiyabetik aktiviteleri ile HPLC-DAD ile fenolik profilinin karakterizasyonu araştırmak için yapılmıştır. Fumarik asit 190,070,08 µgg, kateşin hidrat 46,960,02 µgg, ferulik asit 21,980,03 µgg, trans-sinnamik asit 16,660,02 µgg ve protokateşik asit 13,290,06 µgg HPLC-DAD ile P. squamosusta en bol bulunan bileşikler olarak tespit edilmiştir. P. squamosus metanol ekstresi β-karoten linoleik asit IC50: 73,750,28 µgmL, DPPH giderim 400 µgmLde 7,560,00 inhibisyon, ABTS giderim IC50: 154,300,55 µgmL ve metal kelatlama 400 µgmLde 35,611,20 inhibisyon yöntemlerinde hekzan ekstresinden daha yüksek antioksidan aktivite göstermiştir. P. squamosus hekzan ekstresi AChE enzimine karşı 200 µgmLde 39,480,45, BChE enzimine karşı 200 µgmLde 28,021,34, α-amilaz enzimine karşı 1000 µgmLde 96,700,80 ve α-glukozidaz enzimine karşı 500 µgmLde 39,930,52 inhibisyon değerleri ile metanol ekstresinden daha güçlü bir inhibitör olarak belirlenmiştir. Mevcut çalışma, P. squamosusun sentetik ilaçlara alternatif olarak değerlendirilebilecek değerli terapötik bileşiklerin umut verici bir kaynağı olarak önemini vurgulamıştır.

Makale detayları

HPLC-DAD ANALYSIS, AND DETERMINATION OF ANTIOXIDANT, ANTICHOLINESTERASE AND ANTIDIABETIC ACTIVITIES OF Polyporus squamosus (Huds.) Fr.

Yazarlar:

Gülsen Tel-çayan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.73-80

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305088

pISSN:

2149-3596

Polyporus squamosus

Phenolic profile

HPLC-DAD

Antioxidant activity

Anticholinesterase activity

Antidiabetic activityPolyporus squamosus

Fenolik profil

HPLC-DAD

Antioksidan aktivite

Antikolinesteraz aktivite

Antidiyabetik aktivite

Sayı

Makale detayları

Yazarlar: Gülsen Tel-çayan

Yayıncı: Mugla Sitki Kocman University

Ana Başlıklar: Doğal Ürünler ve Biyoaktif Bileşikler

Dergi: Mugla Journal of Science and Technology Vol.9 Issue 2, pp.73-80

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001305088

pISSN: 2149-3596

Sayı

Yazarlar:

Gülsen Tel-çayan

Yayıncı:

Mugla Sitki Kocman University

Ana Başlıklar:

Doğal Ürünler ve Biyoaktif Bileşikler

Dergi:

Mugla Journal of Science and Technology Vol.9 Issue 2, pp.73-80

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001305088

pISSN:

2149-3596