An Efficient DNA Isolation Method from Nigella sativa L. (Ranunculaceae) Seeds for RAPD and ISSR Analysis

İsmail POYRAZ

Makale detayları

An Efficient DNA Isolation Method from Nigella sativa L. (Ranunculaceae) Seeds for RAPD and ISSR Analysis

Yazarlar:

İsmail POYRAZ

Yayıncı:

Bilecik Seyh Edebali University

Dergi:

Bilecik Seyh Edebali University Journal of Science 2014 Vol.1 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

6/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000891961

Sıklık:

İki Yıllık

eISSN:

2458-7575

Özet:

 

Nigella sativa L. (Ranunculaceae) seeds (black cumin) that contain high oil, terpene, protein, alkaloids are an economically important for medicine and food industry. Lack of an efficient DNA isolation procedure is a limiting factor for any molecular studies of these plant seeds. We have used a genomic DNA isolation protocol for Nigella sativa seeds based on a hexadecyltrimethylammonium bromide (CTAB) method described for other plant species. The method is a modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone (PVP), and successive isoamyl alcohol chloroform extractions and involves mortar grinding of black seeds. Black seeds include much little DNA despite high amounts nutrients as polysaccharides and oils. Therefore, the yield was approx., 20 ng DNA per 150 mg of dry seed material. The genomic DNA obtained by this method was suitable to be used in inter-simple sequence repeat (ISSR) and random amplified-polymorphic DNA (RAPD) reactions. This extraction method should facilitate the molecular analysis as genotypic selection via use of dry seeds instead of germination of N. sativa seeds.

Makale detayları

An Efficient DNA Isolation Method from Nigella sativa L. (Ranunculaceae) Seeds for RAPD and ISSR Analysis

Yazarlar: İsmail POYRAZ

Yayıncı: Bilecik Seyh Edebali University

Dergi: Bilecik Seyh Edebali University Journal of Science 2014 Vol.1 Issue 1, pp.22-27

Belge Tipi: Makale

Yayın Tarihi: 6/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000891961

Sıklık: İki Yıllık

eISSN: 2458-7575

Sayı

Yazarlar:

İsmail POYRAZ

Yayıncı:

Bilecik Seyh Edebali University

Dergi:

Bilecik Seyh Edebali University Journal of Science 2014 Vol.1 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

6/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000891961

Sıklık:

İki Yıllık

eISSN:

2458-7575