Investigation of nasal Staphylococcus aureus carriage by real-time PCR in patients receiving hemodialysis treatment

Songül Çetik Yıldız; Cemil Demir

Makale detayları

Investigation of nasal Staphylococcus aureus carriage by real-time PCR in patients receiving hemodialysis treatment

Yazarlar:

Songül Çetik Yıldız | Cemil Demir

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Infectious Diseases

Dergi:

The European Research Journal Vol.9 Issue 2, pp.277-284

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282998

pISSN:

2149-3189

Anahtar kelimeler:

Özet:

 Objectives: Staphylococcus aureus , coagulase-negative staphylococci CoNS, and methicillin-resistant S. aureus MRSA, which are significant nosocomial pathogens, have become a growing global problem because their carriage and diseases have become resistant to many antibiotics. This study aimed to investigate and determine the rate of MRSA carriage among patients receiving hemodialysis treatment using molecular methods. Methods: In the 254 hemodialysis patients, the nasal carriage rates, susceptibility and resistance to S. aureus , CoNS and MRSA were examined using culture and real-time PCR methods. Nasal samples from hemodialysis patients were examined using real-time PCR. Microscopic examination was performed using the Gram staining method, and S. aureus was identified using catalase and coagulase. The strains were then tested for antibiotic susceptibility. Staphylococci was isolated from 231 of the 254 patients. Results: S. aureus carriage was detected in 50 patients, MRSA in 16, methicillin-susceptible S. aureus MSSA in 33, CoNS in 66, methicillin-resistant CoNS MR-CoNS in 38, and methicillin-susceptible CoNS MS-CoNS in 28. S. aureus and MRSA strains exhibited 100 susceptibility to nitrofurantoin, and vancomycin. MSSA strains showed the highest susceptibility to chloramphenicol, clindamycin 84.8, and co-trimoxazole 36.4. CoNS showed 100 susceptibility to vancomycin, and 16.7 susceptibility to ampicillin. Vancomycin was found to be the most effective antibiotic against S. aureus , CoNS, and MRSA pathogens identified in patients undergoing hemodialysis, whereas penicillin resistance was found. Conclusions: It can be concluded that one of the most effective ways to prevent the formation of antibiotic resistant strains is the hygiene of the hospital and hospital staff. Real-time PCR is very important for analyzing with high sensitivity.

 Objectives: Staphylococcus aureus , coagulase-negative staphylococci CoNS, and methicillin-resistant S. aureus MRSA, which are significant nosocomial pathogens, have become a growing global problem because their carriage and diseases have become resistant to many antibiotics. This study aimed to investigate and determine the rate of MRSA carriage among patients receiving hemodialysis treatment using molecular methods. Methods: In the 254 hemodialysis patients, the nasal carriage rates, susceptibility and resistance to S. aureus , CoNS and MRSA were examined using culture and real-time PCR methods. Nasal samples from hemodialysis patients were examined using real-time PCR. Microscopic examination was performed using the Gram staining method, and S. aureus was identified using catalase and coagulase. The strains were then tested for antibiotic susceptibility. Staphylococci was isolated from 231 of the 254 patients. Results: S. aureus carriage was detected in 50 patients, MRSA in 16, methicillin-susceptible S. aureus MSSA in 33, CoNS in 66, methicillin-resistant CoNS MR-CoNS in 38, and methicillin-susceptible CoNS MS-CoNS in 28. S. aureus and MRSA strains exhibited 100 susceptibility to nitrofurantoin, and vancomycin. MSSA strains showed the highest susceptibility to chloramphenicol, clindamycin 84.8, and co-trimoxazole 36.4. CoNS showed 100 susceptibility to vancomycin, and 16.7 susceptibility to ampicillin. Vancomycin was found to be the most effective antibiotic against S. aureus , CoNS, and MRSA pathogens identified in patients undergoing hemodialysis, whereas penicillin resistance was found. Conclusions: It can be concluded that one of the most effective ways to prevent the formation of antibiotic resistant strains is the hygiene of the hospital and hospital staff. Real-time PCR is very important for analyzing with high sensitivity.

 Objectives: Staphylococcus aureus , coagulase-negative staphylococci CoNS, and methicillin-resistant S. aureus MRSA, which are significant nosocomial pathogens, have become a growing global problem because their carriage and diseases have become resistant to many antibiotics. This study aimed to investigate and determine the rate of MRSA carriage among patients receiving hemodialysis treatment using molecular methods. Methods: In the 254 hemodialysis patients, the nasal carriage rates, susceptibility and resistance to S. aureus , CoNS and MRSA were examined using culture and real-time PCR methods. Nasal samples from hemodialysis patients were examined using real-time PCR. Microscopic examination was performed using the Gram staining method, and S. aureus was identified using catalase and coagulase. The strains were then tested for antibiotic susceptibility. Staphylococci was isolated from 231 of the 254 patients. Results: S. aureus carriage was detected in 50 patients, MRSA in 16, methicillin-susceptible S. aureus MSSA in 33, CoNS in 66, methicillin-resistant CoNS MR-CoNS in 38, and methicillin-susceptible CoNS MS-CoNS in 28. S. aureus and MRSA strains exhibited 100 susceptibility to nitrofurantoin, and vancomycin. MSSA strains showed the highest susceptibility to chloramphenicol, clindamycin 84.8, and co-trimoxazole 36.4. CoNS showed 100 susceptibility to vancomycin, and 16.7 susceptibility to ampicillin. Vancomycin was found to be the most effective antibiotic against S. aureus , CoNS, and MRSA pathogens identified in patients undergoing hemodialysis, whereas penicillin resistance was found. Conclusions: It can be concluded that one of the most effective ways to prevent the formation of antibiotic resistant strains is the hygiene of the hospital and hospital staff. Real-time PCR is very important for analyzing with high sensitivity.

 Objectives: Staphylococcus aureus , coagulase-negative staphylococci CoNS, and methicillin-resistant S. aureus MRSA, which are significant nosocomial pathogens, have become a growing global problem because their carriage and diseases have become resistant to many antibiotics. This study aimed to investigate and determine the rate of MRSA carriage among patients receiving hemodialysis treatment using molecular methods. Methods: In the 254 hemodialysis patients, the nasal carriage rates, susceptibility and resistance to S. aureus , CoNS and MRSA were examined using culture and real-time PCR methods. Nasal samples from hemodialysis patients were examined using real-time PCR. Microscopic examination was performed using the Gram staining method, and S. aureus was identified using catalase and coagulase. The strains were then tested for antibiotic susceptibility. Staphylococci was isolated from 231 of the 254 patients. Results: S. aureus carriage was detected in 50 patients, MRSA in 16, methicillin-susceptible S. aureus MSSA in 33, CoNS in 66, methicillin-resistant CoNS MR-CoNS in 38, and methicillin-susceptible CoNS MS-CoNS in 28. S. aureus and MRSA strains exhibited 100 susceptibility to nitrofurantoin, and vancomycin. MSSA strains showed the highest susceptibility to chloramphenicol, clindamycin 84.8, and co-trimoxazole 36.4. CoNS showed 100 susceptibility to vancomycin, and 16.7 susceptibility to ampicillin. Vancomycin was found to be the most effective antibiotic against S. aureus , CoNS, and MRSA pathogens identified in patients undergoing hemodialysis, whereas penicillin resistance was found. Conclusions: It can be concluded that one of the most effective ways to prevent the formation of antibiotic resistant strains is the hygiene of the hospital and hospital staff. Real-time PCR is very important for analyzing with high sensitivity.

Makale detayları

Investigation of nasal Staphylococcus aureus carriage by real-time PCR in patients receiving hemodialysis treatment

Yazarlar:

Songül Çetik Yıldız | Cemil Demir

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Infectious Diseases

Dergi:

The European Research Journal Vol.9 Issue 2, pp.277-284

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282998

pISSN:

2149-3189

Staphylococcus aureus

nasal carriage

antibiotic resistant

infection

real-time PCR

Sayı

Makale detayları

Investigation of nasal Staphylococcus aureus carriage by real-time PCR in patients receiving hemodialysis treatment

Yazarlar: Songül Çetik Yıldız | Cemil Demir

Yayıncı: Prusa Medical Publishing

Ana Başlıklar: Infectious Diseases

Dergi: The European Research Journal Vol.9 Issue 2, pp.277-284

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282998

pISSN: 2149-3189

Staphylococcus aureus

nasal carriage

antibiotic resistant

infection

real-time PCR

Sayı

Yazarlar:

Songül Çetik Yıldız | Cemil Demir

Yayıncı:

Prusa Medical Publishing

Ana Başlıklar:

Infectious Diseases

Dergi:

The European Research Journal Vol.9 Issue 2, pp.277-284

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282998

pISSN:

2149-3189