Reduction of Lignin in Tobacco Through the Expression of an Antisense Caffeic Acid O-methyltransferase

Tijen Talas OĞRAŞ

Makale detayları

Reduction of Lignin in Tobacco Through the Expression of an Antisense Caffeic Acid O-methyltransferase

Yazarlar:

Tijen Talas OĞRAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2000 Vol.24 Issue 4, pp.221-226

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000724619

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

Caffeic acid O-methyltransferase (COMT) is a methylation enzyme involved in the early stages of lignin biosynthesis. To  examine the effect of antisense expression of a heterologous COMT gene on lignification, an antisense construct of a cDNA encoding  a caffeic acid O-methyltransferase ( pOMT8) from Stylosanthes humilis Humb., Bonpl. & Kunth Fabaceae was transferred into  tobacco via Agrobacterium tumefaciens. A large number of kanamycin-resistant shoots were recovered after transformation, and  randomly selected putative transgenics were analysed for stable integration of the transgene into their genomes. The analyses  demonstrated that antisense expression of pOMT8 resulted in reductions in total COMT activity, which was correlated with reduction  in the lignin content of the transgenic plants. The amount of lignin in one of the transgenic lines decreased by 62% compared with  untransformed control plants.

 

Caffeic acid O-methyltransferase (COMT) is a methylation enzyme involved in the early stages of lignin biosynthesis. To  examine the effect of antisense expression of a heterologous COMT gene on lignification, an antisense construct of a cDNA encoding  a caffeic acid O-methyltransferase ( pOMT8) from Stylosanthes humilis Humb., Bonpl. & Kunth Fabaceae was transferred into  tobacco via Agrobacterium tumefaciens. A large number of kanamycin-resistant shoots were recovered after transformation, and  randomly selected putative transgenics were analysed for stable integration of the transgene into their genomes. The analyses  demonstrated that antisense expression of pOMT8 resulted in reductions in total COMT activity, which was correlated with reduction  in the lignin content of the transgenic plants. The amount of lignin in one of the transgenic lines decreased by 62% compared with  untransformed control plants.

Makale detayları

Reduction of Lignin in Tobacco Through the Expression of an Antisense Caffeic Acid O-methyltransferase

Yazarlar: Tijen Talas OĞRAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2000 Vol.24 Issue 4, pp.221-226

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000724619

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Tijen Talas OĞRAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2000 Vol.24 Issue 4, pp.221-226

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000724619

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X