Comparison of the in vitro anticancer effect of habanero pepper extract containing  capsaicin with that of pure capsaicin in selected dog neoplastic cell lines

łukasz ADASZEK; Olga SŁABCZYŃSKA; Paweł ŁYP; Dorota RÓŻAŃSKA; Jerzy ZIĘTEK; Maciej ORZELSKI; Anna ŚMIECH; Marta STANIEC; Dorota KRASUCKA; Stanisław WINIARCZYK

Makale detayları

Comparison of the in vitro anticancer effect of habanero pepper extract containing capsaicin with that of pure capsaicin in selected dog neoplastic cell lines

Yazarlar:

łukasz ADASZEK | Olga SŁABCZYŃSKA | Paweł ŁYP | Dorota RÓŻAŃSKA | Jerzy ZIĘTEK | Maciej ORZELSKI | Anna ŚMIECH | Marta STANIEC | Dorota KRASUCKA | Stanisław WINIARCZYK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.243-250

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764566

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer 
lines,  DAN  (fibroblasts  isolated  from  osteosarcoma)  and  D-17  (epithelium  cells  of  osteosarcoma  obtained  from  pulmonary  metastatic  
tumors), under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 
cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation (37 °C, 5% CO2), the fluid above the culture was removed, and diluted 
habanero pepper extract was added. The concentrations of capsaicin in the extract were 10, 20, 50, 100, 150, and 200 ?M (in the culture 
fluid with 10% of FBS). The cells were incubated for 24?96 h (37 °C, 5% CO2). After the incubation period, cytotoxicity assessment of the 
habanero pepper extract and cell proliferation impairment by the habanero pepper extract in a suspension of cell lines, in MTT test, as well 
as the cytometric examination of viability of cancer cells exposed to the habanero pepper extract were performed. The results indicate that 
the extract shows better anticarcinogenic activity in vitro compared with pure capsaicin. Confirmation of the anticancer effectiveness of 
the extract on the cells is a starting point for wide clinical observations and a good indication for further research.

Makale detayları

Comparison of the in vitro anticancer effect of habanero pepper extract containing capsaicin with that of pure capsaicin in selected dog neoplastic cell lines

Yazarlar: łukasz ADASZEK | Olga SŁABCZYŃSKA | Paweł ŁYP | Dorota RÓŻAŃSKA | Jerzy ZIĘTEK | Maciej ORZELSKI | Anna ŚMIECH | Marta STANIEC | Dorota KRASUCKA | Stanisław WINIARCZYK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.243-250

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000764566

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

łukasz ADASZEK | Olga SŁABCZYŃSKA | Paweł ŁYP | Dorota RÓŻAŃSKA | Jerzy ZIĘTEK | Maciej ORZELSKI | Anna ŚMIECH | Marta STANIEC | Dorota KRASUCKA | Stanisław WINIARCZYK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.243-250

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764566

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128