Construction of an oxygen detection-based optic laccase biosensor for polyphenolic compound detection

Kadir BİLİR; Marie-theres WEIL; Julia LOCHEAD; Fatma Neşe KÖK; Tobias WERNER

Makale detayları

Construction of an oxygen detection-based optic laccase biosensor for polyphenolic compound detection

Yazarlar:

Kadir BİLİR | Marie-theres WEIL | Julia LOCHEAD | Fatma Neşe KÖK | Tobias WERNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1303-1310

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811546

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

A fiber optic biosensor was constructed from Pleurotus ostreatus laccase for the detection of polyphenolic compounds. Laccase was immobilized on the surface of the commercially available fiber optic oxygen sensor spots by using 3-aminopropylsilanetriol, glutaraldehyde, and amino-modified carboxycellulose. A diffusion layer containing tetramethyl orthosilicate (TMOS), trimethoxymethylsilane (Tri-MOS), and polyvinyl alcohol (PVA) was added to the immobilized laccase layer. The consumption of oxygen as a result of laccase activity was monitored using a fiber optical measuring setup with catechol as a model substrate. The optimal enzyme amount was determined as 1.5 mg per 50 µL of enzyme layer mixture, and with one diffusion layer and at pH 6.9, optimum detection conditions were attained. The biosensors have high reproducibility, stability (at least 85 days if stored in PBS at 4 °C), and convenient measurement duration (ca. 25 min between two successive measurements). The biosensor was found to have a broad linear working range for catechol (40-600 μM) and to be applicable to a flow-through system. In summary, an easy-to-produce, reproducible, and stable laccase sensor with a broad linear working range was produced. The sensor has potential in the food industry as well as in environmental monitoring for the detection of phenolic compounds.

Makale detayları

Construction of an oxygen detection-based optic laccase biosensor for polyphenolic compound detection

Yazarlar: Kadir BİLİR | Marie-theres WEIL | Julia LOCHEAD | Fatma Neşe KÖK | Tobias WERNER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1303-1310

Belge Tipi: Makale

Yayın Tarihi: 11/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811546

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Kadir BİLİR | Marie-theres WEIL | Julia LOCHEAD | Fatma Neşe KÖK | Tobias WERNER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1303-1310

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811546

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152