TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Laid Ziane; Abdelaziz Berreghioua

Makale detayları

TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Yazarlar:

Laid Ziane | Abdelaziz Berreghioua

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), pp.1313-1317

Belge Tipi:

Makale

Yayın Tarihi:

11/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312155

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents. Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plants antioxidant activity, were carried out using the conventional procedures. Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent GAEg dry extract and 439 mg quercetin equivalent QEg dry extract. The hydro-alcoholic extract was extracted by liquidliquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mgml, respectively.

 Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents. Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plants antioxidant activity, were carried out using the conventional procedures. Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent GAEg dry extract and 439 mg quercetin equivalent QEg dry extract. The hydro-alcoholic extract was extracted by liquidliquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mgml, respectively.

 Objective: This study sought to identify potential sources for upcoming novel antioxidants in food and pharmaceutical formulations by screening various solvent extracts from the leaves of Atriplex halimus Lin. for their ability to exhibit strong antioxidant activity in vitro, as well as their total phenolic and flavonoid contents. Material and Method: To determine the total amount of polyphenols and flavonoids in Atriplex halimus extracts, including ethyl ether, ethyl acetate, and n-butanol extracts, as well as their corresponding impact on this plants antioxidant activity, were carried out using the conventional procedures. Result and Discussion: In the current investigation, total phenolic and flavonoid contents in butanolic extract were found to be 68.20 mg gallic acid equivalent GAEg dry extract and 439 mg quercetin equivalent QEg dry extract. The hydro-alcoholic extract was extracted by liquidliquid partition with solvents of increasing polarity: ethyl ether, ethyl acetate and n-butanol by the free radical DPPH removing garbage and HPTLC as well as their reduction kinetics. It was found that the extract of butanol and ethyl acetate had powerful uplifting power garbage DPPH with IC50 values of 2.1959 and 2.4234 mgml, respectively.

 Amaç: Bu çalışmada, Atriplex halimus bitkisinin tamamının çeşitli solvent ekstrelerini, in vitro güçlü antioksidan aktivite sergileme yetenekleri ve ayrıca toplam fenolik ve flavonoid içerikleri açısından tarayarak, gıda ve farmasötik formülasyonlarda gelecek yeni antioksidanlar için potansiyel kaynakları belirleme amaçlandı. Gereç ve Yöntem: Etil eter, etil asetat ve n-butanol ekstreleri dahil olmak üzere Atripleks halimus ekstrelerindeki polifenollerin ve flavonoidlerin toplam miktarının yanı sıra bunların bu bitkinin antioksidan aktivitesi üzerindeki karşılık gelen etkilerini belirlemek için geleneksel prosedürler kullanılarak gerçekleştirildi. Sonuç ve Tartışma: Mevcut araştırmada, bütanolik ekstredeki toplam fenolik ve flavonoid içeriğinin 68.20 mg gallik asit eşdeğeri GAEg kuru ekstrakt ve 439 mg kersetin eşdeğeri QEg kuru ekstre olduğu bulunmuştur. Hidro-alkolik ekstresi, artan polariteye sahip solventler etil eter, etil asetat ve n-butanol ile sıvısıvı partitisyona tabi tutulmasıyla, serbest radikal DPPHnin atıkları ve HPTLCyi ve bunların indirgeme kinetiklerini ortadan kaldırmasıyla ekstre edildi. Butanol ve etil asetat ekstreleri sırasıyla 2.1959 ve 2.4234 mgml IC50 değerleriyle DPPH radikali süpürücü aktivitesine sahip olduğu bulundu.

Makale detayları

TOTAL PHENOLIC AND FLAVONOIDS QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF BIOACTIVE EXTRACTS FROM THE LEAVES OF ATRIPLEX HALIMUS

Yazarlar:

Laid Ziane | Abdelaziz Berreghioua

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), pp.1313-1317

Belge Tipi:

Makale

Yayın Tarihi:

11/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312155

pISSN:

1015-3918

Antioxidant activity

Atriplex halimus

bioactive extract

DPPH

phytochemical

quercetinAntioksidan aktivite

Atriplex halimus

biyoaktif ekstre

DPPH

fitokimyasal

quercetin

Sayı

Makale detayları

Yazarlar: Laid Ziane | Abdelaziz Berreghioua

Yayıncı: Ankara University

Ana Başlıklar: Eczacılıkta Analitik Kimya

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), pp.1313-1317

Belge Tipi: Makale

Yayın Tarihi: 11/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001312155

pISSN: 1015-3918

Sayı

Yazarlar:

Laid Ziane | Abdelaziz Berreghioua

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 4-1st international Conference on Trends Methods in Analytical Chemistry (CTMAC 2023), pp.1313-1317

Belge Tipi:

Makale

Yayın Tarihi:

11/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001312155

pISSN:

1015-3918