Random Amplified Polymorphic DNA (RAPD) Markers

Fevzi BARDAKCI

Makale detayları

Random Amplified Polymorphic DNA (RAPD) Markers

Yazarlar:

Fevzi BARDAKCI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 2, pp.185-196

Belge Tipi:

Makale

Yayın Tarihi:

1/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813866

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplification products of anonymous DNA sequences using single, short and arbitrary oligonucleotide primers, and thus do not require prior knowledge of a DNA sequence. Low expense, efficiency in developinga large number of DNA markers in a short time and requirement for less sophisticated equipment has made the RAPD technique valuable although reproducibility of the RAPD profile is still the centre of debate.

 

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplification products of anonymous DNA sequences using single, short and arbitrary oligonucleotide primers, and thus do not require prior knowledge of a DNA sequence. Low expense, efficiency in developinga large number of DNA markers in a short time and requirement for less sophisticated equipment has made the RAPD technique valuable although reproducibility of the RAPD profile is still the centre of debate.

Makale detayları

Random Amplified Polymorphic DNA (RAPD) Markers

Yazarlar: Fevzi BARDAKCI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2001 Vol.25 Issue 2, pp.185-196

Belge Tipi: Makale

Yayın Tarihi: 1/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000813866

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

RAPD

PCR

DNA

polymorphism

Sayı

Yazarlar:

Fevzi BARDAKCI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 2, pp.185-196

Belge Tipi:

Makale

Yayın Tarihi:

1/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813866

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152