COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Ece Battaloğlu; Zehra Gül Morçimen; Aylin Şendemir

Makale detayları

COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Yazarlar:

Ece Battaloğlu | Zehra Gül Morçimen | Aylin Şendemir

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 4, pp.327-340

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293438

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone PCL, hyaluronic acid HA coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent IF staining was performed with glial fibrillary acidic protein GFAP antibody to determine the level of gliosis. It was found that 5µgmL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

 Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone PCL, hyaluronic acid HA coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent IF staining was performed with glial fibrillary acidic protein GFAP antibody to determine the level of gliosis. It was found that 5µgmL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

 Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone PCL, hyaluronic acid HA coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent IF staining was performed with glial fibrillary acidic protein GFAP antibody to determine the level of gliosis. It was found that 5µgmL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

 Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone PCL, hyaluronic acid HA coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent IF staining was performed with glial fibrillary acidic protein GFAP antibody to determine the level of gliosis. It was found that 5µgmL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

Makale detayları

COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Yazarlar:

Ece Battaloğlu | Zehra Gül Morçimen | Aylin Şendemir

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 4, pp.327-340

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293438

pISSN:

2687-475X

Gliosis

neural tissue engineering

electrospinning

glio-protectiveGliosis

neural tissue engineering

electrospinning

glio-protective

Sayı

Makale detayları

Yazarlar: Ece Battaloğlu | Zehra Gül Morçimen | Aylin Şendemir

Yayıncı: Hacettepe University

Ana Başlıklar: Engineering

Dergi: Hacettepe Journal of Biology and Chemistry Vol.51 Issue 4, pp.327-340

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293438

pISSN: 2687-475X

Sayı

Yazarlar:

Ece Battaloğlu | Zehra Gül Morçimen | Aylin Şendemir

Yayıncı:

Hacettepe University

Ana Başlıklar:

Engineering

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.51 Issue 4, pp.327-340

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293438

pISSN:

2687-475X