A Highly Sensitive Electrochemical Immunosensor for the detection of CCR4 Biomarker with Star-Shaped Polymer Covered Disposable Electrode

Muhammet Aydın

Makale detayları

A Highly Sensitive Electrochemical Immunosensor for the detection of CCR4 Biomarker with Star-Shaped Polymer Covered Disposable Electrode

Yazarlar:

Muhammet Aydın

Yayıncı:

Hacettepe University

Ana Başlıklar:

Electroanalytical Chemistry | Sensor Technology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 3, pp.29-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349658

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 An electrochemical immunosensor prepared with the use of star-shaped polymer StrPolGMA as a biosensor matrix material was manufactured to quantify the CC chemokine receptor 4 CCR4 molecules in human serum. Various characterization techniques, including electrochemical impedance spectroscopy EIS, cyclic voltammetry CV, and scanning electron microscopy SEM, were used for the analysis of the fabrication steps of the proposed system. The impedance and CV analyses at each modification step of the manufacturing process illustrated the charge transport kinetics of the ferri-ferro redox solution. The binding of anti-CCR4 antibody and the related antibody-its CCR4 antigen biointeraction on the working electrode surface changed the interfacial electron transport. The interactions of anti-CCR4 with different levels of CCR4 were further followed via variations in impedance responses. The results illustrated that the electron transport resistance rose linearly with rising amounts of CCR4 antigen. The dynamic range and detection limit LOD were determined as 0.115-23 pgmL and 34.9 fgmL, respectively. The specificity, regeneration, storage stability, and reproducibility tests were also performed. Furthermore, CCR4 detection in the presence of interfering biomarkers was accomplished with success using the biosensing approach. The results of these studies illustrate that the introduced biosensor was a useful tool for monitoring low concentrations of CCR4 in human sera.

 An electrochemical immunosensor prepared with the use of star-shaped polymer StrPolGMA as a biosensor matrix material was manufactured to quantify the CC chemokine receptor 4 CCR4 molecules in human serum. Various characterization techniques, including electrochemical impedance spectroscopy EIS, cyclic voltammetry CV, and scanning electron microscopy SEM, were used for the analysis of the fabrication steps of the proposed system. The impedance and CV analyses at each modification step of the manufacturing process illustrated the charge transport kinetics of the ferri-ferro redox solution. The binding of anti-CCR4 antibody and the related antibody-its CCR4 antigen biointeraction on the working electrode surface changed the interfacial electron transport. The interactions of anti-CCR4 with different levels of CCR4 were further followed via variations in impedance responses. The results illustrated that the electron transport resistance rose linearly with rising amounts of CCR4 antigen. The dynamic range and detection limit LOD were determined as 0.115-23 pgmL and 34.9 fgmL, respectively. The specificity, regeneration, storage stability, and reproducibility tests were also performed. Furthermore, CCR4 detection in the presence of interfering biomarkers was accomplished with success using the biosensing approach. The results of these studies illustrate that the introduced biosensor was a useful tool for monitoring low concentrations of CCR4 in human sera.

 An electrochemical immunosensor prepared with the use of star-shaped polymer StrPolGMA as a biosensor matrix material was manufactured to quantify the CC chemokine receptor 4 CCR4 molecules in human serum. Various characterization techniques, including electrochemical impedance spectroscopy EIS, cyclic voltammetry CV, and scanning electron microscopy SEM, were used for the analysis of the fabrication steps of the proposed system. The impedance and CV analyses at each modification step of the manufacturing process illustrated the charge transport kinetics of the ferri-ferro redox solution. The binding of anti-CCR4 antibody and the related antibody-its CCR4 antigen biointeraction on the working electrode surface changed the interfacial electron transport. The interactions of anti-CCR4 with different levels of CCR4 were further followed via variations in impedance responses. The results illustrated that the electron transport resistance rose linearly with rising amounts of CCR4 antigen. The dynamic range and detection limit LOD were determined as 0.115-23 pgmL and 34.9 fgmL, respectively. The specificity, regeneration, storage stability, and reproducibility tests were also performed. Furthermore, CCR4 detection in the presence of interfering biomarkers was accomplished with success using the biosensing approach. The results of these studies illustrate that the introduced biosensor was a useful tool for monitoring low concentrations of CCR4 in human sera.

 An electrochemical immunosensor prepared with the use of star-shaped polymer StrPolGMA as a biosensor matrix material was manufactured to quantify the CC chemokine receptor 4 CCR4 molecules in human serum. Various characterization techniques, including electrochemical impedance spectroscopy EIS, cyclic voltammetry CV, and scanning electron microscopy SEM, were used for the analysis of the fabrication steps of the proposed system. The impedance and CV analyses at each modification step of the manufacturing process illustrated the charge transport kinetics of the ferri-ferro redox solution. The binding of anti-CCR4 antibody and the related antibody-its CCR4 antigen biointeraction on the working electrode surface changed the interfacial electron transport. The interactions of anti-CCR4 with different levels of CCR4 were further followed via variations in impedance responses. The results illustrated that the electron transport resistance rose linearly with rising amounts of CCR4 antigen. The dynamic range and detection limit LOD were determined as 0.115-23 pgmL and 34.9 fgmL, respectively. The specificity, regeneration, storage stability, and reproducibility tests were also performed. Furthermore, CCR4 detection in the presence of interfering biomarkers was accomplished with success using the biosensing approach. The results of these studies illustrate that the introduced biosensor was a useful tool for monitoring low concentrations of CCR4 in human sera.

Makale detayları

A Highly Sensitive Electrochemical Immunosensor for the detection of CCR4 Biomarker with Star-Shaped Polymer Covered Disposable Electrode

Yazarlar:

Muhammet Aydın

Yayıncı:

Hacettepe University

Ana Başlıklar:

Electroanalytical Chemistry | Sensor Technology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 3, pp.29-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349658

pISSN:

2687-475X

CC chemokines receptor 4

prostate cancer

disposable biosensor

Sayı

Makale detayları

A Highly Sensitive Electrochemical Immunosensor for the detection of CCR4 Biomarker with Star-Shaped Polymer Covered Disposable Electrode

Yazarlar: Muhammet Aydın

Yayıncı: Hacettepe University

Ana Başlıklar: Electroanalytical Chemistry | Sensor Technology

Dergi: Hacettepe Journal of Biology and Chemistry Vol.53 Issue 3, pp.29-42

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001349658

pISSN: 2687-475X

CC chemokines receptor 4

prostate cancer

disposable biosensor

Sayı

Yazarlar:

Muhammet Aydın

Yayıncı:

Hacettepe University

Ana Başlıklar:

Electroanalytical Chemistry | Sensor Technology

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 3, pp.29-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001349658

pISSN:

2687-475X