Predominant Inflammatory and Th1 biased cytokine secretion pre- and post- kidney transplantation

Charlene BENNETT; Allison WATERS; Julie MORAN; Jeff CONNELL; William HALL; Jaythoon HASSAN

Makale detayları

Predominant Inflammatory and Th1 biased cytokine secretion pre- and post- kidney transplantation

Yazarlar:

Charlene BENNETT | Allison WATERS | Julie MORAN | Jeff CONNELL | William HALL | Jaythoon HASSAN

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

EASTERN JOURNAL OF MEDICINE 2011 Vol.16 Issue 1, pp.22-25

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000773010

Sıklık:

Aylık

eISSN:

1301-0883

pISSN:

1301-0883

Özet:

 


Abstract. A key goal in post-transplant monitoring is the diagnostic detection of harmful processes in the allograft early which can be easily and non-invasively assessed. Cytokines are crucial mediators involved in immune responses leading to rejection. It is known that episodes of viral infections and acute rejection can cause an increase in pro-inflammatory cytokines in transplant recipients. This study is significant since detailed analysis of cytokines was performed in kidney transplant patients pre- and post-transplant to assess the impact of graft implantation. Twenty patients with mean age of 35 years and comprising 8 females who underwent renal transplantation were included in the study.  The mean follow-up time for the study cohort was 5 months. Using a multiplex microassay, twelve cytokines [IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, granulocyte–macrophage colony-stimulating factor, IFN-g and tumour necrosis factor] were measured simultaneously before and after transplant. A strong pro-inflammatory response was seen as the levels of circulating IL-1b (p<0.02) and IL-6 (p<0.01) increased post-transplant.  A Th1 bias was due to increased IFNg (p<0.05) and absent IL-4 and IL-10 post-transplant. Levels of IL-1a, IL-2, IL-7, IL-12, GM-CSF and TNFa remained low and unchanged whilst IL-8 levels was reduced (p<0.02). These findings show a strong pro-inflammatory response with a Th1 cytokine bias and this immunological outcome places the patient at risk of graft rejection. We suggest that diagnostic parameters such as cytokines can be used to monitor allografts non-invasively and may have the potential to guide clinical decisions regarding immunosuppressive therapy which could improve outcomes post-transplantation.

 

Key words:   Kidney transplant, allograft rejection, cytokines, Th1 bias

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Makale detayları

Predominant Inflammatory and Th1 biased cytokine secretion pre- and post- kidney transplantation

Yazarlar: Charlene BENNETT | Allison WATERS | Julie MORAN | Jeff CONNELL | William HALL | Jaythoon HASSAN

Yayıncı: Van Yuzuncu Yıl University

Dergi: EASTERN JOURNAL OF MEDICINE 2011 Vol.16 Issue 1, pp.22-25

Belge Tipi: Makale

Yayın Tarihi: 1/2013

Belge Dili: İngilizce

Document ID (Org No.): 2000773010

Sıklık: Aylık

eISSN: 1301-0883

pISSN: 1301-0883

Sayı

Yazarlar:

Charlene BENNETT | Allison WATERS | Julie MORAN | Jeff CONNELL | William HALL | Jaythoon HASSAN

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

EASTERN JOURNAL OF MEDICINE 2011 Vol.16 Issue 1, pp.22-25

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000773010

Sıklık:

Aylık

eISSN:

1301-0883

pISSN:

1301-0883