Optimization of Synthesis Conditions of Chloramphenicol AptamerFunctionalized DNA Hydrogels

Gülnur Camızcı Aran; Ceren Bayraç

Makale detayları

Optimization of Synthesis Conditions of Chloramphenicol AptamerFunctionalized DNA Hydrogels

Yazarlar:

Gülnur Camızcı Aran | Ceren Bayraç

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Industrial Biotechnology

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.145-157

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284630

pISSN:

2757-5195

Anahtar kelimeler:

Özet:

 Aptamer-functionalized DNA hydrogels are used in many fields due to their high specificity, stability and flexibility. In this study, the synthesis of chloramphenicol-specific aptamer sequence functionalized DNA hydrogel was performed and certain parameters for hydrogel stability were optimized. For the synthesis, the 5 ends of the acrydite modified chloramphenicol-specific aptamer and the partially conjugated DNA strand were attached to the polymer structure as a side branch and held together by hybridization of the complementary regions, hydrogel synthesis was carried out. The optimized parameters were the concentrations of acrydite-modified DNA sequences, the percentage of acrylamide, the molar ratios of linear polymer solutions containing chloramphenicol aptamer and DNA strand 1. In addition, the effects of reaction temperature and the length of the complementary region on gel stability were evaluated. As a result, aptamer-functionalized DNA hydrogel synthesis was completed at 25C with aptamer and DNA strand solutions mixed in 1:1 molar ratio using 40 acrylamide stock solution from 60 linear polyacrylamideDNA conjugation for DNA hydrogel stability. In addition, it was concluded that the length of the conjugate region between the aptamer sequence and the DNA strand increases stability.

 Aptamer-functionalized DNA hydrogels are used in many fields due to their high specificity, stability and flexibility. In this study, the synthesis of chloramphenicol-specific aptamer sequence functionalized DNA hydrogel was performed and certain parameters for hydrogel stability were optimized. For the synthesis, the 5 ends of the acrydite modified chloramphenicol-specific aptamer and the partially conjugated DNA strand were attached to the polymer structure as a side branch and held together by hybridization of the complementary regions, hydrogel synthesis was carried out. The optimized parameters were the concentrations of acrydite-modified DNA sequences, the percentage of acrylamide, the molar ratios of linear polymer solutions containing chloramphenicol aptamer and DNA strand 1. In addition, the effects of reaction temperature and the length of the complementary region on gel stability were evaluated. As a result, aptamer-functionalized DNA hydrogel synthesis was completed at 25C with aptamer and DNA strand solutions mixed in 1:1 molar ratio using 40 acrylamide stock solution from 60 linear polyacrylamideDNA conjugation for DNA hydrogel stability. In addition, it was concluded that the length of the conjugate region between the aptamer sequence and the DNA strand increases stability.

 Aptamer-functionalized DNA hydrogels are used in many fields due to their high specificity, stability and flexibility. In this study, the synthesis of chloramphenicol-specific aptamer sequence functionalized DNA hydrogel was performed and certain parameters for hydrogel stability were optimized. For the synthesis, the 5 ends of the acrydite modified chloramphenicol-specific aptamer and the partially conjugated DNA strand were attached to the polymer structure as a side branch and held together by hybridization of the complementary regions, hydrogel synthesis was carried out. The optimized parameters were the concentrations of acrydite-modified DNA sequences, the percentage of acrylamide, the molar ratios of linear polymer solutions containing chloramphenicol aptamer and DNA strand 1. In addition, the effects of reaction temperature and the length of the complementary region on gel stability were evaluated. As a result, aptamer-functionalized DNA hydrogel synthesis was completed at 25C with aptamer and DNA strand solutions mixed in 1:1 molar ratio using 40 acrylamide stock solution from 60 linear polyacrylamideDNA conjugation for DNA hydrogel stability. In addition, it was concluded that the length of the conjugate region between the aptamer sequence and the DNA strand increases stability.

 Aptamer fonksiyonlandırılmış DNA hidrojelleri yüksek özgünlük, stabilite ve esneklik gibi özellikleri nedeniyle birçok alanda kullanılmaktadır. Bu çalışma kapsamında kloramfenikole özgü aptamer dizisi fonksiyonlandırılmış DNA hidrojeli sentezi gerçekleştirilmiş ve hidrojel stabilitesi için önemli parametreler optimize edilmiştir. Sentez için 5 uçları akridit modifiyeli kloramfenikole özgü aptamer ile ona kısmen eşlenik DNA ipliği polimer yapıya yan dal olarak katılmış ve eşlenik bölgelerin hibridizasyonu ile bir arada tutularak hidrojel sentezi gerçekleştirilmiştir. Optimize edilmiş parametreler akridit modifiyeli DNA dizilerinin konsantrasyonları, akrilamid yüzdesi, kloramfenikol aptameri ve DNA iplik 1 içeren lineer polimer çözeltilerin molar oranlarıdır. Ayrıca, reaksiyon sıcaklığı ve eşlenik bölgenin uzunluğunun jel stabilitesine etkisi değerlendirilmiştir. Sonuç olarak, DNA hidrojel stabilitesi için 60lık lineer poliakrilamid-DNA konjugasyonundan, 40 akrilamid stok çözeltisi kullanılarak 1:1 molar oranda karıştırılan aptamer ve DNA iplik çözeltileri ile 25Cde aptamer fonksiyonlandırılmış DNA hidrojeli sentezi tamamlanmıştır. Bunlara ek olarak, aptamer dizisi ile DNA iplik arasındaki eşlenik bölgenin uzunluğunun stabiliteyi artırdığı sonucuna varılmıştır.

Makale detayları

Optimization of Synthesis Conditions of Chloramphenicol AptamerFunctionalized DNA Hydrogels

Yazarlar: Gülnur Camızcı Aran | Ceren Bayraç

Yayıncı: Çanakkale Onsekiz Mart University

Ana Başlıklar: Industrial Biotechnology

Dergi: Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.145-157

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001284630

pISSN: 2757-5195

Aptamer

DNA hydrogel

chloramphenicol

Aptamer

DNA hidrojel

kloramfenikol

Sayı

Yazarlar:

Gülnur Camızcı Aran | Ceren Bayraç

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Industrial Biotechnology

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.9 Issue 1, pp.145-157

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284630

pISSN:

2757-5195