Investigation of Neuroprotective Activity of Morus Nigra in Mouse Neuroblastoma Cell Culture Line

Dilek Gülce Tatlı

Makale detayları

Investigation of Neuroprotective Activity of Morus Nigra in Mouse Neuroblastoma Cell Culture Line

Yazarlar:

Dilek Gülce Tatlı

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Sinirbilim (Diğer)

Dergi:

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi Vol.12 Issue 2, pp.283-287

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352244

pISSN:

2147-9607

Anahtar kelimeler:

Özet:

 Abstract Aim; Morus Nigra Moraceae is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations 1, 3, 10, 30, 100, 300 and 1000 μgml. The effects on the cells were evaluated using neurotoxicity screening test NTT, apoptosis detection by annexin V method and cell viabilityproliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µgml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT.

 Abstract Aim; Morus Nigra Moraceae is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations 1, 3, 10, 30, 100, 300 and 1000 μgml. The effects on the cells were evaluated using neurotoxicity screening test NTT, apoptosis detection by annexin V method and cell viabilityproliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µgml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT.

 Abstract Aim; Morus Nigra Moraceae is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations 1, 3, 10, 30, 100, 300 and 1000 μgml. The effects on the cells were evaluated using neurotoxicity screening test NTT, apoptosis detection by annexin V method and cell viabilityproliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µgml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT.

 Morus Nigranın Fare Nöroblastoma Hücre Kültür Dizinindeki Nöroprotektif Etkinliğinin Araştırılması D. Gülce TATLI1, Kâmil VURAL1Yeşim Turhan ÖZCAN2, Ertan DARIVERENLİ1 1 Manisa Celal Bayar Üniversitesi Farmakoloji Türkiye 2 İzmir Şehir Hastanesi Çocuk ve Ergen Psikiyatri Türkiye E-mail: D. Gülce TATLI: dgulceuyarhotmail.com Kâmil VURAL: kfsvuralyahoo.com Yeşim Turhan ÖZCAN: yesimturhanozcangmail.com Ertan Darıverenli: ertan6876gmail.com Sorumlu Yazar  Corresponding Author: D. Gülce TATLI Gönderim Tarihi  Received: Kabul Tarihi  Accepted: DOI: 10.34087cbusbed. Öz Giriş ve Amaç: Morus Nigra Moraceae, insanlar tarafından sıkça tüketilen bir meyvedir. Aynı zamanda geleneksel tıpta çeşitli hastalıkların tedavisinde antialerjik, antienflamatuvar, antibakteriyel ve antikanser özellikleri olduğu için kullanıla gelmektedir. Morus Nigra ektresinin hücre canlılığı üzerine etkileri olduğu bazı kaynaklarda ise bu etkisinin olmadığı belirtilmiştir. Bu nedenle Morus Nigra ektresinin Nb2a hücre hattında nörotoksisite, apoptoz ve hücre canlılığı üzerindeki etkilerini incelemek amacıyla yapılmıştır. Gereç ve Yöntemler: Morus Nigranın metanol ekstratları, farklı konsantrasyonlarda 1, 3, 10, 30, 100, 300 ve 1000 μgml Nb2a hücre hattına uygulanmıştır. Nörotoksisite tarama testi NTT, annexin V yöntemiyle apoptoz tespiti ve hücre canlılıkproliferasyon testleri kullanılarak hücre üzerindeki etkiler değerlendirilmiştir. Bulgular: Morus Nigranın metanol ekstratlarının hücre canlılığı ve apoptozis üzerinde belirgin bir etki göstermediğini, ancak nörit uzaması üzerine 100 ve 300 µgml konsantrasyonlarda ılımlı nöroprotektif etki gösterdiği ortaya koymuştur. Sonuç: Bu bulgular, Morus Nigranın antikanser özellikleri olduğu düşünülmemekle birlikte içerdiği fenolik bileşiklerin antioksidan etkileri nedeniyle nöronal sistemdeki aksonları koruyabileceği gösterilmiştir. Anahtar kelimeler: Morus Nigra, Hücre kültürü, Nb2a, apopitozis, NTT Abstract Aim; Morus Nigra Moraceae is a fruit frequently consumed by humans. It is also used in traditional medicine to treat various diseases because it has antiallergic, anti-inflammatory, antibacterial and anticancer properties. It has been stated that Morus Nigra extract has effects on cell viability and some sources do not have this effect. Therefore, this study was carried out to investigate the effects of Morus Nigra extract on neurotoxicity, apoptosis and cell viability in Nb2a cell line Method: Methanol extracts of Morus Nigra were applied to Nb2a cell line at different concentrations 1, 3, 10, 30, 100, 300 and 1000 μgml. The effects on the cells were evaluated using neurotoxicity screening test NTT, apoptosis detection by annexin V method and cell viabilityproliferation tests. Results: It was found that methanol extracts of Morus Nigra had no significant effect on cell viability and apoptosis, but showed moderate neuroprotective effect on neurite elongation at concentrations of 100 and 300 µgml. Conclusion: These findings indicate that although Morus Nigra is not thought to have anticancer properties, it has been shown that the phenolic compounds it contains may protect axons in the neuronal system due to their antioxidant effects. Keywords: Morus Nigra, Cell culture, Nb2a, apoptosis, NTT.

Makale detayları

Investigation of Neuroprotective Activity of Morus Nigra in Mouse Neuroblastoma Cell Culture Line

Yazarlar:

Dilek Gülce Tatlı

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Sinirbilim (Diğer)

Dergi:

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi Vol.12 Issue 2, pp.283-287

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352244

pISSN:

2147-9607

Morus Nigra

Cell culture

Nb2a

Apoptosis

NTTMorus Nigra

Hücre kültürü

Nb2a

apopitozis

NTT

Sayı

Makale detayları

Yazarlar: Dilek Gülce Tatlı

Yayıncı: Manisa Celal Bayar University

Ana Başlıklar: Sinirbilim (Diğer)

Dergi: Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi Vol.12 Issue 2, pp.283-287

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001352244

pISSN: 2147-9607

Sayı

Yazarlar:

Dilek Gülce Tatlı

Yayıncı:

Manisa Celal Bayar University

Ana Başlıklar:

Sinirbilim (Diğer)

Dergi:

Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi Vol.12 Issue 2, pp.283-287

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001352244

pISSN:

2147-9607