Comparative study of mesenchymal stem cells from rat bone marrow and adipose tissue

Qing HE; Zhaoyang YE; Yan ZHOU; Wen-song TAN

Makale detayları

Comparative study of mesenchymal stem cells from rat bone marrow and adipose tissue

Yazarlar:

Qing HE | Zhaoyang YE | Yan ZHOU | Wen-song TAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.477-489

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811161

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 


 
                         Several therapeutic products based on mesenchymal stem cells (MSCs) have been translated into clinical applications.
MSCs should undergo in vitro culture before a sufficient quantity can be achieved. Hence, both expansion kinetics and the biological
characteristics of derived cells from primary culture are pertinent to their applications. In the present study, MSCs were isolated from
rat bone marrow and adipose tissue (designated as bMSCs and aMSCs, respectively) and cells were comparatively analyzed regarding
cell morphology, proliferation, colony formation, differentiation potential, and immunophenotype following the long-term subculture.
No apparent differences could be noticed concerning the morphology between bMSCs and aMSCs. The long-term subculture made
both types of cells smaller, weakened their colony-forming ability, and stimulated the proliferation rate. However, bMSCs demonstrated
better proliferation and colony-forming ability than aMSCs. No significant difference was observed about the expression of some
immunophenotypes (i.e. CD29+/CD90+/CD34-/CD45-) regardless of cell types or population doublings. Notably, bMSCs, but not
aMSCs, maintained the differentiation potential well after the long-term subculture. The present study demonstrates that MSCs derived
from different tissues can be well expanded for the long term, although cells display gradually declined self-renewal and differentiation
potentials to different extents depending on the tissue origins.
                    

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Makale detayları

Comparative study of mesenchymal stem cells from rat bone marrow and adipose tissue

Yazarlar: Qing HE | Zhaoyang YE | Yan ZHOU | Wen-song TAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.477-489

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811161

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Qing HE | Zhaoyang YE | Yan ZHOU | Wen-song TAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.477-489

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811161

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152