Gut Bacterial Microbiota of The Honeybee, Apis mellifera (Hymenoptera: Apidae) with a New Host Record

Mustafa Rustemoglu

Makale detayları

Gut Bacterial Microbiota of The Honeybee, Apis mellifera (Hymenoptera: Apidae) with a New Host Record

Yazarlar:

Mustafa Rustemoglu

Yayıncı:

Igdir University

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.592-599

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284088

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Gut microbial communities of the honeybee, Apis mellifera L. have vital functions in food digestion, providing essential nutrients, improving immune system against pathogens, and detoxifying harmful molecules. The objective of this study was to characterize the core gut bacterial community of newly emerged A. mellifera queens and workers from southeastern Turkey. A total of newly emerged 15 queens and 30 workers were collected from the same apiary. The alimentary tract of collected samples was dissected under sterile conditions. Culture based isolation was performed from the digestive tract of the collected samples to obtain a pure microbe culture. The chemical, morphological and molecular description of each bacteria were accurately done via employment of pure culture. Five bacterial isolates were successfully isolated from the A. mellifera alimentary tract and purified. Molecular and biochemical identification showed that the isolates were Bacillus pumilus, Bacillus subtilis, Bacillus cereus Firmicutes: Bacillaceae MZ540443, MZ540444, MZ540445, Staphylococcus haemolyticus Firmicutes: Staphylococcaceae MZ540447, and Paenibacillus glucanolyticus Firmicutes: Paenibacillaceae MZ540446. This study is the first to report the presence of P. glucanolyticus in gut bacterial community of A. mellifera.

 Gut microbial communities of the honeybee, Apis mellifera L. have vital functions in food digestion, providing essential nutrients, improving immune system against pathogens, and detoxifying harmful molecules. The objective of this study was to characterize the core gut bacterial community of newly emerged A. mellifera queens and workers from southeastern Turkey. A total of newly emerged 15 queens and 30 workers were collected from the same apiary. The alimentary tract of collected samples was dissected under sterile conditions. Culture based isolation was performed from the digestive tract of the collected samples to obtain a pure microbe culture. The chemical, morphological and molecular description of each bacteria were accurately done via employment of pure culture. Five bacterial isolates were successfully isolated from the A. mellifera alimentary tract and purified. Molecular and biochemical identification showed that the isolates were Bacillus pumilus, Bacillus subtilis, Bacillus cereus Firmicutes: Bacillaceae MZ540443, MZ540444, MZ540445, Staphylococcus haemolyticus Firmicutes: Staphylococcaceae MZ540447, and Paenibacillus glucanolyticus Firmicutes: Paenibacillaceae MZ540446. This study is the first to report the presence of P. glucanolyticus in gut bacterial community of A. mellifera.

 Gut microbial communities of the honeybee, Apis mellifera L. have vital functions in food digestion, providing essential nutrients, improving immune system against pathogens, and detoxifying harmful molecules. The objective of this study was to characterize the core gut bacterial community of newly emerged A. mellifera queens and workers from southeastern Turkey. A total of newly emerged 15 queens and 30 workers were collected from the same apiary. The alimentary tract of collected samples was dissected under sterile conditions. Culture based isolation was performed from the digestive tract of the collected samples to obtain a pure microbe culture. The chemical, morphological and molecular description of each bacteria were accurately done via employment of pure culture. Five bacterial isolates were successfully isolated from the A. mellifera alimentary tract and purified. Molecular and biochemical identification showed that the isolates were Bacillus pumilus, Bacillus subtilis, Bacillus cereus Firmicutes: Bacillaceae MZ540443, MZ540444, MZ540445, Staphylococcus haemolyticus Firmicutes: Staphylococcaceae MZ540447, and Paenibacillus glucanolyticus Firmicutes: Paenibacillaceae MZ540446. This study is the first to report the presence of P. glucanolyticus in gut bacterial community of A. mellifera.

 Gut microbial communities of the honeybee, Apis mellifera L. have vital functions in food digestion, providing essential nutrients, improving immune system against pathogens, and detoxifying harmful molecules. The objective of this study was to characterize the core gut bacterial community of newly emerged A. mellifera queens and workers from southeastern Turkey. A total of newly emerged 15 queens and 30 workers were collected from the same apiary. The alimentary tract of collected samples was dissected under sterile conditions. Culture based isolation was performed from the digestive tract of the collected samples to obtain a pure microbe culture. The chemical, morphological and molecular description of each bacteria were accurately done via employment of pure culture. Five bacterial isolates were successfully isolated from the A. mellifera alimentary tract and purified. Molecular and biochemical identification showed that the isolates were Bacillus pumilus, Bacillus subtilis, Bacillus cereus Firmicutes: Bacillaceae MZ540443, MZ540444, MZ540445, Staphylococcus haemolyticus Firmicutes: Staphylococcaceae MZ540447, and Paenibacillus glucanolyticus Firmicutes: Paenibacillaceae MZ540446. This study is the first to report the presence of P. glucanolyticus in gut bacterial community of A. mellifera.

Makale detayları

Gut Bacterial Microbiota of The Honeybee, Apis mellifera (Hymenoptera: Apidae) with a New Host Record

Yazarlar:

Mustafa Rustemoglu

Yayıncı:

Igdir University

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.592-599

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284088

pISSN:

2536-4618

microbiota

Southeast Turkey

Paenibacillus glucanolyticus

new host

honeybee

microbiota

Southeast Turkey

Paenibacillus glucanolyticus

new host

honeybee

Sayı

Makale detayları

Yazarlar: Mustafa Rustemoglu

Yayıncı: Igdir University

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.592-599

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001284088

pISSN: 2536-4618

Sayı

Yazarlar:

Mustafa Rustemoglu

Yayıncı:

Igdir University

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 1, pp.592-599

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284088

pISSN:

2536-4618