Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Mohammad Sadat Hosseini GROUH; Naeimeh SOUSARAEI; Mohammad AKBARI; Vahid RAHIMI; Hamidreza BAYAT

Makale detayları

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Yazarlar:

Mohammad Sadat Hosseini GROUH | Naeimeh SOUSARAEI | Mohammad AKBARI | Vahid RAHIMI | Hamidreza BAYAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2015 Vol.39 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000807050

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. Anthers collected from the pollen donor were irradiated by X-ray with doses of 0, 100, 200, 300, and 400 Gy in 2012. A second round of experiments was conducted in 2013 with 300 and 400 Gy. The ovaries from each pollinated flower were collected and cultured and the produced plantlets were then acclimatized. Chromosome counting and flow cytometry were used to determine the ploidy levels. The viability of irradiated pollens was also evaluated. Pollination with irradiated pollen at doses ranging from 100 to 400 Gy can significantly reduce fruit set, particularly at higher doses (300 and 400 Gy). The embryo production was reduced in I. pseudacorus as doses increased; the highest doses led to reduction in the number of regenerants with the formation of just 6 and 4 embryos at 300 and 400 Gy, respectively. Haploid plantlets were produced when the pollen grains were exposed to 300 and 400 Gy of X-ray. Irradiation at 100 and 200 Gy was found to be insufficient to cause pollen sterility. The best dose for haploid production in I. pseudacorus is hereby found to be 300 or 400 Gy of X-ray.

 

This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. Anthers collected from the pollen donor were irradiated by X-ray with doses of 0, 100, 200, 300, and 400 Gy in 2012. A second round of experiments was conducted in 2013 with 300 and 400 Gy. The ovaries from each pollinated flower were collected and cultured and the produced plantlets were then acclimatized. Chromosome counting and flow cytometry were used to determine the ploidy levels. The viability of irradiated pollens was also evaluated. Pollination with irradiated pollen at doses ranging from 100 to 400 Gy can significantly reduce fruit set, particularly at higher doses (300 and 400 Gy). The embryo production was reduced in I. pseudacorus as doses increased; the highest doses led to reduction in the number of regenerants with the formation of just 6 and 4 embryos at 300 and 400 Gy, respectively. Haploid plantlets were produced when the pollen grains were exposed to 300 and 400 Gy of X-ray. Irradiation at 100 and 200 Gy was found to be insufficient to cause pollen sterility. The best dose for haploid production in I. pseudacorus is hereby found to be 300 or 400 Gy of X-ray.

Makale detayları

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Yazarlar:

Mohammad Sadat Hosseini GROUH | Naeimeh SOUSARAEI | Mohammad AKBARI | Vahid RAHIMI | Hamidreza BAYAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2015 Vol.39 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000807050

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Line

flow cytometry

chromosome counting

X-ray irradiation

embryo rescue

Sayı

Makale detayları

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Yazarlar: Mohammad Sadat Hosseini GROUH | Naeimeh SOUSARAEI | Mohammad AKBARI | Vahid RAHIMI | Hamidreza BAYAT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2015 Vol.39 Issue 4

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000807050

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Line

flow cytometry

chromosome counting

X-ray irradiation

embryo rescue

Sayı

Yazarlar:

Mohammad Sadat Hosseini GROUH | Naeimeh SOUSARAEI | Mohammad AKBARI | Vahid RAHIMI | Hamidreza BAYAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2015 Vol.39 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000807050

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X