Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Ahmet ONAY

Makale detayları

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Yazarlar:

Ahmet ONAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000809778

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog  media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic mass-es  were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with 0.5-4 mg/l benzylaminop-urine.  After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somat-ic  embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured  somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets.  Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N 6 -benzyladenine); EMS -embryogenic mass; IAA  - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - a- naphthalene acetic acid; PGR - plant growth regulator; SE(s) -somatic  embryo (s);.

 

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog  media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic mass-es  were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with 0.5-4 mg/l benzylaminop-urine.  After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somat-ic  embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured  somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets.  Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N 6 -benzyladenine); EMS -embryogenic mass; IAA  - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - a- naphthalene acetic acid; PGR - plant growth regulator; SE(s) -somatic  embryo (s);.

Makale detayları

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Yazarlar:

Ahmet ONAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000809778

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Turk. J. Agric. For.

24

(2000)

465-474. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.24

iss.4.

Sayı

Makale detayları

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Yazarlar: Ahmet ONAY

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000809778

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Turk. J. Agric. For.

24

(2000)

465-474. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.24

iss.4.

Sayı

Yazarlar:

Ahmet ONAY

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000809778

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X