An ultrasensitive electrochemical immunosensor platform based on   disposable ITO electrode modified by 3-CPTMS for early detection of   parathyroid hormone

İnci ULUDAĞ; Mustafa Kemal SEZGİNTÜRK

Makale detayları

An ultrasensitive electrochemical immunosensor platform based on disposable ITO electrode modified by 3-CPTMS for early detection of parathyroid hormone

Yazarlar:

İnci ULUDAĞ | Mustafa Kemal SEZGİNTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1697-1710

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725553

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Parathyroid hormone (PTH) is a polypeptide containing 84   amino acids secreted by cells of the parathyroid glands. Imbalances of   parathyroid levels cause medical problems such as osteoporosis, mental   disorders, pancreatitis, kidney stones, cancer, and other symptoms. In this   study, we aimed to design an ultrasensitive electrochemical immunosensor for   PTH detection. Indium tin oxide (ITO) was used as an electrode for   electrochemical impedance spectroscopy (EIS) measurements. ITO sheets were   modified by using 3-cyanopropyltrimethoxysilane (3-CPTMS) self-assembled   monolayers (SAMs) for immobilizing the anti-PTH antibody via covalent   interactions. Cyclic voltammetry (CV) and EIS methods were applied to   characterize immobilization steps. Therefore, 1% was selected for an   optimal concentration of silane, 10 ng/mL was selected as an optimal   concentration of anti-PTH, and 30 and 45 min were selected as optimal   incubation times for anti-PTH and PTH, respectively. PTH antigen was   determined in the concentration range from 0.05 fg/mL to 150 fg/mL. To   detect the analytical characterization of the 3-CPTMS modified immunosensor,   linear range, repeatability, reproducibility, Kramers-Kronig transform, and   regeneration studies were performed. Also, the shelf life of the developed   biosensor was investigated. Finally, real human serum samples were analyzed   with the PTH immunosensor. The results showed that the designed biosensor   system has high potential for early detection for medical treatments.

Makale detayları

An ultrasensitive electrochemical immunosensor platform based on disposable ITO electrode modified by 3-CPTMS for early detection of parathyroid hormone

Yazarlar: İnci ULUDAĞ | Mustafa Kemal SEZGİNTÜRK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1697-1710

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000725553

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

İnci ULUDAĞ | Mustafa Kemal SEZGİNTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1697-1710

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725553

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527