Baicalein Has Protective Effect in H2O2-induced L929 Cell Damage

Pınar Bayram

Makale detayları

Baicalein Has Protective Effect in H2O2-induced L929 Cell Damage

Yazarlar:

Pınar Bayram

Yayıncı:

Ankara University

Ana Başlıklar:

Histology and Embryology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.11-16

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349822

pISSN:

0365-8104

Anahtar kelimeler:

Özet:

 Objectives: In this study, it was aimed to investigate the possible protective effects of baicalein in H2O2-induced L929 fibroblast cell model by determining the parameters of oxidative stress SOD and inflammation process TNF-alpha, TGF-beta, IL-10. Materials and Methods: Baicalein was applied at different doses 200, 100 and 80, μM to L929 fibroblast cells. After 23 hours of incubation, 1 mM H2O2 was added and cell viability was test by CVDK8. Then, fluorescent diacetate FDA-propidium iodide PI staining was performed to determine the ratio of live and dead cells. The levels of TNF-alpha, IL10, TGF-beta and the activity of SOD were determined by ELISA. Results: After 1 h incubation with 1 mM H2O2, the viability decreased by approximately 77. It was observed that cell viability increased the most in the B100 group, withwithout H2O2. The cell viability increased after 24 h of baicalein and H2O2 co-incubation. The 100 μM baicalein application reduced the damage of H2O2 up to 63. When the levels of TNF-alpha were examined, a significant increase was observed in the H2O2-damage group compared to control group. Additionally, the levels of IL-10 and TGF-beta increased in H2O2-damage group compared to control group; however, the levels of those cytokines were decreased in baicalein application groups. Conclusion: Pretreatment with baicalein especially 200 μM and 100 μM results in significant findings including the suppression of inflammatory cytokines, TNF-alpha, TGF-beta, IL-10 and increase of SOD parameter, SOD, in L929 cells. Taking everything into account, baicalein which is a natural product may be an alternative therapeutic option for prevention of cellular SOD and inflammatory processes. To better understand the potential beneficial effects of baicalein, molecular mechanisms and cellular targets should be investigated.

 Objectives: In this study, it was aimed to investigate the possible protective effects of baicalein in H2O2-induced L929 fibroblast cell model by determining the parameters of oxidative stress SOD and inflammation process TNF-alpha, TGF-beta, IL-10. Materials and Methods: Baicalein was applied at different doses 200, 100 and 80, μM to L929 fibroblast cells. After 23 hours of incubation, 1 mM H2O2 was added and cell viability was test by CVDK8. Then, fluorescent diacetate FDA-propidium iodide PI staining was performed to determine the ratio of live and dead cells. The levels of TNF-alpha, IL10, TGF-beta and the activity of SOD were determined by ELISA. Results: After 1 h incubation with 1 mM H2O2, the viability decreased by approximately 77. It was observed that cell viability increased the most in the B100 group, withwithout H2O2. The cell viability increased after 24 h of baicalein and H2O2 co-incubation. The 100 μM baicalein application reduced the damage of H2O2 up to 63. When the levels of TNF-alpha were examined, a significant increase was observed in the H2O2-damage group compared to control group. Additionally, the levels of IL-10 and TGF-beta increased in H2O2-damage group compared to control group; however, the levels of those cytokines were decreased in baicalein application groups. Conclusion: Pretreatment with baicalein especially 200 μM and 100 μM results in significant findings including the suppression of inflammatory cytokines, TNF-alpha, TGF-beta, IL-10 and increase of SOD parameter, SOD, in L929 cells. Taking everything into account, baicalein which is a natural product may be an alternative therapeutic option for prevention of cellular SOD and inflammatory processes. To better understand the potential beneficial effects of baicalein, molecular mechanisms and cellular targets should be investigated.

 Objectives: In this study, it was aimed to investigate the possible protective effects of baicalein in H2O2-induced L929 fibroblast cell model by determining the parameters of oxidative stress SOD and inflammation process TNF-alpha, TGF-beta, IL-10. Materials and Methods: Baicalein was applied at different doses 200, 100 and 80, μM to L929 fibroblast cells. After 23 hours of incubation, 1 mM H2O2 was added and cell viability was test by CVDK8. Then, fluorescent diacetate FDA-propidium iodide PI staining was performed to determine the ratio of live and dead cells. The levels of TNF-alpha, IL10, TGF-beta and the activity of SOD were determined by ELISA. Results: After 1 h incubation with 1 mM H2O2, the viability decreased by approximately 77. It was observed that cell viability increased the most in the B100 group, withwithout H2O2. The cell viability increased after 24 h of baicalein and H2O2 co-incubation. The 100 μM baicalein application reduced the damage of H2O2 up to 63. When the levels of TNF-alpha were examined, a significant increase was observed in the H2O2-damage group compared to control group. Additionally, the levels of IL-10 and TGF-beta increased in H2O2-damage group compared to control group; however, the levels of those cytokines were decreased in baicalein application groups. Conclusion: Pretreatment with baicalein especially 200 μM and 100 μM results in significant findings including the suppression of inflammatory cytokines, TNF-alpha, TGF-beta, IL-10 and increase of SOD parameter, SOD, in L929 cells. Taking everything into account, baicalein which is a natural product may be an alternative therapeutic option for prevention of cellular SOD and inflammatory processes. To better understand the potential beneficial effects of baicalein, molecular mechanisms and cellular targets should be investigated.

 Amaç: Mevcut çalışmada, Baicaleinin H2O2 ile indüklenmiş L929 fibroblast hücre hasarı modelinde oksidatif stres SOD ve enflamasyon süreci TNFalfa, TGF-beta, IL-10 parametreleri üzerine olan koruyucu etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: L929 fibroblast hücrelerine farklı dozlarda 200, 100 ve 80 μM Baicalein uygulandı. Yirmi üç saatlik inkübasyonun ardından 1 mM H2O2 eklendi ve hücre canlılığı CVDK8 ile test edildi. Daha sonra canlı ve ölü hücrelerin oranını belirlemek için floresan diasetat FDA-propidyum iyodür PI boyaması yapıldı. TNF-alfa, IL10, TGF-beta seviyeleri ve SOD aktivitesi ELISA ile belirlendi. Bulgular: 1 mM H2O2 ile 1 saatlik inkübasyondan sonra hücre canlılığı yaklaşık 77 oranında azaldı. Hasarsız ya da H2O2 hasarlı gruplarda, hücre canlılığında artışın en fazla B100 grubunda olduğu görüldü. Baicalein ve H2O2nin birlikte inkübasyonundan 24 saat sonra hücre canlılığının arttığı tespit edildi. 100 μM Baicalein uygulaması, H2O2nin hücreleri öldürme oranını 63e kadar azalttı. TNF-alfa düzeyleri incelendiğinde H2O2-hasarlı grupta kontrol grubuna göre anlamlı artış gözlendi. Ek olarak, IL-10 ve TGF-beta seviyeleri H2O2-hasarlı grupta kontrol grubuna göre arttı; ancak bu sitokinlerin seviyeleri Baicalein uygulama gruplarında azaldı. Sonuç: Baicalein özellikle 200 μM ve 100μM uygulaması sonucunda L929 hücrelerinde enflamatuvar sitokinler olan TNF-alfa, TGF-beta, IL- 10un baskılanması ve SOD parametresi olan SODnin artması gibi önemli bulgular elde edilmiştir. Tüm veriler ışığında, doğal bir etken madde olan baicalein, hücresel SOD ve enflamatuvar süreçlerin önlenmesi için alternatif bir tedavi seçeneği potansiyeline sahiptir. Baicaleinin potansiyel yararlı etkilerini daha iyi anlamak için moleküler mekanizmalar ve hücresel hedeflerin araştırılmasına yönelik yeni çalışmalara ihtiyaç vardır.

Makale detayları

Baicalein Has Protective Effect in H2O2-induced L929 Cell Damage

Yazarlar:

Pınar Bayram

Yayıncı:

Ankara University

Ana Başlıklar:

Histology and Embryology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.11-16

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349822

pISSN:

0365-8104

Baicalein

Hydrogen Peroxide

Inflammation

Oxidative Stress

L929Baicalein

Hidrojen Peroksit

Enflamasyon

Oksidatif Stres

L929

Sayı

Makale detayları

Yazarlar: Pınar Bayram

Yayıncı: Ankara University

Ana Başlıklar: Histology and Embryology

Dergi: Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.11-16

Belge Tipi: Makale

Yayın Tarihi: 5/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001349822

pISSN: 0365-8104

Sayı

Yazarlar:

Pınar Bayram

Yayıncı:

Ankara University

Ana Başlıklar:

Histology and Embryology

Dergi:

Ankara Üniversitesi Tıp Fakültesi Mecmuası Vol.76 Issue 1, pp.11-16

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001349822

pISSN:

0365-8104