Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Sedat ÇETİN; Veysel YÜKSEK; Semiha DEDE; Ayşe USTA; Mehmet TAŞPINAR

Makale detayları

Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Yazarlar:

Sedat ÇETİN | Veysel YÜKSEK | Semiha DEDE | Ayşe USTA | Mehmet TAŞPINAR

Yayıncı:

Ebubekir CEYLAN

Ana Başlıklar:

Hayvancılık

Dergi:

Turkish Journal of Veterinary Research 2020 Vol.4 Issue 2, pp.45-49

Belge Tipi:

Makale

Yayın Tarihi:

10/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000639565

Sıklık:

İki Yıllık

eISSN:

2602-3695

Özet:

 

Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell.
Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of  NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method.
Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while it increased 7 times in the group treated with  NaF+lycopene. A significant difference was detected between the groups in terms of gene expression pattern. While the Ripk 3 gene increased slightly in the 24th hour applied lycopene group, it was observed that only lycopene applied group increased 8 times and  NaF+lycopene applied group increased in the 9 times 
Conclusion: Based on the results obtained from this study, it was seen that activation of necrotic genes is important in explaining the molecular basis of cell death from NaF, which is applied as fluorine source, in revealing the molecular basis of the necrotic pathway. It was found that the decrease in cell viability due to NaF increased with lycopene, but the use of lycopene with fluorine also increased necrotic gene expression.

Keywords: NaF, in vitro, Lycopene, Necrotic Genes.

Makale detayları

Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Yazarlar: Sedat ÇETİN | Veysel YÜKSEK | Semiha DEDE | Ayşe USTA | Mehmet TAŞPINAR

Yayıncı: Ebubekir CEYLAN

Ana Başlıklar: Hayvancılık

Dergi: Turkish Journal of Veterinary Research 2020 Vol.4 Issue 2, pp.45-49

Belge Tipi: Makale

Yayın Tarihi: 10/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000639565

Sıklık: İki Yıllık

eISSN: 2602-3695

Sayı

Yazarlar:

Sedat ÇETİN | Veysel YÜKSEK | Semiha DEDE | Ayşe USTA | Mehmet TAŞPINAR

Yayıncı:

Ebubekir CEYLAN

Ana Başlıklar:

Hayvancılık

Dergi:

Turkish Journal of Veterinary Research 2020 Vol.4 Issue 2, pp.45-49

Belge Tipi:

Makale

Yayın Tarihi:

10/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000639565

Sıklık:

İki Yıllık

eISSN:

2602-3695