Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding

DUSICA DORIC; VLADISLAV OGNJANOV; GORAN BARAC; MIRJANA LJUBOJEVIC; ANKICA PRANJIC; KRUNOSLAV DUGALIC; SEZAİ ERCİŞLİ

Makale detayları

Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding

Yazarlar:

DUSICA DORIC | VLADISLAV OGNJANOV | GORAN BARAC | MIRJANA LJUBOJEVIC | ANKICA PRANJIC | KRUNOSLAV DUGALIC | SEZAİ ERCİŞLİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.575-581

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811883

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Prunus cerasus L. Obla?∈ska?sourcherry≥rmplasmwasestablished∈vitrodirectlyom∈situplantsond⇔erentexplantcol≤ctiondates,enabl∈gquickclonalμ<iplicationand∫roduction→as√→ckbreed∈gprogram.Rosee∈itiationoffour∈vestigated≥¬ypeswaspossib≤omNovember→Aprilonthemediumconta∈∈gSchenkandHildebrandt(SH)macroe≤ments,Murashi≥andSk∞g(MS)microe≤ments,andvitamins⊃p≤mentedwith(∈mgL?1)6−benzyladen∈e(BA),0.5;∈do≤−3−butyricac,0.01;gierellicac,0.1;citricac10;andL−ascorbicac10.Thelowestpercentofcontaminationwas¬ed∈NovemberandDecember,whendormantbudswereusedasanexplantsource,andthehighestpercenta≥wasfoundwithactivelygrow∈gsh∞ttips.Theelongationphaseofrosees∈itiatedomdormantbudsrema∈edamajorobstac≤.Survivalratesofsh∞ttipsobta∈ed∈Aprilwerehighand⊂sequentgrowthmoreprominent.An∈creasing∈dexofμ<iplicationom1.5→1.9was¬edonDriverandKuniyukiwalnutmedium(DKW)with0.8mgL?1BAand0.01mgL?1IBA∈theObla?∈ska?sourcherry≥rmplasmwasestablished∈vitrodirectlyom∈situplantsond⇔erentexplantcol≤ctiondates,enabl∈gquickclonalμ<iplicationand∫roduction→a→sckbreed∈gprogram.Rosee∈itiationoffour∈vestigated≥¬ypeswaspossib≤omNovember→Aprilonthemediumconta∈∈gSchenkandHildebrandt(SH)macroe≤ments,Murashi≥andSk∞g(MS)microe≤ments,andvitamins⊃p≤mentedwith(∈mgL?1)6-benzyladen∈e(BA),0.5;∈do≤-3-butyricac,0.01;gierellicac,0.1;citricac10;andL-ascorbicac10.Thelowestpercentofcontaminationwas¬ed∈NovemberandDecember,whendormantbudswereusedasanexplantsource,andthehighestpercenta≥wasfoundwithactivelygrow∈gsh∞ttips.Theelongationphaseofrosees∈itiatedomdormantbudsrema∈edamajorobstac≤.Survivalratesofsh∞ttipsobta∈ed∈Aprilwerehighand⊂sequentgrowthmoreprominent.An∈creasing∈dexofμ<iplicationom1.5→1.9was¬edonDriverandKuniyukiwalnutmedium(DKW)with0.8mgL?1BAand0.01mgL?1IBA∈theObla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium (DKW) with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in OV17?andOV17?andOV 17? and OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA.

 

Prunus cerasus L. Obla?∈ska?sourcherry≥rmplasmwasestablished∈vitrodirectlyom∈situplantsond⇔erentexplantcol≤ctiondates,enabl∈gquickclonalμ<iplicationand∫roduction→as√→ckbreed∈gprogram.Rosee∈itiationoffour∈vestigated≥¬ypeswaspossib≤omNovember→Aprilonthemediumconta∈∈gSchenkandHildebrandt(SH)macroe≤ments,Murashi≥andSk∞g(MS)microe≤ments,andvitamins⊃p≤mentedwith(∈mgL?1)6−benzyladen∈e(BA),0.5;∈do≤−3−butyricac,0.01;gierellicac,0.1;citricac10;andL−ascorbicac10.Thelowestpercentofcontaminationwas¬ed∈NovemberandDecember,whendormantbudswereusedasanexplantsource,andthehighestpercenta≥wasfoundwithactivelygrow∈gsh∞ttips.Theelongationphaseofrosees∈itiatedomdormantbudsrema∈edamajorobstac≤.Survivalratesofsh∞ttipsobta∈ed∈Aprilwerehighand⊂sequentgrowthmoreprominent.An∈creasing∈dexofμ<iplicationom1.5→1.9was¬edonDriverandKuniyukiwalnutmedium(DKW)with0.8mgL?1BAand0.01mgL?1IBA∈theObla?∈ska?sourcherry≥rmplasmwasestablished∈vitrodirectlyom∈situplantsond⇔erentexplantcol≤ctiondates,enabl∈gquickclonalμ<iplicationand∫roduction→a→sckbreed∈gprogram.Rosee∈itiationoffour∈vestigated≥¬ypeswaspossib≤omNovember→Aprilonthemediumconta∈∈gSchenkandHildebrandt(SH)macroe≤ments,Murashi≥andSk∞g(MS)microe≤ments,andvitamins⊃p≤mentedwith(∈mgL?1)6-benzyladen∈e(BA),0.5;∈do≤-3-butyricac,0.01;gierellicac,0.1;citricac10;andL-ascorbicac10.Thelowestpercentofcontaminationwas¬ed∈NovemberandDecember,whendormantbudswereusedasanexplantsource,andthehighestpercenta≥wasfoundwithactivelygrow∈gsh∞ttips.Theelongationphaseofrosees∈itiatedomdormantbudsrema∈edamajorobstac≤.Survivalratesofsh∞ttipsobta∈ed∈Aprilwerehighand⊂sequentgrowthmoreprominent.An∈creasing∈dexofμ<iplicationom1.5→1.9was¬edonDriverandKuniyukiwalnutmedium(DKW)with0.8mgL?1BAand0.01mgL?1IBA∈theObla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium (DKW) with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in OV17?andOV17?andOV 17? and OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA.

Makale detayları

Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding

Yazarlar:

DUSICA DORIC | VLADISLAV OGNJANOV | GORAN BARAC | MIRJANA LJUBOJEVIC | ANKICA PRANJIC | KRUNOSLAV DUGALIC | SEZAİ ERCİŞLİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.575-581

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811883

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Dormant period

elongation

explant collection date

Prunus cerasus L.

rooting

Sayı

Makale detayları

Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding

Yazarlar: DUSICA DORIC | VLADISLAV OGNJANOV | GORAN BARAC | MIRJANA LJUBOJEVIC | ANKICA PRANJIC | KRUNOSLAV DUGALIC | SEZAİ ERCİŞLİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.575-581

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811883

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Dormant period

elongation

explant collection date

Prunus cerasus L.

rooting

Sayı

Yazarlar:

DUSICA DORIC | VLADISLAV OGNJANOV | GORAN BARAC | MIRJANA LJUBOJEVIC | ANKICA PRANJIC | KRUNOSLAV DUGALIC | SEZAİ ERCİŞLİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.575-581

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811883

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152